Results 1 to 10 of about 43,119 (293)
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme [PDF]
Nature Communications, 2020 RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate ...
Johan O. L. Andreasson +3 more
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A Faster Triphosphorylation Ribozyme. [PDF]
PLoS ONE, 2015 In support of the RNA world hypothesis, previous studies identified trimetaphosphate (Tmp) as a plausible energy source for RNA world organisms. In one of these studies, catalytic RNAs (ribozymes) that catalyze the triphosphorylation of RNA 5'-hydroxyl ...
Gregory F Dolan +2 more
doaj +13 more sources
RNase P Ribozymes Inhibit the Replication of Human Cytomegalovirus by Targeting Essential Viral Capsid Proteins [PDF]
Viruses, 2015 An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
Zhu Yang +9 more
doaj +5 more sources
A recombinase-activated ribozyme to knock down endogenous gene expression in zebrafish. [PDF]
PLoS GeneticsPrecise regulation of gene expression is essential to understand a wide range of biological processes. Control over gene expression can be achieved using site-directed recombinases and endonucleases whose efficiency is variable and dependent on the ...
Thomas Juan +6 more
doaj +2 more sources
A hammerhead ribozyme selects mechanically stable conformations for catalysis against viral RNA [PDF]
Communications BiologyRibozymes, widely found in prokaryotes and eukaryotes, target nucleic acids and can be engineered as biotechnical tools or for gene regulation or immune therapy. Among them, hammerhead is the smallest and best characterized ribozyme.
Man Lu +11 more
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Computational De Novo Design of Group II Introns Yields Highly Active Ribozymes. [PDF]
ChembiochemGroup II Introns (G2Is) are large self‐splicing ribozymes with promising biotechnological applications. This study utilized RNA inverse folding to design three novel G2Is. The designed intron Arq.I2, an unexpectedly proficient ribozyme, self‐splices not only in vitro, but also in Escherichia coli cells.
Szokoli D +3 more
europepmc +2 more sources
The structure and catalytic mechanism of a pseudoknot-containing hammerhead ribozyme [PDF]
Nature CommunicationsWe have determined the crystal structure of a pseudoknot (PK)-containing hammerhead ribozyme that closely resembles the pistol ribozyme, with essentially identical secondary structure and connectivity. The activity is more sensitive to deletion of the G8
Xuelin Zhan +6 more
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Molecules, 2023 The multimolecular assembly of three-dimensionally structured proteins forms their quaternary structures, some of which have high geometric symmetry. The size and complexity of protein quaternary structures often increase in a hierarchical manner, with ...
Mst. Ayesha Siddika +6 more
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Zoonotic Diseases, 2023 Developing novel antiviral agents and approaches is essential for the treatment against human and zoonotic viruses. We had previously produced RNase P-based ribozyme variants capable of efficiently cleaving mRNA in vitro.
Bin Yan +4 more
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eLife, 2021 Ribozyme switches are a class of RNA-encoded genetic switch that support conditional regulation of gene expression across diverse organisms. An improved elucidation of the relationships between sequence, structure, and activity can improve our capacity ...
Calvin M Schmidt, Christina D Smolke
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