Results 251 to 260 of about 167,761 (271)

Genetic variants in RNA-induced silencing complex genes and prostate cancer

World Journal of Urology, 2017
The purpose of this study is to evaluate the potential association between genetic variants in genes encoding the components of RNA-induced silencing complex and prostate cancer (PCa) risk. Genetic variants chosen for this study are rs3742330 in DICER1, rs4961280 in AGO2, rs784567 in TARBP2, rs7813 in GEMIN4 and rs197414 in GEMIN3.The study involved ...
Z. Nikolić, D. Pavićević, N. Vučić, S. Cerovic, V. Vukotić, G. Brajušković   +5 more
semanticscholar   +4 more sources

miRNA*: a passenger stranded in RNA-induced silencing complex?

Critical Reviews™ in Eukaryotic Gene Expression, 2010
Processing of the pre-microRNA (pre-miRNA) through Dicer1 generates a miRNA duplex, consisting of a miRNA and miRNA* strand (also termed guide strand and passenger strand, respectively). Despite the general consensus that miRNA*s have no regulatory activity, recent publications have provided evidence that the abundance, possible function, and ...
Sarah M. Mah, C. Buske, R. Humphries, Florian Kuchenbauer   +3 more
semanticscholar   +4 more sources

Functional regulation of RNA-induced silencing complex by photoreactive oligonucleotides.

Bioorganic & Medicinal Chemistry, 2014
We developed a novel method for regulation of RISC function by photoreactive oligonucleotides (Ps-Oligo) containing 2'-O-psoralenylmethoxyethyl adenosine (Aps). We observed that inhibitory effects of Ps-Oligos on RISC function were enhanced by UV-irradiation compared with 2'-O-methyl-oligonucleotide without Aps. These results suggest Ps-Oligo inhibited
Yohei Matsuyama, A. Yamayoshi, A. Kobori, A. Murakami   +3 more
semanticscholar   +4 more sources

The Interactomics of the RNA-Induced Silencing Complex

, 2018
The posttranscriptional gene silencing mechanism initially mistaken as co-suppression but later identified as RNA interference mediated and regulated by small interfering RNAs (siRNAs), and microRNAs (miRNAs) has gradually emerged as a landmark discovery of the last decade.
A. Datta, S. Ganguli
semanticscholar   +3 more sources

RNA-Induced Silencing Complex-Bound Small Interfering RNA Is a Determinant of RNA Interference-Mediated Gene Silencing in Mice

Molecular Pharmacology, 2011
Deeper knowledge of pharmacokinetic and pharmacodynamic (PK/PD) concepts for RNA therapeutics is important to streamline the drug development process and for rigorous selection of best performing drug candidates. Here we characterized the PK/PD relationship for small interfering RNAs (siRNAs) targeting luciferase by examining siRNA concentration in ...
Jie Wei, Jeffrey J. Jones, Jing Kang, A. Card, Michael Krimm, Paula J. Hancock, Y. Pei, B. Ason, Elmer Payson, N. Dubinina, M. Cancilla, M. Stroh, J. Burchard, A. Sachs, J. Hochman, W. Flanagan, N. Kuklin, Rna Delivery   +17 more
semanticscholar   +4 more sources

Induction and biochemical purification of RNA-induced silencing complex from Drosophila S2 cells.

Methods in molecular biology, 2004
The discovery of RNA interference (RNAi) has greatly simplified the process of suppressing genes in many experimental systems, including Caenorhabditis elegans, Drosophila, and mammalian cells. A sequence-specific nuclease complex, called the RNA-induced silencing complex (RISC), can be purified from cells undergoing RNAi.
A. Caudy, G. Hannon
semanticscholar   +4 more sources

The microRNA effector RNA-induced silencing complex in hidradenitis suppurativa: a significant dysregulation within active inflammatory lesions

Archives of Dermatological Research, 2017
Recently, we could show that the expression levels of the key regulators of the microRNA (miRNA) maturation and transport were dysregulated in inflamed hidradenitis suppurativa (HS) tissue (Heyam et al. in Wiley Interdiscip Rev RNA 6:271-289, 2015). The RNA-induced silencing complex (RISC) is the central element of the miRNA pathway and regulates miRNA
S. Hessam, Michael Sand, M. Skrygan, F. Bechara   +3 more
semanticscholar   +4 more sources

Rational optimization of siRNA to ensure strand bias in the interaction with the RNA-induced silencing complex

Chemical Communications, 2023
A new class of 5′-morpholino modified nucleoside building blocks was synthesized. Among them, Mo2 exhibited better off-target mitigation for RNAi compared to other analogues Pip and Mo3 and the previously reported morpholino derivative Mo1.
Dhrubajyoti Datta, Christopher S. Theile, Kelly Wassarman, June Qin, Tim Racie, Karyn Schmidt, Yongfeng Jiang, Rachel Sigel, Maja M. Janas, Martin Egli, Muthiah Manoharan   +10 more
openaire   +2 more sources

Immunohistochemical analysis of RNA‐induced silencing complex‐related proteins AGO2 and TNRC6A in prostate and esophageal cancers

APMIS, 2010
Yoo NJ, Hur SY, Kim MS, Lee JY, Lee SH. Immunohistochemical analysis of RNA‐induced silencing complex‐related proteins AGO2 and TNRC6A in prostate and esophageal cancers. APMIS 2010; 118: 271–6.Evidence exists that microRNA (miRNA), which regulates gene expression, is frequently deregulated in cancers.
N. Yoo, Soon-Young Hur, M. S. Kim, Ji Youl Lee, S. Lee   +4 more
semanticscholar   +4 more sources

MiR-21 is Enriched in the RNA-Induced Silencing Complex and Targets COL4A1 in Human Granulosa Cell Lines

Reproductive Sciences, 2012
MicroRNAs (miRNAs) are noncoding small RNAs that play important roles in a variety of physiological and pathological events. In this study, we performed large-scale profiling of EIF2C2-bound miRNAs in 3 human granulosa-derived cell lines (ie, KGN, HSOGT, and GC1a) by high-throughput sequencing and found that miR-21 accounted for more than 80% of EIF2C2-
Yuri Mase, O. Ishibashi, Tomoko Ishikawa, T. Takizawa, K. Kiguchi, T. Ohba, H. Katabuchi, T. Takeshita, T. Takizawa   +8 more
semanticscholar   +4 more sources