Results 31 to 40 of about 28,198 (287)
Nature Communications, 2019 Membraneless compartments, such as complex coacervates, have been hypothesized as plausible prebiotic micro-compartments due to their ability to sequester RNA; however, their compatibility with essential RNA World chemistries is unclear.
Raghav R. Poudyal +5 more
semanticscholar +1 more source
Induction of ribozyme activity by anti-ribozyme oligonucleotides [PDF]
Nucleic Acids Symposium Series, 1999 A new type of hammerhead ribozyme, with cleavage activity enhanced by oligonucleotides, was constructed. Stem II of the ribozyme was substituted with a non complementary loop (loop II). The modified ribozyme exhibited negligible cleavage of a target RNA; however, it was converted to an active molecule in the presence of oligonucleotides which were ...
Nobuhiro Kazama +3 more
openaire +3 more sources
Equilibrium properties and force-driven unfolding pathways of RNA molecules [PDF]
Phys. Rev. Lett. 103: 188102 (2009), 2009 The mechanical unfolding of a simple RNA hairpin and of a 236--bases portion of the Tetrahymena thermophila ribozyme is studied by means of an Ising--like model. Phase diagrams and free energy landscapes are computed exactly and suggest a simple two--state behaviour for the hairpin and the presence of intermediate states for the ribozyme ...
arxiv +1 more source
Journal of Investigative Dermatology Symposium Proceedings, 2002 Since their initial discovery, ribozymes have shown great promise not just as a tool in the manipulation of gene expression, but also as a novel therapeutic agent. This review discusses the promises and pitfalls of ribozyme technology, with a special emphasis on cancer-related applications, though relevance to skin disease will also be discussed.
openaire +2 more sources
Complete RNA inverse folding: computational design of functional hammerhead ribozymes [PDF]
Nucl. Acids Res. (2014) 42 (18): 11752-11762, 2014 Nanotechnology and synthetic biology currently constitute one of the most innovative, interdisciplinary fields of research, poised to radically transform society in the 21st century. This paper concerns the synthetic design of ribonucleic acid molecules, using our recent algorithm, RNAiFold, which can determine all RNA sequences whose minimum free ...
arxiv +1 more source
Biology, 2014 The R3C ligase ribozyme is an artificial ligase ribozyme produced by modification of the ribozyme that lacks cytidine. Here, we attempted to modify the original R3C ribozyme (73 nucleotides) by reducing the number of nucleotides while maintaining the ...
Eri Kurihara +3 more
doaj +1 more source
RNA-Puzzles Round III: 3D RNA structure prediction of five riboswitches and one ribozyme
RNA: A publication of the RNA Society, 2017 RNA-Puzzles is a collective experiment in blind 3D RNA structure prediction. We report here a third round of RNA-Puzzles. Five puzzles, 4, 8, 12, 13, 14, all structures of riboswitch aptamers and puzzle 7, a ribozyme structure, are included in this round
Zhichao Miao +45 more
semanticscholar +1 more source
Viruses, 2015 An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
Zhu Yang +9 more
doaj +1 more source
Hairpin Ribozyme Genes Curtail Alcohol Drinking: from Rational Design to in vivo Effects in the Rat
Molecular Therapy: Nucleic Acids, 2016 Ribozyme genes were designed to reduce voluntary alcohol drinking in a rat model of alcohol dependence. Acetaldehyde generated from alcohol in the liver is metabolized by the mitochondrial aldehyde dehydrogenase (ALDH2) such that diminishing ALDH2 ...
Amalia Sapag +5 more
doaj +1 more source
Inhibition of human cytomegalovirus immediate early gene expression and growth by a novel RNase P ribozyme variant. [PDF]
PLoS ONE, 2017 We have previously engineered new RNase P-based ribozyme variants with improved in vitro catalytic activity. In this study, we employed a novel engineered variant to target a shared mRNA region of human cytomegalovirus (HCMV) immediate early proteins 1 ...
Xu Sun +13 more
doaj +1 more source