Results 91 to 100 of about 28,766 (215)

Purification of human U6 small nuclear RNA capping enzyme. Evidence for a common capping enzyme for gamma-monomethyl-capped small RNAs.

Journal of Biological Chemistry, 1994
To understand the mechanism of gamma-monomethyl (meppp) cap formation, we attempted to identify and purify the U6 small nuclear RNA capping enzyme. Although more than one protein was cross-linked to U6, 7SK, B2, or plant U3 RNA, only one protein of approximately 130 kDa was common to all four meppp-capped RNAs; 5 S RNA, which is an uncapped RNA, was ...
S, Shimba, R, Reddy
openaire   +2 more sources

siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA

Advanced Healthcare Materials, EarlyView.
Osteogenic‐angiogenic cross‐talk is a vital prerequisite for vascularized bone regeneration. In this study, we investigated the effects of siRNA‐mediated silencing of two inhibitory proteins, Chordin and WWP‐1, via CaP‐NP‐loaded gelatin microparticles in osteogenically differentiated microtissues.
Franziska Mitrach, Jonas Kubat, Stefan Simm, Alexandra H. Springwald, Burak Demir, Anton Liebezeit, Michael C. Hacker, Michaela Schulz‐Siegmund   +7 more
wiley   +1 more source

The Nucleoprotein and Phosphoprotein of Measles Virus

Frontiers in Microbiology, 2019
Measles virus is a negative strand virus and the genomic and antigenomic RNA binds to the nucleoprotein (N), assembling into a helical nucleocapsid. The polymerase complex comprises two proteins, the Large protein (L), that both polymerizes RNA and caps ...
Serafima Guseva, Sigrid Milles, Martin Blackledge, Rob W. H. Ruigrok   +3 more
doaj   +1 more source

Engineered ETS1‐Nanoconjugate Restores Immune Homeostasis through Dual Immune‐Vascular Modulation in Relapsing and Progressive Multiple Sclerosis

Advanced Healthcare Materials, EarlyView.
The biomimetic nanoplatform IMNP (ETS1 pDNA/PBAE@ITP‐MM) undergoes targeted disassembly at inflammatory vascular sites to release the ETS1 plasmid (pETS1). This release initiates a cascade of effects that inhibit pathogenic pathways and support immune homeostasis. (Abbreviations: EndMT, endothelial‐to‐mesenchymal transition; EC, endothelial cell; TC, T
Feng Zhang, Si‐Bo Yang, Yan Luo, Xiao‐Di Sun, Bo‐Hao Chang, Da‐Qiang Zhou, Jun‐Jie Jiang, Ya‐Nan Li, Jie‐Hong Wu, Hui‐Juan Jin, Hang Yang, Hang Hu, Yi‐Fan Zhou, Bo Hu   +13 more
wiley   +1 more source

Sprayable Polymer Blends With Short‐Chain Surface Segregation for Preventing Postoperative Abdominal Adhesions

Advanced Healthcare Materials, EarlyView.
Adhesions’ high occurrence rates and high morbidity render them a critical challenge to be addressed. Current prevention methods, such as physical barriers, have many limitations, resulting in inconsistent safety and efficacy. This study demonstrates the potential for sprayable polymeric materials as an adhesion barrier.
Robert J. Morris III, Tejaswi Nori, Alex I. Halpern, Hannah LaPadula, Arthur V. Cresce, Sarah L. Wright, Anthony D. Sandler, Peter Kofinas   +7 more
wiley   +1 more source

An RNA Metabolism and Surveillance Quartet in the Major Histocompatibility Complex

Cells, 2019
At the central region of the mammalian major histocompatibility complex (MHC) is a complement gene cluster that codes for constituents of complement C3 convertases (C2, factor B and C4).
Danlei Zhou, Michalea Lai, Aiqin Luo, Chack-Yung Yu   +3 more
doaj   +1 more source

Nanotherapies for Atherosclerosis: Targeting, Catalysis, and Energy Transduction

Advanced Healthcare Materials, EarlyView.
Atherosclerosis management is hindered by poor drug targeting and plaque heterogeneity. Nanotechnology overcomes these barriers via three core strategies: (1) target‐engineered nanocarriers that achieve lesion‐specific precision via ligand modification, biomimetic camouflage, stimuli‐responsive release, and self‐propelling nanomotors; (2) catalytic ...
Yuqi Yang, Jingguo Qu, Tonghuan Xiao, Xinyang Han, Michael Tin‐Cheung Ying   +4 more
wiley   +1 more source

Mitochondrial RNA capping: highly efficient 5’-RNA capping with NAD⁺ and NADH by yeast and human mitochondrial RNA polymerase

, 2018
AbstractBacterial and eukaryotic nuclear RNA polymerases (RNAPs) cap RNA with the oxidized and reduced forms of the metabolic effector nicotinamide adenine dinucleotide, NAD+ and NADH, using NAD+ and NADH as non-canonical initiating nucleotides for transcription initiation.
Bird, Jeremy G., Basu, Urmimala, Kuster, David, Ramachandran, Aparna, Grudzien-Nogalska, Ewa, Kiledjian, Megerditch, Temiakov, Dmitry, Patel, Smita S., Ebright, Richard H., Nickels, Bryce E.   +9 more
openaire   +1 more source

Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives

Advanced Materials, EarlyView.
Kelvin probe force microscopy (KPFM) enables the nanoscale mapping of electrostatic surface potentials. While widely applied in materials science, its use in biological systems remains emerging. This review presents recent advances in KPFM applied to biological samples and provides a critical perspective on current limitations and future directions for
Ehsan Rahimi, Mario Palacios‐Corella, Arjan Mol, Salvador Pané, Josep Puigmartí‐Luis   +4 more
wiley   +1 more source

5′-cap RNA/SAM mimetic conjugates as bisubstrate inhibitors of viral RNA cap 2′-O-methyltransferases

Bioorganic Chemistry
Viral RNA cap 2'-O-methyltransferases are considered promising therapeutic targets for antiviral treatments, as they play a key role in the formation of viral RNA cap-1 structures to escape the host immune system. A better understanding of how they interact with their natural substrates (RNA and the methyl donor SAM) would enable the rational ...
Ahmed‐belkacem, Rostom, Sutto-Ortiz, Priscila, Delpal, Adrien, Troussier, Joris, Canard, Bruno, Vasseur, Jean-Jacques, Decroly, Etienne, Debart, Françoise   +7 more
openaire   +4 more sources