Results 11 to 20 of about 12,837 (178)
Ligand‐dependent ribozymes [PDF]
WIREs RNA, 2016 The discovery of catalytic RNA (ribozymes) more than 30 years ago significantly widened the horizon of RNA‐based functions in natural systems. Similarly to the activity of protein enzymes that are often modulated by the presence of an interaction partner, some examples of naturally occurring ribozymes are influenced by ligands that can either act as ...
Felletti, Michele, Hartig, Jörg S.
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Molecular Therapy: Nucleic Acids, 2021 Hepatocellular carcinoma (HCC) has high fatality rate and limited therapeutic options. Here, we propose a new anti-HCC approach with high cancer-selectivity and efficient anticancer effects, based on adenovirus-mediated Tetrahymena group I trans-splicing
Seung Ryul Han +12 more
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Current Opinion in Structural Biology, 2007 The structural molecular biology of ribozymes took another great leap forward during the past two years. Before ribozymes were discovered in the early 1980s, all enzymes were thought to be proteins. No detailed structural information on ribozymes became available until 1994.
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Viruses, 2014 RNase P ribozyme can be engineered to be a sequence-specific gene-targeting agent with promising application in both basic research and clinical settings.
Zhu Yang +7 more
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Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
Viruses, 2022 The hepatitis delta virus (HDV) genome has an autocatalytic region called the ribozyme, which is essential for viral replication. The aim of this study was to use next-generation sequencing (NGS) to analyze the ribozyme quasispecies (QS) in order to ...
Beatriz Pacin-Ruiz +17 more
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Journal of Investigative Dermatology Symposium Proceedings, 2002 Since their initial discovery, ribozymes have shown great promise not just as a tool in the manipulation of gene expression, but also as a novel therapeutic agent. This review discusses the promises and pitfalls of ribozyme technology, with a special emphasis on cancer-related applications, though relevance to skin disease will also be discussed.
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Biology, 2014 The R3C ligase ribozyme is an artificial ligase ribozyme produced by modification of the ribozyme that lacks cytidine. Here, we attempted to modify the original R3C ribozyme (73 nucleotides) by reducing the number of nucleotides while maintaining the ...
Eri Kurihara +3 more
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Inhibition of human cytomegalovirus immediate early gene expression and growth by a novel RNase P ribozyme variant. [PDF]
PLoS ONE, 2017 We have previously engineered new RNase P-based ribozyme variants with improved in vitro catalytic activity. In this study, we employed a novel engineered variant to target a shared mRNA region of human cytomegalovirus (HCMV) immediate early proteins 1 ...
Xu Sun +13 more
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Fluorescence Polarization for Monitoring Ribozyme Reactions in Real Time
BioTechniques, 2000 Fluorescence polarization has been used recently to monitor diverse macromolecular interactions. In this report, the application of fluorescence polarization has been extended to monitor ribozyme reactions in real time.
K.K. Singh +4 more
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Communications Biology, 2023 Investigating gene function relies on the efficient manipulation of endogenous gene expression. Currently, a limited number of tools are available to robustly manipulate endogenous gene expression between “on” and “off” states. In this study, we insert a
Jie Fang +5 more
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