Results 221 to 230 of about 573,592 (269)

Pseudourine formation in small nuclear RNAs

Biochimie, 1994
Recent in vitro studies on the formation of pseudouridine (psi) in the spliceosomal small nuclear RNAs (snRNA) are reviewed. Multiple psi synthase activities, in some cases more that one per snRNA, are responsible for this modification of uridine. There is a requirement for Sm protein binding for the efficient formation of psi in U5 RNA but not for the
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Synthesis of Small Nuclear RNAs

1991
There are seven abundant and several less abundant capped small nuclear RNAs characterized in mammalian cells. These RNAs are all capped on their 5′ ends and were designated U snRNAs because the U1-U3 snRNAs initially studied were rich in uridylic acid (Hodnett and Busch 1968).
R. Reddy, R. Singh
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Isolation of Small Nuclear RNAs

1994
For isolation of small nuclear (sn)RNAs from maize leaf tissue, we use a slightly modified procedure described originally by Kiss et al. (1985) for dicot plants. This procedure consists of two basic steps: (1) purification of maize nuclei and (2) extraction of snRNAs.
Tamás Kiss, Witold Filipowicz
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Genes for human U4 small nuclear RNA

Gene, 1986
A study of human genes coding for U4 small nuclear RNA is presented. It is known from previous studies that mammalian cells contain three major U4 RNA species, designated U4A, U4B, and U4C (Krol and Branlant, 1981). A clone was isolated from a human DNA library which contained two transcriptionally active genes for U4 RNA.
C, Bark, P, Weller, J, Zabielski, U, Pettersson   +3 more
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Nuclear Export of Small RNAs

Science, 2009
The machinery that exports small RNAs from the cell nucleus is fine-tuned to recognize when the processing of each class of RNA has been completed.
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Selective nuclear export mechanism of small RNAs

Current Opinion in Structural Biology, 2011
The timely nuclear-cytoplasmic translocation of proteins and RNAs by importins and exportins is important for controlling biological processes. Since the 2004 publication of the first exportin structure, Cse1p, the X-ray structures of exportin-5 complexed with pre-microRNA, exportin-t complexed with tRNA, and three CRM1-related structures have revealed
Soo Jae, Lee, Chimari, Jiko, Eiki, Yamashita, Tomitake, Tsukihara   +3 more
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Associations of small nuclear RNAs in human cells

Journal of Cellular Physiology, 1983
AbstractSeveral observations have been made about the associations of small nuclear RNAs (snRNAs) in human cells. When nuclear RNA was extracted with phenol and chloroform under standard nondenaturing conditions, the proportion of the nuclear snRNA content that cosedimented with high molecular weight RNA was very low.
J H, Smith, G L, Eliceiri
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Small molecular weight monodisperse nuclear RNA

Journal of Molecular Biology, 1968
Abstract The nuceloplasm and nucleolus of HeLa cells contain six distinct low molecular weight species of RNA. Their size, as estimated from electrophoretic mobility, ranges from 100 to 180 nucleotides. These RNA species are long-lived and do not appear to be precursor to any cytoplasmic product.
R A, Weinberg, S, Penman
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Small nuclear RNAs in messenger RNA and ribosomal RNA processing.

The FASEB Journal, 1993
Ribonucleoproteins (RNPs) play essential roles in many aspects of gene expression. Two families of nuclear RNPs are involved in the processing of primary transcripts made by RNA polymerases I and II (pol I and II), two of the three polymerases present in the nuclei of eukaryotic cells.
I W, Mattaj, D, Tollervey, B, Séraphin
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Formation of Pseudouridine in U5 Small Nuclear RNA

Biochemistry, 1994
The formation of pseudouridine (psi) on U5 small nuclear RNA (U5 snRNA) was studied using an in vitro modification system. Labeled U5 RNA, synthesized in vitro and therefore unmodified, was incubated in reactions containing S100 and/or nuclear extracts (NE) from HeLa cells, and the levels of psi were determined.
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