Results 91 to 100 of about 29,994 (285)

Combining Chemical Synthesis and Enzymatic Methylation to Access Short RNAs with Various 5′ Caps

, 2019
International audienceEukaryotic RNAs are heavily processed, including co- and post-transcriptional formation of various 5' caps. In small nuclear RNAs (snRNAs) or small nucleolar RNAs (snoRNAs), the canonical 7m G cap is hypermethylated at the N2 ...
Théo Guez, Guez, Théo, Debart, Françoise, Andrea Rentmeister, Rentmeister, Andrea, Jean‐Jacques Vasseur, Muthmann, Nils, Nils Muthmann, Françoise Debart, Samie R. Jaffrey, Vasseur, Jean-Jacques, Jaffrey, Samie   +11 more
core   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Mitochondrial Genome of Fagus sylvatica L. as a Source for Taxonomic Marker Development in the Fagales

Plants, 2020
European beech, Fagus sylvatica L., is one of the most important and widespread deciduous tree species in Central Europe and is widely managed for its hard wood. The complete DNA sequence of the mitochondrial genome of Fagus sylvatica L.
Malte Mader, Hilke Schroeder, Thomas Schott, Katrin Schöning-Stierand, Ana Paula Leite Montalvão, Heike Liesebach, Mirko Liesebach, Barbara Fussi, Birgit Kersten   +8 more
doaj   +1 more source

c-Myc induced changes in higher order rDNA structure accompany growth factor stimulation of quiescent cells

, 2007
Human c-Myc is believed to be a high level coordinator of protein synthesis capacity and cell growth rate, capable of activating transcription by all three nuclear RNA Polymerases.
Rachel G. Berkson, Chiou-Nan Shiue, Anthony P. H. Wright   +2 more
core  

Microenvironmental Reprogramming by 3D Anisotropic Cardiac Extracellular Matrix Induces Nuclear Remodeling and Epigenetic Maturation of Chemically Induced Cardiomyocytes

Advanced Functional Materials, EarlyView.
A 3D anisotropic hydrogel derived from heart extracellular matrix guides cytoskeletal alignment and nuclear remodeling in reprogrammed cardiomyocyte‐like cells. This study reveals how matrix alignment modulates nuclear envelope dynamics and chromatin state, triggering transcriptional and functional maturation.
Seung Ju Seo, Mi Jeong Lee, Hyun Wook Kang, Seonhee Byeon, Soo‐Kyoung Choi, Nakwon Choi, Seung‐Woo Cho, Yoonhee Jin   +7 more
wiley   +1 more source

Solid Phase Synthesis and TAR RNA-Binding Activity of Nucleopeptides Containing Nucleobases Linked to the Side Chains via 1,4-Linked-1,2,3-triazole

Biomedicines
Nucleopeptides (NPs) represent synthetic polymers created by attaching nucleobases to the side chains of amino acid residues within peptides. These compounds amalgamate the characteristics of peptides and nucleic acids, showcasing a unique ability to ...
Piotr Mucha, Małgorzata Pieszko, Irena Bylińska, Wiesław Wiczk, Jarosław Ruczyński, Katarzyna Prochera, Piotr Rekowski   +6 more
doaj   +1 more source

Exploring an Alternative to mRNA Vaccine Cold Chain Storage: MRNA‐Lipid Nanoparticle Stability When Dried in a Polymer Matrix

Advanced Functional Materials, EarlyView.
The nanostructure, size, and function of mRNA‐loaded lipid nanoparticles are evaluated before drying, within polymer microneedles, and after rehydration. The results reveal the polymer and LNP loadings required to recover nanostructure and preserve the delivery performance in dry‐state formulations.
Brendan P. Dyett, Shahad K. Alsaiari, John L. Daristotle, Timothy A. Forster, Alicia Lau, Dhruv Varshney, Rania Afaneh, Andrew R. Hanna, Charles Sloane, David Mankus, Haitao Yu, Jiali Zhai, DongSoo Yun, Lisa H. Tostanoski, Abigail Lytton‐Jean, Dan H. Barouch, Charlotte E. Conn, Robert Langer, Ana Jaklenec, Calum J. Drummond   +19 more
wiley   +1 more source

Sulfated Glycoaminoglycans and Proteoglycan Syndecan-4 Are Involved in Membrane Fixation of LL-37 and Its Pro-Migratory Effect in Breast Cancer Cells

Biomolecules, 2019
Initially characterized by its antimicrobial activities, LL-37 has also been shown to significantly contribute to tumor development. On breast cancer cell lines, LL-37 increases intracellular calcium via the TRPV2 channel and their migration via the ...
Chahrazed Habes, Günther Weber, Caroline Goupille   +2 more
doaj   +1 more source

Mitochondrial RNA capping: highly efficient 5’-RNA capping with NAD ⁺ and NADH by yeast and human mitochondrial RNA polymerase

, 2018
Abstract Bacterial and eukaryotic nuclear RNA polymerases (RNAPs) cap RNA with the oxidized and reduced forms of the metabolic effector nicotinamide adenine dinucleotide, NAD + and NADH, using NAD + and NADH as non-canonical initiating nucleotides
Bird, Jeremy G., Basu, Urmimala, Kuster, David, Ramachandran, Aparna, Grudzien-Nogalska, Ewa, Kiledjian, Megerditch, Temiakov, Dmitry, Patel, Smita S., Ebright, Richard H., Nickels, Bryce E.   +9 more
openaire   +1 more source

Noncanonical metabolite RNA caps: Classification, quantification, (de)capping, and function

WIREs RNA, 2022
AbstractThe 5′ cap of eukaryotic mRNA is a hallmark for cellular functions from mRNA stability to translation. However, the discovery of novel 5′‐terminal RNA caps derived from cellular metabolites has challenged this long‐standing singularity in both eukaryotes and prokaryotes.
openaire   +2 more sources