Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]
PLoS ONE, 2015 Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt +8 more
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Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene [PDF]
BMC Bioinformatics, 2018 Background Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between ...
Francesco Sambo +9 more
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16S ribosomal RNA gene-based metagenomics: A review
Biomedical Research Journal, 2020 With the advent of contemporary molecular tools, the conventional microbiological isolation, enrichment techniques, and approaches have changed considerably.
Asmita Kamble +2 more
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Experience and Insight Into Genetic Diagnosis of Infective Aortic Aneurysm [PDF]
Clinical Case ReportsCulture‐negative infected aneurysms possibly occur in various clinical situations, including prior antibiotic exposure. Accurate microbial identification is crucial for an optimal antimicrobial strategy.
Shinnosuke Fukushima +3 more
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Transcriptomic detection of Candidatus Allocryptoplasma (Anaplasmataceae) in Galápagos marine iguanas (Amblyrhynchus cristatus, Iguanidae) [PDF]
Parasites & VectorsBackground Globally, the disease ecology of reptiles remains understudied, even for threatened and iconic species such as the Galápagos marine iguana (Amblyrhynchus cristatus).
Flora Uesseler +9 more
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A systematic algorithm using 16S ribosomal RNA for accurate diagnosis of pneumonia pathogens [PDF]
Scientific ReportsOver half of community-acquired pneumonia cases are caused by a few dozen bacterial species, and accurate identification of these pathogens is essential for effective treatment. In this study, we developed a reliable diagnostic method using 16S ribosomal
Ferry Dwi Kurniawan +5 more
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Computer modeling 16 S ribosomal RNA [PDF]
Journal of Molecular Biology, 1991 A three-dimensional structure for 16 S RNA has been produced with a computer protocol that is not dependent on human intervention. This protocol improves upon traditional modeling techniques by using distance geometry to fold the molecule in an objective and reproducible fashion.
J M, Hubbard, J E, Hearst
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Systematic identification of gene families for use as markers for phylogenetic and phylogeny- driven ecological studies of bacteria and archaea and their major subgroups [PDF]
, 2013 With the astonishing rate that the genomic and metagenomic sequence data sets are accumulating, there are many reasons to constrain the data analyses.
Eisen, Jonathan A. +2 more
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Frontiers in Microbiology, 2022 Ribosome assembly is a complex fundamental cellular process that involves assembling multiple ribosomal proteins and several ribosomal RNA species in a highly coordinated yet flexible and resilient manner.
Vignesh M. P. Babu +3 more
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A comparison of structural and evolutionary attributes of Escherichia coli and Thermus thermophilus small ribosomal subunits: signatures of thermal adaptation. [PDF]
PLoS ONE, 2013 Here we compare the structural and evolutionary attributes of Thermus thermophilus and Escherichia coli small ribosomal subunits (SSU). Our results indicate that with few exceptions, thermophilic 16S ribosomal RNA (16S rRNA) is densely packed compared to
Saurav Mallik, Sudip Kundu
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