Results 221 to 230 of about 549,357 (248)

Selective nuclear export mechanism of small RNAs

Current Opinion in Structural Biology, 2011
The timely nuclear-cytoplasmic translocation of proteins and RNAs by importins and exportins is important for controlling biological processes. Since the 2004 publication of the first exportin structure, Cse1p, the X-ray structures of exportin-5 complexed with pre-microRNA, exportin-t complexed with tRNA, and three CRM1-related structures have revealed
Soo Jae, Lee, Chimari, Jiko, Eiki, Yamashita, Tomitake, Tsukihara   +3 more
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Pseudourine formation in small nuclear RNAs

Biochimie, 1994
Recent in vitro studies on the formation of pseudouridine (psi) in the spliceosomal small nuclear RNAs (snRNA) are reviewed. Multiple psi synthase activities, in some cases more that one per snRNA, are responsible for this modification of uridine. There is a requirement for Sm protein binding for the efficient formation of psi in U5 RNA but not for the
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Non-coding RNAs: lessons from the small nuclear and small nucleolar RNAs

Nature Reviews Molecular Cell Biology, 2007
Recent advances have fuelled rapid growth in our appreciation of the tremendous number, diversity and biological importance of non-coding (nc)RNAs. Because ncRNAs typically function as ribonucleoprotein (RNP) complexes and not as naked RNAs, understanding their biogenesis is crucial to comprehending their regulation and function.
A Gregory, Matera, Rebecca M, Terns, Michael P, Terns   +2 more
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Nuclear Export of Small RNAs

Science, 2009
The machinery that exports small RNAs from the cell nucleus is fine-tuned to recognize when the processing of each class of RNA has been completed.
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Isolation of Small Nuclear RNAs

1994
For isolation of small nuclear (sn)RNAs from maize leaf tissue, we use a slightly modified procedure described originally by Kiss et al. (1985) for dicot plants. This procedure consists of two basic steps: (1) purification of maize nuclei and (2) extraction of snRNAs.
Tamás Kiss, Witold Filipowicz
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Synthesis of Small Nuclear RNAs

1991
There are seven abundant and several less abundant capped small nuclear RNAs characterized in mammalian cells. These RNAs are all capped on their 5′ ends and were designated U snRNAs because the U1-U3 snRNAs initially studied were rich in uridylic acid (Hodnett and Busch 1968).
R. Reddy, R. Singh
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Small nuclear RNAs in messenger RNA and ribosomal RNA processing.

The FASEB Journal, 1993
Ribonucleoproteins (RNPs) play essential roles in many aspects of gene expression. Two families of nuclear RNPs are involved in the processing of primary transcripts made by RNA polymerases I and II (pol I and II), two of the three polymerases present in the nuclei of eukaryotic cells.
I W, Mattaj, D, Tollervey, B, Séraphin
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The role of small nuclear RNAs in RNA splicing

Current Opinion in Cell Biology, 1993
Recent genetic and biochemical experiments have revealed an intimate and dynamic role for small nuclear RNAs (snRNAs) in multiple steps of RNA-splicing reactions. Both snRNA-substrate and snRNA-snRNA interactions are involved. These interactions concern not only splice site and branch point definition, but also the catalytic reactions of the first and ...
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Biosynthesis of small nuclear RNAs in human cells

Journal of Cellular Physiology, 1983
AbstractWe have examined some aspects of the biosynthesis of human small nuclear RNAs (snRNAs). The sensitivity of U5 and U4 snRNA synthesis to α‐amanitin in whole cells suggests that RNA polymerase II is involved in the synthesis of these RNA species, in addition to that of U1, U2, and U3 snRNA.
S C, Chandrasekharappa, J H, Smith, G L, Eliceiri   +2 more
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Small nuclear RNAs and pre-mRNA splicing

Current Opinion in Cell Biology, 1994
Genetic and biochemical studies have recently shown that the splice sites in nuclear pre-mRNAs are aligned for the transesterification reactions through a collaboration between U5 and U6 small nuclear RNAs, which form the heart of a network of RNA-based interactions in the active spliceosome.
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