Results 71 to 80 of about 43,119 (293)
Witnessing the structural evolution of an RNA enzyme
eLife, 2021 An RNA polymerase ribozyme that has been the subject of extensive directed evolution efforts has attained the ability to synthesize complex functional RNAs, including a full-length copy of its own evolutionary ancestor.
Xavier Portillo +4 more
doaj +1 more source
Advanced Functional Materials, EarlyView.Many known diseases arise from dysregulated gene expression, and differentially expressed genes can serve as biomarkers to distinguish diseased cells from healthy tissues. In this study, reconfigurable nucleic acid nanoparticles (recNANPs) are introduced that can detect overexpressed cancer biomarkers and subsequently release RNAi inducers to silence ...
Yelixza I. Avila +7 more
wiley +1 more source
Processing of insect retrotransposons by self-cleaving ribozymes [PDF]
, 2010 We show that several classes of insect non-LTR retrotransposons harbor self-cleaving ribozymes of the HDV family at their 5′ termini. In Drosophila the R2 ribozymes exhibit highly differential in vivo expression and robust in vitro activity ...
Andrej Luptak +3 more
core +1 more source
Recent Applications of Mesoporous Silica Nanoparticles in Gene Therapy
Advanced Healthcare Materials, EarlyView.The review summarizes the synthesis of mesoporous silica nanoparticles (MSNs) with modifiable surface properties, functionalization strategies, mechanism of therapeutic payload release, and current applications in gene therapy, focusing on their capabilities in the targeted delivery of therapeutic nucleic acids, CRISPR‐Cas systems, and other genetic ...
Tamanna Binte Huq +4 more
wiley +1 more source
Deep Sequencing Analysis of Aptazyme Variants Based on a Pistol Ribozyme.
ACS Synthetic Biology, 2017 Chemically regulated self-cleaving ribozymes, or aptazymes, are emerging as a promising class of genetic devices that allow dynamic control of gene expression in synthetic biology.
Shungo Kobori +2 more
semanticscholar +1 more source
Complex Donuts: Small Variations in DNA Sequence Dictate Pathway Complexity in DNA Nanotoroids
Angewandte Chemie, Volume 137, Issue 33, August 11, 2025.Chemical information encoded in the sequence of DNA amphiphiles enables the selective formation of kinetically trapped DNA nanotoroids through a complex self‐assembly pathway. The toroids are formed as on‐pathway structures via a competitive mechanism only when a toroid‐selective DNA sequence is used.
Muhammad Ghufran Rafique +9 more
wiley +2 more sources
Engineered Cas12j‐8 is a Versatile Platform for Multiplexed Genome Modulation in Mammalian Cells
Advanced Science, EarlyView.Engineered through structure‐guided protein engineering, enCas12j‐8 significantly enhances the editing efficiency of compact Cas12j‐8 while maintaining high specificity. It enables efficient multiplexed genome editing and base editing using a single crRNA array, demonstrating broad applicability and therapeutic potential in genome engineering ...
Ru Meng +14 more
wiley +1 more source
High‐Throughput Mutational Analysis of a Twister Ribozyme
Angewandte Chemie, 2016 Recent discoveries of new classes of self‐cleaving ribozymes in diverse organisms have triggered renewed interest in the chemistry and biology of ribozymes.
Shungo Kobori, Y. Yokobayashi
semanticscholar +1 more source
Angewandte Chemie, Volume 137, Issue 33, August 11, 2025.We show that antisense oligonucleotides can be used to restructure secondary structural elements around the circular DNA template binding sites of the genomic RNA for subsequent rolling circle amplification. This strategy was employed to identify otherwise COVID “false negative” saliva samples by accurately detecting the presence of SARS‐CoV‐2 genomic ...
Amal Mathai +4 more
wiley +2 more sources
Design and Preparation of a Multimeric Self-Cleaving Hammerhead Ribozyme
BioTechniques, 1997 The activity of a ribozyme can be impaired by additional sequences at the 5′ and 3′ termini of the catalytic sequence. To approach this problem, a system was designed that minimizes sequences upstream and downstream from active regions of a hammerhead ...
Juan Ruiz +3 more
doaj +1 more source