Results 91 to 100 of about 28,198 (287)
Virology Journal, 2010 Background Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester backbone.
Chen Long-Hua +4 more
doaj +1 more source
Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis
PLoS ONE, 2016 CRISPR/Cas9 is now regularly used for targeted mutagenesis in a wide variety of systems. Here we report the use of ribozymes for the generation of gRNAs both in vitro and in zebrafish embryos.
R. Lee, A. Ng, P. Ingham
semanticscholar +1 more source
Advances in Isotope Labeling for Solution Nucleic Acid Nuclear Magnetic Resonance Spectroscopy
ChemPlusChem, EarlyView.Recent advances in the field of nucleic acid nuclear magnetic resonance (NMR) are presented. Stable isotope labeling protocols and new approaches to segmentally label nucleic acid with NMR‐active residues are summarized. The novel methods will have a strong impact on the future of nucleic acid NMR and the characterization of structure and dynamics of ...
Stefan Hilber +3 more
wiley +1 more source
Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC
BioTechniques, 1997 The cleavage activity of synthetic ribozymes needs to be characterized by reliable and rapid methods. A chromatographic method to simultaneously quantitate the amounts of substrate, cleavage fragments and ribozyme is described.
L. Citti +4 more
doaj +1 more source
Biochemistry, 2015 The hammerhead ribozyme is a self-cleaving RNA broadly dispersed across all kingdoms of life. Although it was the first of the small, nucleolytic ribozymes discovered, the mechanism by which it catalyzes its reaction remains elusive.
Aamir Mir +6 more
semanticscholar +1 more source
Electrochemical Science Advances, EarlyView.ABSTRACT Cancer is a result of uncontrolled cell growth with the potential to damage or spread to another part of the body. It is the deadliest disease in the world; therefore, rapid and sensitive detection is essential to fight it. In the past few decades, many diagnosis tools have been developed to detect cancer and monitor therapy progress.
Md Mobarok Karim, Tahera Lasker
wiley +1 more source
RNAiFold 2.0: A web server and software to design custom and Rfam-based RNA molecules [PDF]
, 2015 Several algorithms for RNA inverse folding have been used to design synthetic riboswitches, ribozymes and thermoswitches, whose activity has been experimentally validated. The RNAiFold software is unique among approaches for inverse folding in that (exhaustive) constraint programming is used instead of heuristic methods.
arxiv +1 more source
BMC Research Notes, 2008 Background Hepatitis delta virus (HDV) is a satellite virus of hepatitis B. During viral replication the 1700-nucleotide-long genomic RNA and its complement, the antigenomic RNA, undergo self-cleavage catalyzed by internal ribozyme motifs that are ...
Dobrynina Nadezhda +4 more
doaj +1 more source
Angewandte Chemie, 2015 Nucleolytic ribozymes catalyze site-specific cleavage of their phosphodiester backbones. A minimal version of the twister ribozyme is reported that lacks the phylogenetically conserved stem P1 while retaining wild-type activity.
M. Košutić +12 more
semanticscholar +1 more source
Exploring the Potential and Advancements of Circular RNA Therapeutics
Exploration, EarlyView.Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Lei Wang +6 more
wiley +1 more source