Results 281 to 290 of about 284,360 (314)
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Chemical Communications, 2023
A new class of 5′-morpholino modified nucleoside building blocks was synthesized. Among them, Mo2 exhibited better off-target mitigation for RNAi compared to other analogues Pip and Mo3 and the previously reported morpholino derivative Mo1.
Dhrubajyoti Datta +10 more
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A new class of 5′-morpholino modified nucleoside building blocks was synthesized. Among them, Mo2 exhibited better off-target mitigation for RNAi compared to other analogues Pip and Mo3 and the previously reported morpholino derivative Mo1.
Dhrubajyoti Datta +10 more
openaire +2 more sources
The Interactomics of the RNA-Induced Silencing Complex
2018The posttranscriptional gene silencing mechanism initially mistaken as co-suppression but later identified as RNA interference mediated and regulated by small interfering RNAs (siRNAs), and microRNAs (miRNAs) has gradually emerged as a landmark discovery of the last decade.
Sayak Ganguli, Abhijit Datta
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Molecular Pharmacology, 2011
Deeper knowledge of pharmacokinetic and pharmacodynamic (PK/PD) concepts for RNA therapeutics is important to streamline the drug development process and for rigorous selection of best performing drug candidates. Here we characterized the PK/PD relationship for small interfering RNAs (siRNAs) targeting luciferase by examining siRNA concentration in ...
Ananda Card +16 more
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Deeper knowledge of pharmacokinetic and pharmacodynamic (PK/PD) concepts for RNA therapeutics is important to streamline the drug development process and for rigorous selection of best performing drug candidates. Here we characterized the PK/PD relationship for small interfering RNAs (siRNAs) targeting luciferase by examining siRNA concentration in ...
Ananda Card +16 more
openaire +3 more sources
Induction and Biochemical Purification of RNA-Induced Silencing Complex From Drosophila S2 Cells
2004The discovery of RNA interference (RNAi) has greatly simplified the process of suppressing genes in many experimental systems, including Caenorhabditis elegans, Drosophila, and mammalian cells. A sequence-specific nuclease complex, called the RNA-induced silencing complex (RISC), can be purified from cells undergoing RNAi.
Amy A. Caudy, Gregory J. Hannon
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Cancer Research, 2011
Abstract Objectives: There is virtually no treatment for advanced hepatocellular carcinoma (HCC). As such novel targets need to be identified to develop effective therapies to combat this fatal disease. The oncogene Astrocyte elevated gene-1 (AEG-1) is overexpressed in more than 90% of human HCC patients and plays a seminal role in ...
Dong Chen +6 more
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Abstract Objectives: There is virtually no treatment for advanced hepatocellular carcinoma (HCC). As such novel targets need to be identified to develop effective therapies to combat this fatal disease. The oncogene Astrocyte elevated gene-1 (AEG-1) is overexpressed in more than 90% of human HCC patients and plays a seminal role in ...
Dong Chen +6 more
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Reproductive Sciences, 2012
MicroRNAs (miRNAs) are noncoding small RNAs that play important roles in a variety of physiological and pathological events. In this study, we performed large-scale profiling of EIF2C2-bound miRNAs in 3 human granulosa-derived cell lines (ie, KGN, HSOGT, and GC1a) by high-throughput sequencing and found that miR-21 accounted for more than 80% of EIF2C2-
Toshihiro Takizawa +8 more
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MicroRNAs (miRNAs) are noncoding small RNAs that play important roles in a variety of physiological and pathological events. In this study, we performed large-scale profiling of EIF2C2-bound miRNAs in 3 human granulosa-derived cell lines (ie, KGN, HSOGT, and GC1a) by high-throughput sequencing and found that miR-21 accounted for more than 80% of EIF2C2-
Toshihiro Takizawa +8 more
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APMIS, 2010
Yoo NJ, Hur SY, Kim MS, Lee JY, Lee SH. Immunohistochemical analysis of RNA‐induced silencing complex‐related proteins AGO2 and TNRC6A in prostate and esophageal cancers. APMIS 2010; 118: 271–6.Evidence exists that microRNA (miRNA), which regulates gene expression, is frequently deregulated in cancers.
Soo Young Hur +4 more
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Yoo NJ, Hur SY, Kim MS, Lee JY, Lee SH. Immunohistochemical analysis of RNA‐induced silencing complex‐related proteins AGO2 and TNRC6A in prostate and esophageal cancers. APMIS 2010; 118: 271–6.Evidence exists that microRNA (miRNA), which regulates gene expression, is frequently deregulated in cancers.
Soo Young Hur +4 more
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2017
Small RNA-mediated gene silencing is involved in a variety of biological processes among many eukaryotic organisms. The silencing effector, generally referred to as RNA-induced silencing complex (RISC), comprises an ARGONAUTE (AGO) protein and a small single-stranded guide RNA in its core. RISCs recognize target genes containing sequences complementary
Masayuki Ishikawa +2 more
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Small RNA-mediated gene silencing is involved in a variety of biological processes among many eukaryotic organisms. The silencing effector, generally referred to as RNA-induced silencing complex (RISC), comprises an ARGONAUTE (AGO) protein and a small single-stranded guide RNA in its core. RISCs recognize target genes containing sequences complementary
Masayuki Ishikawa +2 more
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Archives of Dermatological Research, 2017
Recently, we could show that the expression levels of the key regulators of the microRNA (miRNA) maturation and transport were dysregulated in inflamed hidradenitis suppurativa (HS) tissue (Heyam et al. in Wiley Interdiscip Rev RNA 6:271-289, 2015). The RNA-induced silencing complex (RISC) is the central element of the miRNA pathway and regulates miRNA
Michael Sand +3 more
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Recently, we could show that the expression levels of the key regulators of the microRNA (miRNA) maturation and transport were dysregulated in inflamed hidradenitis suppurativa (HS) tissue (Heyam et al. in Wiley Interdiscip Rev RNA 6:271-289, 2015). The RNA-induced silencing complex (RISC) is the central element of the miRNA pathway and regulates miRNA
Michael Sand +3 more
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Nucleic Acid Therapeutics, 2017
MicroRNAs (miRNAs) regulate gene expression by forming RNA-induced silencing complexes (RISCs) and have been considered as promising therapeutic targets. MiRNA is an essential component of RISC for the modulation of gene expression. Therefore, the release of miRNA from RISC is considered as an effective method for the inhibition of miRNA functions.
Hiroshi Sugiyama +5 more
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MicroRNAs (miRNAs) regulate gene expression by forming RNA-induced silencing complexes (RISCs) and have been considered as promising therapeutic targets. MiRNA is an essential component of RISC for the modulation of gene expression. Therefore, the release of miRNA from RISC is considered as an effective method for the inhibition of miRNA functions.
Hiroshi Sugiyama +5 more
openaire +3 more sources