Results 1 to 10 of about 18,585 (206)

RNA-peptide interactions tune the ribozyme activity within coacervate microdroplet dispersions [PDF]

Nature Communications
Membrane-free complex coacervate microdroplets are compelling models for primitive compartmentalisation with the ability to form from biological molecules.
Basusree Ghosh, Patrick M. McCall, Kristian Kyle Le Vay, Archishman Ghosh, Lars Hubatsch, David T. Gonzales, Jan Brugués, Hannes Mutschler, T-Y. Dora Tang   +8 more
doaj   +2 more sources

Ribozyme-Mediated Knockdown of lncRNA Gene Expression in Drosophila [PDF]

Bio-Protocol
Long noncoding RNAs (lncRNAs) are increasingly understood to play important roles in cell biology, development, and disease, though the vast majority of annotated lncRNAs have yet to be functionally characterized.
Kevin Nyberg, Richard Carthew
doaj   +2 more sources

A recombinase-activated ribozyme to knock down endogenous gene expression in zebrafish. [PDF]

PLoS Genetics
Precise regulation of gene expression is essential to understand a wide range of biological processes. Control over gene expression can be achieved using site-directed recombinases and endonucleases whose efficiency is variable and dependent on the ...
Thomas Juan, Tonatiuh Molina, Lihan Xie, Sofia Papadopoulou, Bárbara Cardoso, Shivam Govind Jha, Didier Y R Stainier   +6 more
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A hammerhead ribozyme selects mechanically stable conformations for catalysis against viral RNA [PDF]

Communications Biology
Ribozymes, widely found in prokaryotes and eukaryotes, target nucleic acids and can be engineered as biotechnical tools or for gene regulation or immune therapy. Among them, hammerhead is the smallest and best characterized ribozyme.
Man Lu, Zhiqiang Cao, Luoan Xiong, Hongying Deng, Kangkang Ma, Ning Liu, Yanding Qin, Shen-Bo Chen, Jun-Hu Chen, Yao Li, Yijin Liu, Zhongbo Yu   +11 more
doaj   +2 more sources

Structural Expansion of Catalytic RNA Nanostructures through Oligomerization of a Cyclic Trimer of Engineered Ribozymes

Molecules, 2023
The multimolecular assembly of three-dimensionally structured proteins forms their quaternary structures, some of which have high geometric symmetry. The size and complexity of protein quaternary structures often increase in a hierarchical manner, with ...
Mst. Ayesha Siddika, Hiroki Oi, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa   +6 more
doaj   +1 more source

RNase P Ribozyme Effectively Inhibits Human CC-Chemokine Receptor 5 Expression and Human Immunodeficiency Virus 1 Infection

Zoonotic Diseases, 2023
Developing novel antiviral agents and approaches is essential for the treatment against human and zoonotic viruses. We had previously produced RNase P-based ribozyme variants capable of efficiently cleaving mRNA in vitro.
Bin Yan, Yujun Liu, Yuan-Chuan Chen, Isadora Zhang, Fenyong Liu   +4 more
doaj   +1 more source

A convolutional neural network for the prediction and forward design of ribozyme-based gene-control elements

eLife, 2021
Ribozyme switches are a class of RNA-encoded genetic switch that support conditional regulation of gene expression across diverse organisms. An improved elucidation of the relationships between sequence, structure, and activity can improve our capacity ...
Calvin M Schmidt, Christina D Smolke
doaj   +1 more source

Effective inhibition of human immunodeficiency virus 1 replication by engineered RNase P ribozyme. [PDF]

PLoS ONE, 2012
Using an in vitro selection procedure, we have previously isolated RNase P ribozyme variants that efficiently cleave an mRNA sequence in vitro. In this study, a variant was used to target the HIV RNA sequence in the tat region.
Wenbo Zeng, Yuan-Chuan Chen, Yong Bai, Phong Trang, Gia-Phong Vu, Sangwei Lu, Jianguo Wu, Fenyong Liu   +7 more
doaj   +1 more source

Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Molecules, 2022
Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization.
Mst. Ayesha Siddika, Takahiro Yamada, Risako Aoyama, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa   +7 more
doaj   +1 more source

A cell-based ribozyme reporter system employing a chromosomally-integrated 5′ exonuclease gene

BMC Molecular and Cell Biology, 2021
Background Bioinformatic genome surveys indicate that self-cleaving ribonucleic acids (ribozymes) appear to be widespread among all domains of life, although the functions of only a small number have been validated by biochemical methods.
Aiyada Aroonsri, Jindaporn Kongsee, Jeremy David Gunawan, Daniel Abidin Aubry, Philip James Shaw   +4 more
doaj   +1 more source