Results 41 to 50 of about 44,034 (296)
Growth, Dissolution and Segregation of Genetically Encoded RNA Droplets by Ribozyme Catalysis
Angewandte Chemie, EarlyView.Ribozymes enable sequence‐encoded turnover of RNA droplet material, resulting in transiently active droplets that selectively segregate based on their genotype. This establishes a direct link between genetic information and droplet behavior, marking a step toward programmable, evolvable RNA‐based compartments with life‐like properties.
Franziska Giessler +5 more
wiley +2 more sources
Hairpin Ribozyme Genes Curtail Alcohol Drinking: from Rational Design to in vivo Effects in the Rat
Molecular Therapy: Nucleic Acids, 2016 Ribozyme genes were designed to reduce voluntary alcohol drinking in a rat model of alcohol dependence. Acetaldehyde generated from alcohol in the liver is metabolized by the mitochondrial aldehyde dehydrogenase (ALDH2) such that diminishing ALDH2 ...
Amalia Sapag +5 more
doaj +1 more source
Polyanion-Assisted Ribozyme Catalysis Inside Complex Coacervates.
ACS Chemical Biology, 2019 Owing to their ability to encapsulate biomolecules, complex coacervates formed by associative phase separation of oppositely charged polyelectrolytes have been postulated as prebiotic nonmembranous compartments (NMCs).
Raghav R. Poudyal +2 more
semanticscholar +1 more source
The Kiss Switch Brings Inactive R3C Ligase Ribozyme Back to Life
Biology, 2018 R3C ligase ribozyme catalyzes the nucleophilic attack by a 3′-hydroxyl on a 5′-α-phosphorus of triphosphates to form a 3′-5′-phosphodiester bond. In the present study, although the truncation of R3C ribozyme was accompanied by a large reduction in ...
Kana Tanizawa +4 more
doaj +1 more source
Nature Communications, 2019 The nematode Caenorhabditis elegans represents an important research model. Convenient methods for conditional induction of gene expression in this organism are not available.
Lena A. Wurmthaler +4 more
semanticscholar +1 more source
Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity. [PDF]
, 2010 The ability to generate RNA aptamers for synthetic biology using in vitro selection depends on the informational complexity (IC) needed to specify functional structures that bind target ligands with desired affinities in physiological concentrations of ...
Carothers, James M +4 more
core +2 more sources
Journal of the American Chemical Society, 2019 Molecular evolution can be conceptualized as a walk over a “fitness landscape”, or the function of fitness (e.g., catalytic activity) over the space of all possible sequences.
A. Pressman +7 more
semanticscholar +1 more source
Inhibition of telomerase activity by HDV ribozyme in cancers
Journal of Experimental & Clinical Cancer Research, 2011 Background Telomerase plays an important role in cell proliferation and carcinogenesis and is believed to be a good target for anti-cancer drugs. Elimination of template function of telomerase RNA may repress the telomerase activity.
Gao Yanjing +4 more
doaj +1 more source
Design of Mammalian ON-Riboswitches Based on Tandemly Fused Aptamer and Ribozyme.
ACS Synthetic Biology, 2019 Self-cleaving ribozymes engineered to be activated or inhibited by a small molecule binding to an RNA aptamer inserted within a ribozyme (aptazymes) have proven to be useful for controlling gene expression in living cells. In mammalian cells, an aptazyme
Kamila Mustafina +2 more
semanticscholar +1 more source
Equilibrium properties and force-driven unfolding pathways of RNA molecules
, 2009 The mechanical unfolding of a simple RNA hairpin and of a 236--bases portion of the Tetrahymena thermophila ribozyme is studied by means of an Ising--like model.
A. Imparato +3 more
core +1 more source