Results 111 to 120 of about 29,994 (285)

Emerging Roles of m7G-Cap Hypermethylation and Nuclear Cap-Binding Proteins in Bypassing Suppression of eIF4E-Dependent Translation

Viruses
Translation regulation is essential to the survival of hosts. Most translation initiation falls under the control of the mTOR pathway, which regulates protein production from mono-methyl-guanosine (m7G) cap mRNAs.
Kathleen Boris-Lawrie, Jessica Liebau, Abdullgadir Hayir, Xiao Heng   +3 more
doaj   +1 more source

Coupled RNA polymerase II transcription and 3' end formation with yeast whole-cell extracts

, 2010
RNA polymerase II (RNAP II) transcription and pre-mRNA 3' end formation are linked through physical and functional interactions. We describe here a highly efficient yeast in vitro system that reproduces both transcription and 3' end formation in a single
Mariconti, L, Schlinkmann, K., Schlinkmann, Karola, Mariconti, L., Wengi, A., Wengi, A, Bernhard Dichtl, Dichtl, B., Loll, B, Bernhard Loll, Loll, Bernhard, Mariconti, Luisa, Dichtl, Bernhard, Anton Meinhart, Wengi, Agnieszka, Dichtl, B, Meinhart, A, Luisa Mariconti, Schlinkmann, K, Agnieszka Wengi, Loll, B., Karola Schlinkmann, Meinhart, Anton, Meinhart, A.   +23 more
core   +1 more source

Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives

Advanced Materials, EarlyView.
Kelvin probe force microscopy (KPFM) enables the nanoscale mapping of electrostatic surface potentials. While widely applied in materials science, its use in biological systems remains emerging. This review presents recent advances in KPFM applied to biological samples and provides a critical perspective on current limitations and future directions for
Ehsan Rahimi, Mario Palacios‐Corella, Arjan Mol, Salvador Pané, Josep Puigmartí‐Luis   +4 more
wiley   +1 more source

Seroprevalence of Hepatitis C and factors associated with this in crack users

Revista Latino-Americana de Enfermagem, 2013
OBJECTIVE: to investigate the seroprevalence of hepatitis C in crack users in Piauí. METHOD: seroepidemiological survey, undertaken in the Psycho-Social Care Centers for Drugs and Alcohol (CAPS AD) in Piauí in the period December 2011 to May 2012.
Laís Carvalho de Sá, Telma Maria Evangelista de Araújo, Rosane Harter Griep, Viriato Campelo, Claudete Ferreira de Souza Monteiro   +4 more
doaj   +1 more source

A Dual‐Bioresponsive and Programmable Microneedle Matrix as a Bioinspired Coupler for Orchestrating Diabetic Bone Regeneration

Advanced Materials, EarlyView.
This project developed a smart bandage‐like patch (a microneedle array) for repairing diabetic bone damage. It intelligently senses signals from infection and inflammation, then releases its medicines in a specific, timed sequence: first an antibacterial agent, then an anti‐inflammatory agent, and finally growth factors.
Yu Wang, Rui Chen, Yu Chen, Haowen Lu, Yuchen Qian, Zhiheng Gao, Zheming Shen, Xiaofei Zheng, Huilin Yang, Jun Zou, Yusen Qiao   +10 more
wiley   +1 more source

Detection of the DNA and RNA to the transformants.

, 2014
(A) HYG marker was used in the DNA detection. In addition, CAPS marker was used to confirm the positive transformants precisely and the restriction enzyme cutting site (BamHI) lied in the premature stopped position of Pid3-J1; (B) Pid3D marker was used ...
Qiming Lv (544614), Qian Xu (119888), Xiao Xu (112147), Jichen Xu (544619), Zhuangzhi Zhou (544617), Lihuang Zhu (456727), Yong Tao (17333), Guanghuai Jiang (544618), Shigui Li (143517), Xianfeng Zhao (310968), Junjun Shang (544615), Xiaobing Li (291454), Jing Wang (4865), Zhiqian Pang (544616)   +13 more
core   +1 more source

Additive‐Manufactured, Multifunctional Bioreactor Technology for Dynamic Culture of 3D Bioprinted Tissue Models

Advanced Materials Technologies, EarlyView.
We introduce an additively manufactured bioreactor with a perfusion flow system and integrated temperature and pH sensors for skeletal muscle tissue biofabrication. The bioreactor's performance was evaluated by assessing the viability, spreading of the myoblast cells in a printed scaffold, and contraction of the isolated murine musculi interossei ...
Lys Sprenger, Johann Schorzmann, Hsuan‐Heng Lu, Muhammad Osama, Julian Bauer, Michael Haug, Oliver Friedrich, Aldo R. Boccaccini, Frank Döpper, Sahar Salehi   +9 more
wiley   +1 more source

Knockdown of 4.1Ba causes a reduction in the number of synapses at CaPs.

, 2018
(A) Labeling of 4.1Ba-GFP puncta colocalized with the presynaptic marker Synapsin 1/2 (Syn) and the postsynaptic marker panMAGUK (PM) at CaPs. Arrows indicate sites of colocalization. (B) Representative images of Syn and PM in Ctl MO, 4.1Ba MO, and 4.1Ba
Javier Fierro Jr. (5819906), Philip Washbourne (2922252), Dylan R. Haynes (5819909)   +2 more
core   +1 more source

Piezo2 Mediates a Vicious Cycle of “Mechanical Homeostasis Imbalance—Inflammation” in Sensory Nerves and the Cartilage Endplate

Advanced Science, EarlyView.
In a lumbar spine instability model, dorsal root ganglion cells mediate the perception of relevant mechanical stresses through Piezo2 and subsequently release CGRP. CGRP activates the NF‐κB signaling pathway in cartilage endplate cells through the receptor RAMP1.
Hanpeng Xu, Wen Geng, Zhi Du, Yifan Du, Di Wu, Bide Tong, Huaizhen Liang, Xingyu Zhou, Zixuan Ou, Junyu Wei, Kun Wang, Yu Song, Wenbin Hua, Yan Xu, Wencan Ke, Bingjin Wang, Cao Yang   +16 more
wiley   +1 more source

Flavivirus RNA methylation

, 2014
The 5′ end of eukaryotic mRNA contains the type-1 (m7GpppNm) or type-2 (m7GpppNmNm) cap structure. Many viruses have evolved various mechanisms to develop their own capping enzymes (e.g. flavivirus and coronavirus) or to 'steal' caps from host mRNAs (e.g.
Roland , Zuest, Shi, Pei-Yong, Fink, Katja Fink, Lim, Siew Pheng, Dong, Hongping, Cheng-Feng , Qin   +5 more
core   +1 more source