Results 71 to 80 of about 28,198 (287)
Functional identification of catalytic metal ion binding sites within RNA. [PDF]
PLoS Biology, 2005 The viability of living systems depends inextricably on enzymes that catalyze phosphoryl transfer reactions. For many enzymes in this class, including several ribozymes, divalent metal ions serve as obligate cofactors.
James L Hougland +3 more
doaj +2 more sources
Molecular Therapy: Nucleic Acids, 2021 Hepatocellular carcinoma (HCC) has high fatality rate and limited therapeutic options. Here, we propose a new anti-HCC approach with high cancer-selectivity and efficient anticancer effects, based on adenovirus-mediated Tetrahymena group I trans-splicing
Seung Ryul Han +12 more
doaj
High‐Throughput Mutational Analysis of a Twister Ribozyme
Angewandte Chemie, 2016 Recent discoveries of new classes of self‐cleaving ribozymes in diverse organisms have triggered renewed interest in the chemistry and biology of ribozymes.
Shungo Kobori, Y. Yokobayashi
semanticscholar +1 more source
A Programmed DNA Dynamic Assembly‐Guided Molecular Amplifier for Authentic Information Decryption
Advanced Science, EarlyView.This study presents a DNA‐based constitutional dynamic network (CDN) with adaptive thresholds for secure information decryption. The system self‐calibrates output patterns, ensuring reproducibility and accuracy, and integrates molecular amplifiers for expanded message capacity and precise DNA sensing.
Ning Yang +9 more
wiley +1 more source
Journal of the American Chemical Society, 2016 We present results from molecular dynamics simulations and free energy calculations of the twister ribozyme at different stages along the reaction path to gain insight into its mechanism. The results, together with recent biochemical experiments, provide
Colin S Gaines, D. York
semanticscholar +1 more source
Design and Preparation of a Multimeric Self-Cleaving Hammerhead Ribozyme
BioTechniques, 1997 The activity of a ribozyme can be impaired by additional sequences at the 5′ and 3′ termini of the catalytic sequence. To approach this problem, a system was designed that minimizes sequences upstream and downstream from active regions of a hammerhead ...
Juan Ruiz +3 more
doaj +1 more source
Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay
Nucleic Acids Research, 2016 Hammerhead ribozymes are self-cleaving RNA molecules capable of regulating gene expression in living cells. Their cleavage performance is strongly influenced by intra-molecular loop–loop interactions, a feature not readily accessible through modern ...
Simon Ausländer +4 more
semanticscholar +1 more source
Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit
Biology, 2023 The class I ligase ribozyme consists of 121 nucleotides and shows a high catalytic rate comparable to that found in natural proteinaceous polymerases. In this study, we aimed to identify the smaller active unit of the class I ligase ribozyme comprising ...
Miho Kasuga +3 more
doaj +1 more source
Journal of the American Chemical Society, 2016 Group II introns are Mg(2+)-dependent ribozymes that are considered to be the evolutionary ancestors of the eukaryotic spliceosome, thus representing an ideal model system to understand the mechanism of conversion of premature messenger RNA (mRNA) into ...
L. Casalino +3 more
semanticscholar +1 more source
CPEB3 is associated with human episodic memory
Frontiers in Behavioral Neuroscience, 2009 Cytoplasmic polyadenylation element-binding (CPEB) proteins are crucial for synaptic plasticity and memory in model organisms. A highly conserved, mammalian-specific short intronic sequence within CPEB3 has been identified as a ribozyme with self ...
Christian Vogler +14 more
doaj +1 more source