Results 71 to 80 of about 44,034 (296)
Witnessing the structural evolution of an RNA enzyme
eLife, 2021 An RNA polymerase ribozyme that has been the subject of extensive directed evolution efforts has attained the ability to synthesize complex functional RNAs, including a full-length copy of its own evolutionary ancestor.
Xavier Portillo +4 more
doaj +1 more source
High‐Throughput Mutational Analysis of a Twister Ribozyme
Angewandte Chemie, 2016 Recent discoveries of new classes of self‐cleaving ribozymes in diverse organisms have triggered renewed interest in the chemistry and biology of ribozymes.
Shungo Kobori, Y. Yokobayashi
semanticscholar +1 more source
SARS‐CoV‐2 nucleocapsid protein variants have differential RNA chaperone activity
The FEBS Journal, EarlyView.The SARS‐CoV‐2 nucleocapsid (N) protein facilitates RNA annealing through its RBD–IDR2–CTD region, identifying it as a functional RNA chaperone. The Omicron BA.5 variant shows reduced chaperone activity compared to the Wuhan wild‐type protein. Although phosphorylation has no effect on wild‐type N, phosphomimetic modification of BA.5 N restores its RNA ...
Sabrina Babl +11 more
wiley +1 more source
Meeting review : ESF workshop on "Impact of nucleic acid chemistry on gene function analysis: antisense, aptamers, ribozymes and RNAi" [PDF]
, 2008 The shortage of functional information compared to the abundance of sequence information characterizes today’s situation in functional genomics. For many years the knock-down of a gene’s product has been the most powerful way of analysing its function ...
Engels, Joachim W. (Prof. Dr.) +3 more
core
Inducible knockdown of Plasmodium gene expression using the glmS ribozyme.
PLoS ONE, 2013 Conventional reverse genetic approaches for study of Plasmodium malaria parasite gene function are limited, or not applicable. Hence, new inducible systems are needed. Here we describe a method to control P.
Parichat Prommana +7 more
doaj +1 more source
Design and Preparation of a Multimeric Self-Cleaving Hammerhead Ribozyme
BioTechniques, 1997 The activity of a ribozyme can be impaired by additional sequences at the 5′ and 3′ termini of the catalytic sequence. To approach this problem, a system was designed that minimizes sequences upstream and downstream from active regions of a hammerhead ...
Juan Ruiz +3 more
doaj +1 more source
Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay
Nucleic Acids Research, 2016 Hammerhead ribozymes are self-cleaving RNA molecules capable of regulating gene expression in living cells. Their cleavage performance is strongly influenced by intra-molecular loop–loop interactions, a feature not readily accessible through modern ...
Simon Ausländer +4 more
semanticscholar +1 more source
RNA catalysis in model protocell vesicles. [PDF]
, 2005 We are engaged in a long-term effort to synthesize chemical systems capable of Darwinian evolution, based on the encapsulation of self-replicating nucleic acids in self-replicating membrane vesicles.
Chen, Irene A +2 more
core +2 more sources
Protein design and RNA design: Perspectives
Quantitative Biology, Volume 14, Issue 2, June 2026.Abstract Advances in deep learning and generative modeling have transformed the landscape of protein and RNA design, enabling rapid and precise creation of novel biomolecules with tailored structures and functions. In protein design, generative deep learning frameworks now support backbone generation, sequence optimization, and joint sequence–structure
Xi Chen, Xu Dai, Peilong Lu
wiley +1 more source
eLifeA self-cleaving ribozyme that maps to an intron of the cytoplasmic polyadenylation element-binding protein 3 (Cpeb3) gene is thought to play a role in human episodic memory, but the underlying mechanisms mediating this effect are not known. We tested the
Claire C Chen +11 more
doaj +1 more source