Results 31 to 40 of about 43,196 (233)
Characterizing inactive ribosomes in translational profiling [PDF]
Translation, 2016 The broad impact of translational regulation has emerged explosively in the last few years in part due to the technological advance in genome-wide interrogation of gene expression. During mRNA translation, the majority of actively translating ribosomes exist as polysomes in cells with multiple ribosomes loaded on a single transcript.
Botao, Liu, Shu-Bing, Qian
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eLife, 2020 Ribosomal profiling has shed new light on how ribosomes can ignore stop codons in messenger RNA.
Kim M Keeling, David M Bedwell
doaj +1 more source
HRPDviewer: human ribosome profiling data viewer [PDF]
Database, 2018 Translational regulation plays an important role in protein synthesis. Dysregulation of translation causes abnormal cell physiology and leads to diseases such as inflammatory disorders and cancers. An emerging technique, called ribosome profiling (ribo-seq), was developed to capture a snapshot of translation.
Wei-Sheng Wu +8 more
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The awesome power of ribosome profiling [PDF]
RNA, 2015 Ribosome profiling has proved to be a fantastic innovation which has revealed so much about mRNA translation that had previously gone unrecognized. The RNA community owes Jonathan Weissman and Nick Ingolia a deep sense of gratitude for conceiving the approach in the first place, and for their detailed optimization of the methodology. The origins of the
Jackson, Richard, Standart, Nancy
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eLife, 2022 Protein methylation occurs predominantly on lysine and arginine residues, but histidine also serves as a methylation substrate. However, a limited number of enzymes responsible for this modification have been reported.
Eriko Matsuura-Suzuki +12 more
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Multiplexing polysome profiling experiments to study translation in Escherichia coli.
PLoS ONE, 2019 Polysome profiling is a widely used method to monitor the translation status of mRNAs. Although it is theoretically a simple technique, it is labor intensive.
Huong Le Nguyen +4 more
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A Rapid Ribosome Profiling Method Elucidates Chloroplast Ribosome Behavior in Vivo [PDF]
The Plant Cell, 2013 Abstract The profiling of ribosome footprints by deep sequencing has revolutionized the analysis of translation by mapping ribosomes with high resolution on a genome-wide scale. We present a variation on this approach that offers a rapid and cost-effective alternative for the genome-wide profiling of chloroplast ribosomes.
Zoschke, R., Watkins, K., Barkan, A.
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Illuminating Parasite Protein Production by Ribosome Profiling [PDF]
Trends in Parasitology, 2016 While technologies for global enumeration of transcript abundance are well-developed, those that assess protein abundance require tailoring to penetrate to low-abundance proteins. Ribosome profiling circumvents this challenge by measuring global protein production via sequencing small mRNA fragments protected by the assembled ribosome.
Marilyn, Parsons, Peter J, Myler
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FEBS Letters, EarlyView.This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva +5 more
wiley +1 more source
Clarifying the Translational Pausing Landscape in Bacteria by Ribosome Profiling
Cell Reports, 2016 The rate of protein synthesis varies according to the mRNA sequence in ways that affect gene expression. Global analysis of translational pausing is now possible with ribosome profiling.
Fuad Mohammad +3 more
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