SensorsEfficient bacterial lysis and RNA purification are essential for molecular diagnostics and biosensing applications. This study presents a piezoelectric platform integrated with gold-plated RNA capture pins (RCPs) functionalized with synthetic ...
Md Aminul Islam +2 more
doaj +1 more source
Novel Peptide Conjugates of Modified Oligonucleotides for Inhibition of Bacterial RNase P
Frontiers in Pharmacology, 2019 Novel alternatives to traditional antibiotics are now of great demand for the successful treatment of microbial infections. Here, we present the engineering and properties of new oligonucleotide inhibitors of RNase P, an essential bacterial enzyme.
Darya Novopashina +17 more
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From high throughput 454 GS FLX data analysis process of 16S RNA gene sequences using barcoding to bacterial community exploration [PDF]
, 2011 From high throughput 454 GS FLX data analysis process of 16S RNA gene sequences using barcoding to bacterial community ...
Cauquil, Laurent +7 more
core
Global Transcriptomic Profiling of Bovine Endometrial Immune Response In Vitro. II. Effect of Bovine Viral Diarrhea Virus on the Endometrial Response to Lipopolysaccharide [PDF]
, 2015 Infection with noncytopathic bovine viral diarrhea virus (ncpBVDV) is associated with uterine disease and infertility. This study investigated the influence of ncpBVDV on immune functions of the bovine endometrium by testing the response to bacterial ...
Abudureyimu, A +6 more
core +1 more source
Nature CommunicationsStaphylococcus aureus is a major human pathogen responsible for severe infections. While traditionally described as extracellular, increasing evidence establishes S. aureus as a facultative intracellular pathogen.
Ines Rodrigues Lopes +7 more
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Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival. [PDF]
, 2017 Salmonella have developed a sophisticated machinery to evade immune clearance and promote survival in the infected cells. Previous studies were mostly focused on either bacteria itself or host cells, the interaction mechanism of host-pathogen awaits ...
Chen, Xi +11 more
core +2 more sources
DNA recognition by an RNA-guided bacterial Argonaute
PLoS ONE, 2017 Argonaute (Ago) proteins are widespread in prokaryotes and eukaryotes and share a four-domain architecture capable of RNA- or DNA-guided nucleic acid recognition. Previous studies identified a prokaryotic Argonaute protein from the eubacterium Marinitoga
K. Doxzen, J. Doudna
semanticscholar +1 more source
Accurately assembling nanopore sequencing data of highly pathogenic bacteria
BMC GenomicsBackground Bacterial genome exploration and outbreak analysis rely heavily on robust whole-genome sequencing and bioinformatics analysis. Widely-used genomic methods, such as genotyping and detection of genetic markers demand high sequencing accuracy and
Christine Thomas +5 more
doaj +1 more source
Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies [PDF]
, 2018 In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for ...
Angelidaki, Irini +4 more
core +3 more sources
Frontiers in Microbiology, 2014 Analysis of microbial gene expression during host colonisation provides valuable information on the nature of interaction, beneficial or pathogenic, and the adaptive processes involved.
Ashleigh eHolmes +3 more
doaj +1 more source