Results 41 to 50 of about 1,861,609 (310)

Inhibition of RNA polymerase II transcription in human cells by synthetic DNA-binding ligands [PDF]

, 1998
Sequence-specific DNA-binding small molecules that can permeate human cells potentially could regulate transcription of specific genes. Multiple cellular DNA-binding transcription factors are required by HIV type 1 for RNA synthesis.
Baird, Eldon E., Dervan, Peter B., Dickinson, Liliane A., Gottesfeld, Joel M., Gulizia, Richard J., Mosier, Donald E., Trauger, John W.   +6 more
core   +1 more source

Cataloguing and Selection of mRNAs Localized to Dendrites in Neurons and Regulated by RNA-Binding Proteins in RNA Granules

Biomolecules, 2020
Spatiotemporal translational regulation plays a key role in determining cell fate and function. Specifically, in neurons, local translation in dendrites is essential for synaptic plasticity and long-term memory formation.
Rie Ohashi, Nobuyuki Shiina
doaj   +1 more source

CircRNAs and RNA-Binding Proteins Involved in the Pathogenesis of Cancers or Central Nervous System Disorders

Non-Coding RNA, 2023
Circular RNAs (circRNAs), a newly recognized group of noncoding RNA transcripts, have established widespread attention due to their regulatory role in cell signaling. They are covalently closed noncoding RNAs that form a loop, and are typically generated
Yuka Ikeda, Sae Morikawa, Moeka Nakashima, Sayuri Yoshikawa, Kurumi Taniguchi, Haruka Sawamura, Naoko Suga, Ai Tsuji, Satoru Matsuda   +8 more
doaj   +1 more source

Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera [PDF]

, 2013
DEAD box helicases catalyze the ATP-dependent destabilization of RNA duplexes. Whereas duplex separation is mediated by the helicase core shared by all members of the family, flanking domains often contribute to binding of the RNA substrate.
Klostermeier, Dagmar, Linden, Martin H., Rudolph, Markus G., Steimer, Lenz, Wurm, Jan Philip, Wöhnert, Jens   +5 more
core   +1 more source

FLASH: ultra-fast protocol to identify RNA-protein interactions in cells

, 2019
Determination of the in vivo binding sites of RNA-binding proteins (RBPs) is paramount to understanding their function and how they affect different aspects of gene regulation.
Akhtar, A., Aktas, T., Backofen, R., Ilik, I., Maticzka, D.   +4 more
core   +1 more source

Structural basis for dual roles of Aar2p in U5 snRNP assembly [PDF]

, 2013
Yeast U5 small nuclear ribonucleoprotein particle (snRNP) is assembled via a cytoplasmic precursor that contains the U5-specific Prp8 protein but lacks the U5-specific Brr2 helicase.
Beggs, J.D., Cristão, V.F., Heroven, A.C., Holton, N., Jovin, S.M., Lührmann, R., Santos, K.F., Wahl, M.C., Weber, G.   +8 more
core   +1 more source

RNA Detection in Live Bacterial Cells Using Fluorescent Protein Complementation Triggered by Interaction of Two RNA Aptamers with Two RNA-Binding Peptides

Pharmaceuticals, 2011
Many genetic and infectious diseases can be targeted at the RNA level as RNA is more accessible than DNA. We seek to develop new approaches for detection and tracking RNA in live cells, which is necessary for RNA-based diagnostics and therapy.
Charles R. Cantor, Natalia E. Broude, Paul Toran, Hung-Wei Yiu, Vadim V. Demidov   +4 more
doaj   +1 more source

Protocol to measure protein-RNA binding using double filter-binding assays followed by phosphorimaging or high-throughput sequencing

STAR Protocols, 2023
Summary: Binding affinity quantitatively describes the strength of a molecular interaction and is reported by the equilibrium dissociation constant (KD).
Joel Vega-Badillo, Phillip D. Zamore, Karina Jouravleva   +2 more
doaj   +1 more source

Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba [PDF]

, 2013
Archaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly ...
Ingledew, W John, Jelinska, Clare, Petrovic-Stojanovska, Biljana, White, Malcolm F   +3 more
core   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source