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Recent Advances in Non-Viral Vectors for Gene Therapy and Gene Delivery: From Lipid Nanoparticles to Engineered Extracellular Vesicles. [PDF]

Pharmaceutics
Yang Y, Song T, Huang K, Huang H, Zhao M, Li Y.   +5 more
europepmc   +1 more source

CRISPRing through time: How cutting-edge technology is revolutionizing life sciences and medicine. [PDF]

Mol Ther Nucleic Acids
Banik I, Coppé JP.
europepmc   +1 more source

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Get ready for the CRISPR/Cas system: A beginner's guide to the engineering and design of guide RNAs

The Journal of Gene Medicine, 2021
AbstractThe clustered regularly interspaced short palindromic repeats (CRISPR) system is a state‐of‐the‐art tool for versatile genome editing that has advanced basic research dramatically, with great potential for clinic applications. The system consists of two key molecules: a CRISPR‐associated (Cas) effector nuclease and a single guide RNA.
Shufeng Ma, Jie Lv, Zinan Feng, Zhili Rong, Ying Lin   +4 more
openaire   +2 more sources

Nucleic Acid Detection through RNA‐Guided Protease Activity in Type III‐E CRISPR‐Cas Systems

ChemBioChem, 2023
AbstractRNA‐guided protease activity was recently discovered in the type III‐E CRISPR‐Cas systems (Craspase), providing a novel platform for engineering a protein probe instead of the commonly used nucleic acid probe in nucleic acid detection assays.
Qiuqiu He, Xinlong Lei, Yuanjun Liu, Xiaoshen Wang, Nan Ji, Hang Yin, Huiping Wang, Heng Zhang, Guimei Yu   +8 more
openaire   +2 more sources

Advances in guide RNA design for editing plant genomes using CRISPR-Cas systems

2021
This chapter discusses the general rules for selecting target sites for genome editing using the CRISPR-Cas technology and summarizes the bioinformatic tools that can be used to design sgRNA sequences.
Kaiyuan Chen, Hao Liu, Kabin Xie, Muhammad Tahir ul Qamar, Ling-Ling Chen   +4 more
openaire   +1 more source

Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration

Nature, 2019
Conventional CRISPR-Cas systems maintain genomic integrity by leveraging guide RNAs for the nuclease-dependent degradation of mobile genetic elements, including plasmids and viruses. Here we describe a notable inversion of this paradigm, in which bacterial Tn7-like transposons have co-opted nuclease-deficient CRISPR-Cas systems to catalyse RNA-guided ...
Sanne E, Klompe, Phuc L H, Vo, Tyler S, Halpin-Healy, Samuel H, Sternberg   +3 more
openaire   +2 more sources

Reconstitution and biochemical characterization of the RNA-guided helicase-nuclease protein Cas3 from type I-A CRISPR–Cas system

2022
Type I is the most prevalent CRISPR system found in nature. It can be further defined into six subtypes, from I-A to I-G. Among them, the Type I-A CRISPR-Cas systems are almost exclusively found in hyperthermophilic archaeal organisms. The system achieves RNA-guided DNA degradation through the concerted action of a CRISPR RNA containing complex Cascade
Chunyi, Hu, Ailong, Ke
openaire   +2 more sources