tRNATrp as primer for RNA-directed DNA polymerase: structural determinants of function. [PDF]
Journal of Biological Chemistry, 1979 The specific interactions between the RNA-directed DNA polymerase of avian oncornavirus and the tRNATrp primer required for initiation of viral DNA synthesis in vitro were examined. Two distinct interactions, stable binding of the tRNATrp to the enzyme and initiation of viral DNA synthesis by the enzyme with tRNATrp as primer, were characterized as to ...
Barbara Cordell +3 more
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RNA-directed DNA polymerase from particles released by normal goose cells [PDF]
Journal of Virology, 1979 Cells from a goose embryo were shown to release particle-associated RNA-directed DNA polymerase and RNase H activities that required the presence of Nonidet P-40 for detection. The particles were not infectious and did not have endogenous DNA synthesis.
Georg Bauer, Howard M. Temin
semanticscholar +6 more sources
Characterization of an RNA-directed DNA polymerase found in association with murine intracytoplasmic A-particles [PDF]
Journal of Virology, 1977 An RNA-directed DNA polymerase was found to be associated with intracytoplasmic A-particles from DBA/2 mouse leukemia cells. The enzyme activity was detected after disrupting the purified particles with 2 M NaCl-20 mM dithiothreitol. The presence of a divalent cation and all four deoxyribonucleoside triphosphates was essential for this enzyme activity.
Michiaki Kohno, Hajime Tanaka
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RNA-directed DNA polymerase activity of reticuloendotheliosis virus: characterization of the endogenous and exogenous reactions [PDF]
Journal of Virology, 1975 Reticuloendotheliosis virus (REV) contains an endogenously instructed, RNA-directed DNA polymerase activity. Both the endogenous and exogenous DNA polymerase activities exhibited up to 10-fold greater activity at the optimum concentration of manganous ion (0.025 mM for exogenous; 0.25 mM for endogenous) than at any concentration of magnesium ion ...
Marilynn R. F. Waite, Patton T. Allen
semanticscholar +4 more sources
Polymerase IV occupancy at RNA-directed DNA methylation sites requires SHH1 [PDF]
Nature, 2013 DNA methylation is an epigenetic modification that plays critical roles in gene silencing, development, and genome integrity. In Arabidopsis, DNA methylation is established by DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2) and targeted by 24 nt small interfering RNAs (siRNAs) through a pathway termed RNA-directed DNA methylation (RdDM)1.
Julie A. Law +8 more
semanticscholar +6 more sources
Endogenous RNA-directed DNA polymerase activity in uninfected chicken embryos. [PDF]
Proceedings of the National Academy of Sciences, 1972 Early chicken embryos that are either positive or negative for group-specific antigens of avian leukosis viruses contained endogenous RNA-directed DNA polymerase activity. This endogenous DNA polymerase activity was not increased after mixture of soluble
C. Kang, H. Temin
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Inhibition of RNA-directed DNA polymerase by aurintricarboxylic acid.
Nucleic Acids Research, 1976 Commercial-grade aurintricarboxylic acid (ATA) inhibits poly(A), poly(C) and viral RNA-directed DNA synthesis by detergent-disrupted virions of Moloney murine leukemia virus. Paper chromatography of crude ATA yields two active components, which appear to
J. F. Givens, K. Manly
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Stimulated normal human lymphocytes contain a ribonuclease-sensitive DNA polymerase distinct from viral RNA-directed DNA polymerase. [PDF]
Proceedings of the National Academy of Sciences, 1972 Ribonuclease-sensitive DNA synthesis is demonstrated in a cytoplasmic particulate fraction of normal human blood lymphocytes stimulated with phytohemagglutinin, but not in unstimulated lymphocytes.
Bobrow Sn +3 more
semanticscholar +4 more sources
In Vitro Transcription of 70 S RNA by the RNA-Directed DNA Polymerase of Rous Sarcoma Virus: Lack of Influence of RNase H [PDF]
Journal of Virology, 1976 Marc S. Collett, Anthony J. Faras
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DNA polymerase of reticuloendotheliosis virus: Inability to detect endogenous RNA-directed DNA synthesis [PDF]
Virology, 1975 Abstract Purified preparations of reticuloendotheliosis virus (REV) appear to lack the endogenous RNase-sensitive DNA polymerase activity present in most, if not all, avian RNA tumor viruses. Although no endogenous RNA-directed DNA polymerase could be detected in REV, we have been able to demonstrate the presence of a virion-associated DNA polymerase
Rita M. Kieras, Anthony J. Faras
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