Structural insights into RNA processing by the human RISC-loading complex. [PDF]
, 2009 Targeted gene silencing by RNA interference (RNAi) requires loading of a short guide RNA (small interfering RNA (siRNA) or microRNA (miRNA)) onto an Argonaute protein to form the functional center of an RNA-induced silencing complex (RISC).
AD Haase +41 more
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Quantitative analysis of competition in post-transcriptional regulation reveals a novel signature in target expression variation [PDF]
, 2013 When small RNAs are loaded onto Argonaute proteins they can form the RNA-induced silencing complexes (RISCs), which mediate RNA interference. RISC-formation is dependent on a shared pool of Argonaute proteins and RISC loading factors, and is thus ...
Berg, Johannes, Klironomos, Filippos D.
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High-Resolution Structure of the N-Terminal Endonuclease Domain of the Lassa Virus L Polymerase in Complex with Magnesium Ions [PDF]
, 2014 Lassa virus (LASV) causes deadly hemorrhagic fever disease for which there are no vaccines and limited treatments. LASV-encoded L polymerase is required for viral RNA replication and transcription.
Dong, Changjiang +6 more
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Structural and biochemical insights into small RNA 3' end trimming by Arabidopsis SDN1. [PDF]
, 2018 A family of DEDDh 3'→5' exonucleases known as Small RNA Degrading Nucleases (SDNs) initiates the turnover of ARGONAUTE1 (AGO1)-bound microRNAs in Arabidopsis by trimming their 3' ends. Here, we report the crystal structure of Arabidopsis SDN1 (residues 2-
Cao, Chunyang +10 more
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The Exosome Subunit Rrp44 Plays a Direct Role in RNA Substrate Recognition [PDF]
, 2007 The exosome plays key roles in RNA maturation and surveillance, but it is unclear how target RNAs are identified. We report the functional characterization of the yeast exosome component Rrp44, a member of the RNase II family.
Anderson, James T. +2 more
core +2 more sources
Expression of the C-terminal family 22 carbohydratebinding module of xylanase 10B of Clostridium themocellum in tobacco plant [PDF]
, 2009 Carbohydrate-binding modules have been shown to alter plant cell wall structural architecture. Hence, they have the potential application of being used to engineer the plant to produce tailor-made natural fibers in the cell wall.
Obembe, Olawole O.
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RNase P Ribozymes Inhibit the Replication of Human Cytomegalovirus by Targeting Essential Viral Capsid Proteins. [PDF]
, 2015 An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
LIU, Fenyong +9 more
core +3 more sources
Transient tether between the SRP RNA and SRP receptor ensures efficient cargo delivery during cotranslational protein targeting [PDF]
, 2010 Kinetic control of macromolecular interactions plays key roles in biological regulation. An example of such control occurs in cotranslational protein targeting by the signal recognition particle (SRP), during which the SRP RNA and the cargo both ...
Shan, Shu-ou, Shen, Kuang
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The telomerase essential N-terminal domain promotes DNA synthesis by stabilizing short RNA-DNA hybrids. [PDF]
, 2015 Telomerase is an enzyme that adds repetitive DNA sequences to the ends of chromosomes and consists of two main subunits: the telomerase reverse transcriptase (TERT) protein and an associated telomerase RNA (TER). The telomerase essential N-terminal (TEN)
Akiyama, Benjamin M +2 more
core +2 more sources
The origins and physical roots of life’s dual – metabolic and genetic – nature [PDF]
, 2017 This review paper aims at a better understanding of the origin and physical foundation of life’s dual – metabolic and genetic – nature. First, I give a concise ‘top-down’ survey of the origin of life, i.e., backwards in time from extant DNA/RNA/protein ...
De Tiège, Alexis
core +2 more sources