Results 11 to 20 of about 28,198 (287)

Engineering of Small Ribozymes Acting on RNA: What is Needed to Make a New Function Work with an Existing Catalyst? [PDF]

Chembiochem
Rational design is a potent strategy for developing ribozymes with advanced functionalities. Engineered variants of the hairpin ribozyme impressively demonstrate how function can be tuned through structural manipulation. The engineering of nucleic acids has been a longstanding objective in research, with the field gaining significant attention ...
Ebermann C, Müller S.
europepmc   +2 more sources

A split ribozyme system for in vivo plant RNA imaging and genetic engineering. [PDF]

Plant Biotechnol J
Summary RNA plays a central role in plants, governing various cellular and physiological processes. Monitoring its dynamic abundance provides a discerning understanding of molecular mechanisms underlying plant responses to internal (developmental) and external (environmental) stimuli, paving the way for advances in plant biotechnology to engineer crops
Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X.   +11 more
europepmc   +2 more sources

Charge-density reduction promotes ribozyme activity in RNA–peptide coacervates via RNA fluidization and magnesium partitioning

Nature Chemistry, 2022
It has long been proposed that phase-separated compartments can provide a basis for the formation of cellular precursors in prebiotic environments.
Juan M. Iglesias-Artola, B. Drobot, Mrityunjoy Kar, A. Fritsch, H. Mutschler, T-Y Dora Tang, M. Kreysing   +6 more
semanticscholar   +1 more source

RNase P Ribozyme Effectively Inhibits Human CC-Chemokine Receptor 5 Expression and Human Immunodeficiency Virus 1 Infection

Zoonotic Diseases, 2023
Developing novel antiviral agents and approaches is essential for the treatment against human and zoonotic viruses. We had previously produced RNase P-based ribozyme variants capable of efficiently cleaving mRNA in vitro.
Bin Yan, Yujun Liu, Yuan-Chuan Chen, Isadora Zhang, Fenyong Liu   +4 more
doaj   +1 more source

Structure and mechanism of a methyltransferase ribozyme

Nature Chemical Biology, 2022
Known ribozymes in contemporary biology perform a limited range of chemical catalysis, but in vitro selection has generated species that catalyze a broader range of chemistry; yet, there have been few structural and mechanistic studies of selected ...
J. Deng, T. J. Wilson, Jia Wang, Xuemei Peng, Mengxiao Li, Xiaowei Lin, Wenjian Liao, D. Lilley, Lin Huang   +8 more
semanticscholar   +1 more source

A convolutional neural network for the prediction and forward design of ribozyme-based gene-control elements

eLife, 2021
Ribozyme switches are a class of RNA-encoded genetic switch that support conditional regulation of gene expression across diverse organisms. An improved elucidation of the relationships between sequence, structure, and activity can improve our capacity ...
Calvin M Schmidt, Christina D Smolke
doaj   +1 more source

A cell-based ribozyme reporter system employing a chromosomally-integrated 5′ exonuclease gene

BMC Molecular and Cell Biology, 2021
Background Bioinformatic genome surveys indicate that self-cleaving ribonucleic acids (ribozymes) appear to be widespread among all domains of life, although the functions of only a small number have been validated by biochemical methods.
Aiyada Aroonsri, Jindaporn Kongsee, Jeremy David Gunawan, Daniel Abidin Aubry, Philip James Shaw   +4 more
doaj   +1 more source

Effective inhibition of human immunodeficiency virus 1 replication by engineered RNase P ribozyme. [PDF]

PLoS ONE, 2012
Using an in vitro selection procedure, we have previously isolated RNase P ribozyme variants that efficiently cleave an mRNA sequence in vitro. In this study, a variant was used to target the HIV RNA sequence in the tat region.
Wenbo Zeng, Yuan-Chuan Chen, Yong Bai, Phong Trang, Gia-Phong Vu, Sangwei Lu, Jianguo Wu, Fenyong Liu   +7 more
doaj   +1 more source

An RNA polymerase ribozyme that synthesizes its own ancestor

Proceedings of the National Academy of Sciences of the United States of America, 2020
Significance Life depends on the propagation of heritable information across successive generations. An RNA polymerase ribozyme was obtained by in vitro evolution that has an unprecedented level of activity in copying complex RNA templates.
Katrina F. Tjhung, M. Shokhirev, David P Horning, G. F. Joyce   +3 more
semanticscholar   +1 more source

Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Molecules, 2022
Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization.
Mst. Ayesha Siddika, Takahiro Yamada, Risako Aoyama, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa   +7 more
doaj   +1 more source