Results 81 to 90 of about 44,034 (296)

CPEB3 is associated with human episodic memory

Frontiers in Behavioral Neuroscience, 2009
Cytoplasmic polyadenylation element-binding (CPEB) proteins are crucial for synaptic plasticity and memory in model organisms. A highly conserved, mammalian-specific short intronic sequence within CPEB3 has been identified as a ribozyme with self ...
Christian Vogler, Christian Vogler, Klara Spalek, Klara Spalek, Amanda Aerni, Philippe Demougin, Philippe Demougin, Ariane Müller, Ariane Müller, Kim-Dung Huynh, Kim-Dung Huynh, Andreas Papassotiropoul, Andreas Papassotiropoul, Dominique J F De Quervain, Dominique J F De Quervain   +14 more
doaj   +1 more source

Molecular Crowding‐Driven RNA Condensates Enable Förster Resonance Energy Transfer‐Enhanced Small‐Molecule Sensing

Aggregate, Volume 7, Issue 2, February 2026.
This study presents a dynamic‐responsive sensing system composed of RNA aptamers and polymers for adaptive histamine sensing. Programmable RNA self‐assembly via PEG8K enables phase‐separated condensates with enhanced stability and affinity. A label‐free FRET system (ThT→HBC dyes) achieves high‐sensitive detection (15.36 nM) in complex matrices ...
Yifan Wu, Xuan Ma, Yanger Liu, Zhaoxuan Zhang, Yangzi Zhang, Wenqiang Zhang, Longjiao Zhu, Wentao Xu   +7 more
wiley   +1 more source

Evidence for the involvement of G_i2 in activation of extracellular signal-regulated kinases in hepatocytes

BMC Cell Biology, 2001
Background Activation of the extracellular signal-regulated kinases ERK1 and ERK2 in hepatocytes by prostaglandin (PG)F2α was recently found to be inhibited by pertussis toxin (PTX) suggesting a role for Gi proteins. Results Targeting the Gi2α expression
Sioud Mouldy, Christoffersen Thoralf, Melien Øyvind   +2 more
doaj   +1 more source

Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis

PLoS ONE, 2016
CRISPR/Cas9 is now regularly used for targeted mutagenesis in a wide variety of systems. Here we report the use of ribozymes for the generation of gRNAs both in vitro and in zebrafish embryos.
R. Lee, A. Ng, P. Ingham
semanticscholar   +1 more source

Construction of Reverse Genetics System for Feline Calicivirus FCV‐BJ616 and Proteomic Analysis

MicrobiologyOpen, Volume 15, Issue 1, February 2026.
This study successfully isolated the FCV‐BJ616 strain derived from cats and established a reverse genetics system. In vivo experiments showed that rFCV‐BJ616 exhibits wild‐type virulence, leading to high fever, weight loss, and multi‐organ lesions in infected cats. Proteomic analysis indicated that both rFCV‐BJ616 and FCV‐BJ616 share similar regulatory
Chunmei Xu, Jingjie Zhao, Hao Liu, Haotian Gu, Xinming Tang, Lin Liang, Jiabo Ding, Shaohua Hou, Xiaomin Zhao, Ruiying Liang   +9 more
wiley   +1 more source

Rescue of Synthetic Genomic RNA Analogs of Rabies Virus by Plasmid-Encoded Proteins [PDF]

, 1994
Proteins eolirely expressed from cDNA wen used to rescue synthetic RNA genome analogs into infectious defective particles or rabies virus (RV). Synthetic negative-stranded RNAs coßtalning 3' · and S'-terminal RV sequences and tnlßscriptional ...
Conzelmann, Karl-Klaus, Schnell, M.-J.
core   +3 more sources

Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC

BioTechniques, 1997
The cleavage activity of synthetic ribozymes needs to be characterized by reliable and rapid methods. A chromatographic method to simultaneously quantitate the amounts of substrate, cleavage fragments and ribozyme is described.
L. Citti, L. Boldrini, S. Nevischi, L. Mariani, G. Rainaldi   +4 more
doaj   +1 more source

The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease [PDF]

Iranian Journal of Basic Medical Sciences, 2017
Objective(s): In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD).
Song-Ming Zhuo, Si-Cong Li, Yong-Qun Lin, Hai-Bin Yu, Na Li   +4 more
doaj   +1 more source

Crystal structure of a tRNA acceptor‐stem mimic at 1.94 Å resolution

Acta Crystallographica Section F, Volume 82, Issue 2, Page 57-65, February 2026.
This paper reports the structure solution of an RNA complex using anomalous scattering.Despite the theoretical advantages of phosphorus single‐wavelength anomalous diffraction (P‐SAD) for nucleic acid phasing, its application remains limited due to high atomic displacement parameters and an unfavourable ratio of unique reflections to anomalous ...
Ziwei Liu, Dom Bellini, Fabrice Gorrec, Armin Wagner, Kamel El Omari, John D. Sutherland   +5 more
wiley   +1 more source

Selection of chromosomal DNA libraries using a multiplex CRISPR system. [PDF]

, 2014
The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that ...
Arkin, Adam P, Cate, Jamie HD, DeLoache, Will, Dueber, John E, Lee, Michael E, Li, Xin, Maurer, Matthew J, Poddar, Snigdha, Ryan, Owen W, Skerker, Jeffrey M, Tsai, Jordan C   +10 more
core   +2 more sources