Results 11 to 20 of about 44,034 (296)
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme [PDF]
Nature Communications, 2020 RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate ...
Johan O. L. Andreasson +3 more
doaj +4 more sources
RNase P Ribozymes Inhibit the Replication of Human Cytomegalovirus by Targeting Essential Viral Capsid Proteins [PDF]
Viruses, 2015 An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
Zhu Yang +9 more
doaj +5 more sources
A Faster Triphosphorylation Ribozyme. [PDF]
PLoS ONE, 2015 In support of the RNA world hypothesis, previous studies identified trimetaphosphate (Tmp) as a plausible energy source for RNA world organisms. In one of these studies, catalytic RNAs (ribozymes) that catalyze the triphosphorylation of RNA 5'-hydroxyl ...
Gregory F Dolan +2 more
doaj +7 more sources
Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme
Nature Communications, 2017 Twister-sister is a self-cleaving ribozyme. Here, the authors report the 2.0 Å crystal structure of the four-way junctional twister-sister ribozyme in the pre-catalytic state and discuss mechanistic implications based on their mutagenesis experiments and
Luqian Zheng +7 more
doaj +2 more sources
The Hammerhead Ribozyme: A Long History for a Short RNA
Molecules, 2017 Small nucleolytic ribozymes are a family of naturally occurring RNA motifs that catalyse a self-transesterification reaction in a highly sequence-specific manner.
Marcos de la Peña +2 more
doaj +2 more sources
Ribozyme-catalysed RNA synthesis using triplet building blocks
eLife, 2018 RNA-catalyzed RNA replication is widely believed to have supported a primordial biology. However, RNA catalysis is dependent upon RNA folding, and this yields structures that can block replication of such RNAs.
James Attwater +4 more
doaj +2 more sources
Structure and mechanism of a methyltransferase ribozyme
Nature Chemical Biology, 2022 Known ribozymes in contemporary biology perform a limited range of chemical catalysis, but in vitro selection has generated species that catalyze a broader range of chemistry; yet, there have been few structural and mechanistic studies of selected ...
J. Deng +8 more
semanticscholar +1 more source
An RNA polymerase ribozyme that synthesizes its own ancestor
Proceedings of the National Academy of Sciences of the United States of America, 2020 Significance Life depends on the propagation of heritable information across successive generations. An RNA polymerase ribozyme was obtained by in vitro evolution that has an unprecedented level of activity in copying complex RNA templates.
Katrina F. Tjhung +3 more
semanticscholar +1 more source
Ligand‐dependent ribozymes [PDF]
WIREs RNA, 2016 The discovery of catalytic RNA (ribozymes) more than 30 years ago significantly widened the horizon of RNA‐based functions in natural systems. Similarly to the activity of protein enzymes that are often modulated by the presence of an interaction partner, some examples of naturally occurring ribozymes are influenced by ligands that can either act as ...
Felletti, Michele, Hartig, Jörg S.
openaire +3 more sources
Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]
, 2020 Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P +3 more
core +1 more source