Results 51 to 60 of about 12,837 (178)
BMC Cell Biology, 2001 Background Activation of the extracellular signal-regulated kinases ERK1 and ERK2 in hepatocytes by prostaglandin (PG)F2α was recently found to be inhibited by pertussis toxin (PTX) suggesting a role for Gi proteins. Results Targeting the Gi2α expression
Sioud Mouldy +2 more
doaj +1 more source
The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease [PDF]
Iranian Journal of Basic Medical Sciences, 2017 Objective(s): In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD).
Song-Ming Zhuo +4 more
doaj +1 more source
Mini‐Hairpin DNA: Extraordinarily Stable Structure and Its Applications
Chemistry – A European Journal, EarlyView.Mini‐hairpin DNA fragments containing GCGAAGC and GCGAAAGC form exceptionally stable hairpin‐like structures, with only two G−C pairs and one sheared G−A pair, remaining undenatured in 7 M urea and resisting nuclease digestion. These mini‐hairpin sequences frequently appear in replication and transcription origins, influence DNA sequencing fidelity ...
Michiko Kimoto, Ichiro Hirao
wiley +1 more source
Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC
BioTechniques, 1997 The cleavage activity of synthetic ribozymes needs to be characterized by reliable and rapid methods. A chromatographic method to simultaneously quantitate the amounts of substrate, cleavage fragments and ribozyme is described.
L. Citti +4 more
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Rational Design Strategies for Stimuli‐Responsive DNAzymes Using Modified and Artificial Nucleotides
Chemistry – A European Journal, EarlyView.Activity control of RNA‐cleaving DNAzymes in response to external stimuli has attracted growing interest for applications in DNA‐based diagnostics, therapeutics, and dynamic DNA nanotechnology. This review summarizes rational design strategies for stimuli‐responsive DNAzymes using modified or artificial nucleotides, including split and caged DNAzymes ...
Yusuke Takezawa, Mitsuhiko Shionoya
wiley +1 more source
BMC Research Notes, 2008 Background Hepatitis delta virus (HDV) is a satellite virus of hepatitis B. During viral replication the 1700-nucleotide-long genomic RNA and its complement, the antigenomic RNA, undergo self-cleavage catalyzed by internal ribozyme motifs that are ...
Dobrynina Nadezhda +4 more
doaj +1 more source
Yeast, EarlyView.ABSTRACT Given the biotechnological potential of yeast‐derived oils for oleochemical production, genes encoding lipid metabolism enzymes are key targets for metabolic engineering. Genetic engineering tools such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, Transcription Activator‐Like Effector Nucleases (TALENs), Zinc ...
Rodrigo Gonçalves Dias +5 more
wiley +1 more source
Virology Journal, 2010 Background Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester backbone.
Chen Long-Hua +4 more
doaj +1 more source
Genome Biology, 2023 Background CRISPR-Cas13 is a newly emerging RNA knockdown technology that is comparable to RNAi. Among all members of Cas13, CasRx degrades RNA in human cells with high precision and effectiveness.
Yonghao Zhan +4 more
doaj +1 more source
Hammerhead ribozyme kinetics [PDF]
RNA, 1998 The hammerhead ribozyme is a small RNA motif that self cleaves at a specific phosphodiester bond to produce 2′,3′ cyclic phosphate and 5′ hydroxyl termini (Hutchins et al., 1986; Forster & Symons, 1987a). The secondary structure of the hammerhead consists of three helices of arbitrary sequence and length (designated I, II, and III) that intersect at 15
T K, Stage-Zimmermann, O C, Uhlenbeck
openaire +2 more sources