Results 61 to 70 of about 12,837 (178)

SARS‐CoV‐2 nucleocapsid protein variants have differential RNA chaperone activity

The FEBS Journal, EarlyView.
The SARS‐CoV‐2 nucleocapsid (N) protein facilitates RNA annealing through its RBD–IDR2–CTD region, identifying it as a functional RNA chaperone. The Omicron BA.5 variant shows reduced chaperone activity compared to the Wuhan wild‐type protein. Although phosphorylation has no effect on wild‐type N, phosphomimetic modification of BA.5 N restores its RNA ...
Sabrina Babl, Julia M. Seidel, Fabian Kugler, Elisabeth Silberhorn, Anna Ludwig, Jonas Abel, Sophia Winklbauer, Niklas Schaub, Silvia Materna‐Reichelt, Kamran Honarnejad, Nataša Stojanović Gužvić, Gernot Längst   +11 more
wiley   +1 more source

A structural analysis of in vitro catalytic activities of hammerhead ribozymes

BMC Bioinformatics, 2007
Background Ribozymes are small catalytic RNAs that possess the dual functions of sequence-specific RNA recognition and site-specific cleavage. Trans-cleaving ribozymes can inhibit translation of genes at the messenger RNA (mRNA) level in both eukaryotic ...
Schneider Erasmus, Klapper Jessie R, Chan Chi, Wu Susan, Shao Yu, Ding Ye   +5 more
doaj   +1 more source

Protein design and RNA design: Perspectives

Quantitative Biology, Volume 14, Issue 2, June 2026.
Abstract Advances in deep learning and generative modeling have transformed the landscape of protein and RNA design, enabling rapid and precise creation of novel biomolecules with tailored structures and functions. In protein design, generative deep learning frameworks now support backbone generation, sequence optimization, and joint sequence–structure
Xi Chen, Xu Dai, Peilong Lu
wiley   +1 more source

The structure and catalytic mechanism of a pseudoknot-containing hammerhead ribozyme

Nature Communications
We have determined the crystal structure of a pseudoknot (PK)-containing hammerhead ribozyme that closely resembles the pistol ribozyme, with essentially identical secondary structure and connectivity. The activity is more sensitive to deletion of the G8
Xuelin Zhan, Timothy J. Wilson, Zhenzhen Li, Jingjing Zhang, Yili Yang, David M. J. Lilley, Yijin Liu   +6 more
doaj   +1 more source

Programming Next‐Generation Synthetic Biosensors by Genetic Circuit Design

Advanced Science, Volume 13, Issue 14, 9 March 2026.
Synthetic biology enables genetic circuit‐based biosensing to detect diverse targets, process signals, and transduce them into readable outputs or intracellular regulatory activities. However, field deployment and real‐world application of such synthetic biosensors face considerable challenges in sensitivity, specificity, speed, stability, and ...
Yuanli Gao, Cheng Huang, Jiaxuan Deng, Lei Wang, Baojun Wang   +4 more
wiley   +1 more source

Recognition, Signal Transduction, and Amplification of Nucleic Acids Under Isothermal Condition

Advanced Genetics, Volume 7, Issue 1, March 2026.
This Perspective outlines a modular framework for isothermal nucleic acid detection, highlighting recognition and signal transduction as central determinants of assay performance, with amplification strategies serving as sensitivity enhancers. Emerging design principles and future opportunities for scalable and accessible diagnostics are discussed ...
Zhaohui Qin, Jiaxin Li, Xin Su, Huiyu Liu   +3 more
wiley   +1 more source

Therapeutic Gene Editing: DNA Repair Pathways, Emerging Editors, and Clinical Progress

iNew Medicine, Volume 2, Issue 1, March 2026.
ABSTRACT The field of gene editing has evolved rapidly over the past decade, progressing from programmable zinc‐finger nucleases (ZFNs) and transcription activator‐like effector nucleases (TALENs) to the widespread adoption of CRISPR‐Cas systems. First‐generation editors catalyzed genome engineering by introducing targeted double‐strand breaks (DSBs ...
Li‐Kuang Tsai, Youcai Xiong, Dongqi Liu, Dongshan Yang, Jifeng Zhang, Jie Xu, Renzhi Han, Y. Eugene Chen   +7 more
wiley   +1 more source

Ions as Architects of DNA Nanostructures: Mechanisms, Simulations, and Technological Frontiers

Small Structures, Volume 7, Issue 3, March 2026.
Ions are not merely passive electrolytes but active regulators of DNA nanostructures. This review shows how ion valence, hydration, and specificity govern electrostatic screening, correlation, and confinement, enabling DNA origami, metallized DNA, nanopores, hydrogels, DNAzymes, molecular machines, and energy devices through integrated experimental and
Sergiy Perepelytsya, Dmytro Piatnytskyi, Tetiana Bubon, Narcis Cibotariu, Aatto Laaksonen, Francesca Mocci   +5 more
wiley   +1 more source

Ribozyme-catalysed RNA synthesis using triplet building blocks

eLife, 2018
RNA-catalyzed RNA replication is widely believed to have supported a primordial biology. However, RNA catalysis is dependent upon RNA folding, and this yields structures that can block replication of such RNAs.
James Attwater, Aditya Raguram, Alexey S Morgunov, Edoardo Gianni, Philipp Holliger   +4 more
doaj   +1 more source

Temporal Control Over Complex, Simple, and Multiphase Coacervates Using Ureolysis and Ammonium Carbonate Decomposition

ChemSystemsChem, Volume 8, Issue 2, March 2026.
A new strategy for temporal control over simple and complex coacervates is described here. The urea‐urease reaction results in the timed formation and/or destruction of complex coacervates after controllable delays. This strategy is easily adaptable to different time scales and polyelectrolyte pairs.
Shana Shirin Valapra, Malak Jaber, Tsvetomir Ivanov, Lucas Caire da Silva, Guillermo Monreal Santiago   +4 more
wiley   +1 more source