Results 11 to 20 of about 708,746 (292)

Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design [PDF]

Biology, 2017
A bimolecular ribozyme consisting of a core ribozyme (ΔP5 RNA) and an activator module (P5abc RNA) has been used as a platform to design assembled RNA nanostructures.
Md. Motiar Rahman, Shigeyoshi Matsumura, Yoshiya Ikawa   +2 more
doaj   +4 more sources

Development of Triangle RNA Nanostructure for Enhancing RNAi-Mediated Control of Botrytis cinerea Through Spray-Induced Gene Silencing Without Extra Nanocarrier [PDF]

Biology
Botrytis cinerea, a necrotrophic fungal pathogen responsible for gray mold, poses a severe threat to over 1400 plant species, causing significant pre- and postharvest losses worldwide.
Ya Chen, Yiqing Liu, Yani Huang, Fangli Wu, Weibo Jin   +4 more
doaj   +2 more sources

Development of an RNA Nanostructure for Effective Botrytis cinerea Control through Spray-Induced Gene Silencing without an Extra Nanocarrier [PDF]

Journal of Fungi
Spray-induced gene silencing represents an eco-friendly approach for crop protection through the use of double-stranded RNA (dsRNA) to activate the RNA interference (RNAi) pathway, thereby silencing crucial genes in pathogens.
Fangli Wu, Ling Yan, Xiayang Zhao, Chongrun Lv, Weibo Jin   +4 more
doaj   +2 more sources

In vivo production of RNA nanostructures via programmed folding of single-stranded RNAs [PDF]

Nature Communications, 2018
RNA nanostructures have been demonstrated in a range of biological applications, but their assembly and delivery to cells is difficult. Here the authors demonstrate the in vivo assembly of a RNA nanostructure from a single transcript inside the cellular ...
Mo Li, Mengxi Zheng, Siyu Wu, Cheng Tian, Di Liu, Yossi Weizmann, Wen Jiang, Guansong Wang, Chengde Mao   +8 more
doaj   +3 more sources

Tetrahedral DNA dendritic nanostructure-enhanced FISH for high-speed, sensitive spatial transcriptomics [PDF]

Nature Communications
Understanding where genes are active within tissues is essential to explain how cells build and maintain organs, yet many spatial RNA assays are slow and weak, limiting short-transcript detection and masking cellular diversity.
Yi-Fan Wang, Hua-Jie Chen, Zhong-Da He, Zhi-Gang Wang, Dai-Wen Pang, Shu-Lin Liu   +5 more
doaj   +2 more sources

Designer RNA nanostructures co-transcribed and self-assembled inside human cell nuclei [PDF]

Nature Communications
The use of nucleic acid-based nanostructures as synthetic biological tools to interface with and regulate cell processes remains challenging. A major obstacle lies in nuclear delivery and retention within live eukaryotic cells.
Xu Chang, Maciej Jeziorek, Qi Yang, Edward M. Bonder, Hao Yan, Jean-Pierre Etchegaray, Fei Zhang   +6 more
doaj   +2 more sources

Flexible Hydrophobic Paper-Based Microfluidic Field-Effect Biosensor Amplified by RNA-Cleaving DNAzyme-Based DNA Nanostructure for Mg²⁺ Detection [PDF]

Biosensors
Magnesium ions (Mg2+) play an important role in animal health, with their concentration in the bloodstream serving as a key indicator for hypomagnesemia diagnosis.
Hui Wang, Yue He, Zhixue Yu, Ruipeng Chen, Zemeng Feng, Dongfei Chen, Waleid Mohamed El-Sayed Shakweer, Fan Zhang, Xuemei Nan, Mukaddas Mijit, Benhai Xiong, Liang Yang, Xiangfang Tang   +12 more
doaj   +2 more sources

Use of RNA structure flexibility data in nanostructure modeling [PDF]

Methods, 2011
In the emerging field of RNA-based nanotechnology there is a need for automation of the structure design process. Our goal is to develop computer methods for aiding in this process. Towards that end, we created the RNA junction database, which is a repository of RNA junctions, i.e.
Luc Jaeger, , Eckart Bindewald
exaly   +3 more sources

Programmable RNA Nanostructures Enable Nanopore Detection of Cotranscriptionally Introduced RNA Modifications. [PDF]

Nano Lett
Tracking RNA synthesis and metabolic histories requires co-transcriptional incorporation of modified nucleotides. However, identifying the incorporation of modified nucleotides in nascent RNA remains challenging, particularly for short RNAs. Here, we developed a method utilizing solid-state nanopores and DNA:RNA nanostructures to detect modified ...
Mohora I, Patiño Guillén G, Neis K, Valero J, Keyser UF, Bošković F.   +5 more
europepmc   +3 more sources

Amplification-free CRISPR/Cas13a-based electrochemical biosensor for the detection of circulating tumor RNA using RNA-3Ag⁺ probes on Au porous-lattice nanoelectrode [PDF]

Journal of Biological Engineering
Circulating tumor RNA (ctRNA) is a sensitive biomarker for early cancer diagnosis, offering real-time gene expression profiles and tumor-specific signatures missed by circulating tumor DNA.
Yu-Rim Lee, Myeong-Jun Lee, Sang-Nam Lee, Jeong-Woo Choi   +3 more
doaj   +2 more sources