Results 1 to 10 of about 28,198 (287)
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
Nature Communications, 2020 RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate ...
Johan O. L. Andreasson +3 more
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Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme
Nature Communications, 2017 Twister-sister is a self-cleaving ribozyme. Here, the authors report the 2.0 Å crystal structure of the four-way junctional twister-sister ribozyme in the pre-catalytic state and discuss mechanistic implications based on their mutagenesis experiments and
Luqian Zheng +7 more
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The Hammerhead Ribozyme: A Long History for a Short RNA
Molecules, 2017 Small nucleolytic ribozymes are a family of naturally occurring RNA motifs that catalyse a self-transesterification reaction in a highly sequence-specific manner.
Marcos de la Peña +2 more
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A recombinase-activated ribozyme to knock down endogenous gene expression in zebrafish. [PDF]
PLoS GeneticsPrecise regulation of gene expression is essential to understand a wide range of biological processes. Control over gene expression can be achieved using site-directed recombinases and endonucleases whose efficiency is variable and dependent on the ...
Thomas Juan +6 more
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A hammerhead ribozyme selects mechanically stable conformations for catalysis against viral RNA [PDF]
Communications BiologyRibozymes, widely found in prokaryotes and eukaryotes, target nucleic acids and can be engineered as biotechnical tools or for gene regulation or immune therapy. Among them, hammerhead is the smallest and best characterized ribozyme.
Man Lu +11 more
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A Faster Triphosphorylation Ribozyme. [PDF]
PLoS ONE, 2015 In support of the RNA world hypothesis, previous studies identified trimetaphosphate (Tmp) as a plausible energy source for RNA world organisms. In one of these studies, catalytic RNAs (ribozymes) that catalyze the triphosphorylation of RNA 5'-hydroxyl ...
Gregory F Dolan +2 more
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Ribozyme-catalysed RNA synthesis using triplet building blocks
eLife, 2018 RNA-catalyzed RNA replication is widely believed to have supported a primordial biology. However, RNA catalysis is dependent upon RNA folding, and this yields structures that can block replication of such RNAs.
James Attwater +4 more
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The structure and catalytic mechanism of a pseudoknot-containing hammerhead ribozyme [PDF]
Nature CommunicationsWe have determined the crystal structure of a pseudoknot (PK)-containing hammerhead ribozyme that closely resembles the pistol ribozyme, with essentially identical secondary structure and connectivity. The activity is more sensitive to deletion of the G8
Xuelin Zhan +6 more
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Engineering of RNase P Ribozymes for Therapy against Human Cytomegalovirus Infection [PDF]
VirusesNucleic acid-based gene interference and editing strategies, such as antisense oligonucleotides, ribozymes, RNA interference (RNAi), and CRISPR/Cas9 coupled with guide RNAs, are exciting research tools and show great promise for clinical applications in ...
Adam Smith +3 more
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Molecules, 2023 The multimolecular assembly of three-dimensionally structured proteins forms their quaternary structures, some of which have high geometric symmetry. The size and complexity of protein quaternary structures often increase in a hierarchical manner, with ...
Mst. Ayesha Siddika +6 more
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