, 2021 Robust methods are critical for testing the in vivo regulatory mechanism of RNA binding proteins. Here we report improvement of a protein–mRNA tethering assay to probe the function of an RNA binding protein in its natural context within the C.
Kimble, Judith +3 more
core +1 more source
Detection of genome-scale ordered RNA structure (GORS) in genomes of positive-stranded RNA viruses:Implications for virus evolution and host persistence [PDF]
, 2004 Discrete RNA secondary and higher-order structures, typically local in extent, play a fundamental role in RNA virus replication. Using new bioinformatics analysis methods, we have identified genome-scale ordered RNA structure (GORS) in many genera and ...
Evans, David J +5 more
core +1 more source
Domain orientation in the RNA helicase YxiN and the role of conformational changes for RNA unwinding [PDF]
, 2010 The RNA helicase YxiN from Bacillus subtilis is a member of the family of DEAD box proteins. YxiN is able to unwind RNA double strands in an ATP-dependent manner. The ability to catalyse RNA rearrangement is in vivo presumably necessary for the bacterial
Karow, Anne R.
core +1 more source
Ionizable lipid-assisted efficient hepatic delivery of gene editing elements for oncotherapy
Bioactive Materials, 2022 CRISPR/Cas9-based gene editing has emerged as a powerful biotechnological tool, that relies on Cas9 protein and single guided RNA (sgRNA) to edit target DNA.
Chunhui Li +11 more
doaj +1 more source
Nanostructured RNA for RNA Intereference
YAKUGAKU ZASSHI, 2013 RNA interference (RNAi) is a potent and highly specific gene-silencing phenomenon which is initiated or triggered by double-stranded RNAs (dsRNAs). Shortly after the development of RNAi, small interfering RNAs (siRNAs) that are 21 nucleotides in length with a 3' nucleotide overhang were shown to be very effective in mammalian cells.
openaire +3 more sources
Co-Delivery of mRNA and pDNA Using Thermally Stabilized Coacervate-Based Core-Shell Nanosystems
Pharmaceutics, 2021 Co-delivery of different species of protein-encoding polynucleotides, e.g., messenger RNA (mRNA) and plasmid DNA (pDNA), using the same nanocarrier is an interesting topic that remains scarcely researched in the field of nucleic acid delivery.
Sarah S. Nasr +5 more
doaj +1 more source
Trim25 is an RNA-specific activator of Lin28a/TuT4-mediated uridylation [PDF]
, 2014 RNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated
Nowak, Jakub S. +14 more
core +1 more source
The mutual interaction of glycolytic enzymes and RNA in post-transcriptional regulation
, 2022 Wegener M, Dietz K-J. The mutual interaction of glycolytic enzymes and RNA in post-transcriptional regulation. RNA . 2022.About three decades ago, researchers suggested that metabolic enzymes participate in cellular processes that are unrelated to their ...
Dietz, Karl-Josef +3 more
core +1 more source
Hybrid DNA/RNA nanostructures with 2′-5′ linkages [PDF]
Nanoscale, 2020 We report here the first instance of nucleic acid nanostructures that contain 2′-5′ linkages and characterize structures of different complexities: a simple duplex to a 4-arm junction, a double crossover (DX) motif and a tensegrity triangle motif.
Arun Richard Chandrasekaran +6 more
openaire +2 more sources
Adjuvanted RNA Origami—A Tunable Peptide Assembly Platform for Constructing Cancer Nanovaccines
VaccinesBackground/Objectives: Cancer peptide vaccines represent a promising strategy to develop targeted and personalized treatments for cancer patients.
Theresa Yip +4 more
doaj +1 more source