Results 61 to 70 of about 28,198 (287)
Large phenotype jumps in biomolecular evolution [PDF]
Phys. Rev. E 69 (2004) 031908.1-7, 2003 By defining the phenotype of a biopolymer by its active three-dimensional shape, and its genotype by its primary sequence, we propose a model that predicts and characterizes the statistical distribution of a population of biopolymers with a specific phenotype, that originated from a given genotypic sequence by a single mutational event.
arxiv +1 more source
Applied Sciences, 2021 Ribozymes are catalytic RNAs that are attractive platforms for the construction of nanoscale objects with biological functions. We designed a dimeric form of the Tetrahymena group I ribozyme as a unit structure in which two ribozymes were connected in a ...
Junya Akagi +8 more
doaj +1 more source
The Therapeutic Potential of Ribozymes [PDF]
Blood, 1998 AbstractRibozymes are catalytic RNA molecules that recognize their target RNA in a highly sequence-specific manner. They can therefore be used to inhibit deleterious gene expression (by cleavage of the target mRNA) or even repair mutant cellular RNAs. Targets such as the mRNAs of oncogenes (resulting from base mutations or chromosome translocations, eg,
James, H A, Gibson, Ian
openaire +4 more sources
Advanced Engineering Materials, EarlyView.Molecular dynamics simulations are advancing the study of ribonucleic acid (RNA) and RNA‐conjugated molecules. These developments include improvements in force fields, long‐timescale dynamics, and coarse‐grained models, addressing limitations and refining methods.
Kanchan Yadav, Iksoo Jang, Jong Bum Lee
wiley +1 more source
Persistence Length Changes Dramatically as RNA Folds [PDF]
Phys. Rev. Lett 95, 268303 (2005), 2005 We determine the persistence length, $l_p$, for a bacterial group I ribozyme as a function of concentration of monovalent and divalent cations by fitting the distance distribution functions $P(r)$ obtained from small angle X-ray scattering intensity data to the asymptotic form of the calculated $P_{WLC}(r)$ for a worm-like chain (WLC). The $l_p$ values
arxiv +1 more source
Inducible knockdown of Plasmodium gene expression using the glmS ribozyme.
PLoS ONE, 2013 Conventional reverse genetic approaches for study of Plasmodium malaria parasite gene function are limited, or not applicable. Hence, new inducible systems are needed. Here we describe a method to control P.
Parichat Prommana +7 more
doaj +1 more source
Plant Methods, 2022 Background Traditional CRISPR/Cas9 systems that rely on U6 or U3 snRNA promoters (RNA polymerase III-dependent promoters) can only achieve constitutive gene editing in plants, hampering the functional analysis of specifically expressed genes.
Jia-Wen Li +9 more
doaj +1 more source
Witnessing the structural evolution of an RNA enzyme
eLife, 2021 An RNA polymerase ribozyme that has been the subject of extensive directed evolution efforts has attained the ability to synthesize complex functional RNAs, including a full-length copy of its own evolutionary ancestor.
Xavier Portillo +4 more
doaj +1 more source
Deep Sequencing Analysis of Aptazyme Variants Based on a Pistol Ribozyme.
ACS Synthetic Biology, 2017 Chemically regulated self-cleaving ribozymes, or aptazymes, are emerging as a promising class of genetic devices that allow dynamic control of gene expression in synthetic biology.
Shungo Kobori +2 more
semanticscholar +1 more source
Recent Applications of Mesoporous Silica Nanoparticles in Gene Therapy
Advanced Healthcare Materials, EarlyView.The review summarizes the synthesis of mesoporous silica nanoparticles (MSNs) with modifiable surface properties, functionalization strategies, mechanism of therapeutic payload release, and current applications in gene therapy, focusing on their capabilities in the targeted delivery of therapeutic nucleic acids, CRISPR‐Cas systems, and other genetic ...
Tamanna Binte Huq +4 more
wiley +1 more source