Results 231 to 240 of about 549,357 (248)

Gene therapy with modified U1 small nuclear RNA

Expert Review of Endocrinology & Metabolism, 2017
More than 15% of all disease-causing mutations result in mRNA splicing defects. U1 snRNA binds to the 5' splice site (5'ss) through base pairing. Mutation-adapted U1 snRNA (with compensatory U1 snRNA changes) and exon-specific U1 snRNA (complementary to intronic sequences) have been shown to suppress 5'ss mutations in cellular and animal models.
Wuh-Liang, Hwu, Yu-May, Lee, Ni-Chung, Lee   +2 more
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Metabolism of small molecular weight monodisperse nuclear RNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
Abstract The small molecular weight, nuclear monodisperse RNA (snRNA) species of HeLa cells have been further characterized. 1. 1. In addition to the six species previously reported, three additional ones are now described. 2. 2. Several monodisperse, metabolically unstable RNA species are found in the cytoplasmic supernatant.
R, Weinberg, S, Penman
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Close Structural Resemblance among Small Nuclear RNAs

The Journal of Biochemistry, 1983
The published primary and secondary structures of small nuclear RNAs (snRNAs) (1-9) were reexamined and a new common model for their secondary structures was proposed. What is surprising is the resemblance of their secondary structures. U1, U2, U4, and U5 snRNAs share a common sequence of A(U)nG (n greater than or equal to 3) at the single-stranded ...
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Associations of small nuclear RNAs in human cells

Journal of Cellular Physiology, 1983
AbstractSeveral observations have been made about the associations of small nuclear RNAs (snRNAs) in human cells. When nuclear RNA was extracted with phenol and chloroform under standard nondenaturing conditions, the proportion of the nuclear snRNA content that cosedimented with high molecular weight RNA was very low.
J H, Smith, G L, Eliceiri
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Small Nuclear RNAs: RNA Sequences, Structure, and Modifications

1988
All eukaryotic cells contain multiple small nuclear RNAs, designated U-sn At present ten U-snRNAs have been identified; they account for about 1% total mammalian cellular RNA. Of these, the U1 to U8 snRNAs have been sively characterized. All the U-snRNAs contain a 5′-cap structure consis a blocked 5′-terminal pyrophosphate linkage; except for U6 RNA, a
Ram Reddy, Harris Busch
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The status of small nuclear RNA in the ribonucleoprotein fibrils containing heterogeneous nuclear RNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
hnRNP are made of two classes of unit, monoparticles and heterogeneous complexes. The monoparticles are much more easily dissociated by salt than the heterogeneous complexes. We made use of this differential salt sensitivity to determine the localization of snRNA in hnRNP.
H, Gallinaro, M, Jacob
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Formation of Pseudouridine in U5 Small Nuclear RNA

Biochemistry, 1994
The formation of pseudouridine (psi) on U5 small nuclear RNA (U5 snRNA) was studied using an in vitro modification system. Labeled U5 RNA, synthesized in vitro and therefore unmodified, was incubated in reactions containing S100 and/or nuclear extracts (NE) from HeLa cells, and the levels of psi were determined.
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Purification of small nuclear ribonucleoprotein particles with antibodies against modified nucleosides of small nuclear RNAs

1990
Publisher Summary The snRNAs exist in the cell as ribonucleoprotein (RNP) complexes. While the snRNAs U1, U2, and U5 are organized in separate RNP particles, the majority of the snRNAs U4 and U6 reside in a single RNP complex. Several proteins in the molecular weight range of 25K to 200K were recently identified as possible U5-specific proteins ...
M, Bach, P, Bringmann, R, Lührmann
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Small nuclear RNA-protein complexes

Biochemical Society Transactions, 1988
J, Hamm, D, Scherly, I W, Mattaj
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Nuclear RNA splicing and small nuclear RNAs and their genes in higher plants

1991
Abstract This review is focused on the splicing of nuclear precursors of messenger and transfer RNAs, and on the synthesis and structure of the U small nuclear RNAs (U- snRNAs), which participate in various RNA processing events in the nucleus.
Gregory J Goodall, Kiss Tamas, Filipowicz Witold   +2 more
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