Results 1 to 10 of about 5,387 (170)
Molecular mechanisms of template-independent RNA polymerization by tRNA nucleotidyltransferases [PDF]
Frontiers in Genetics, 2014 The universal 3'-terminal CCA sequence of tRNA is built and/or synthesized by the CCA-adding enzyme, CTP:(ATP) tRNA nucleotidyltransferase. This RNA polymerase has no nucleic acid template, but faithfully synthesizes the defined CCA sequence on the 3 ...
Kozo eTomita, Seisueke eYamashita
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A phylogeny of bacterial RNA nucleotidyltransferases: Bacillus halodurans contains two tRNA nucleotidyltransferases. [PDF]
J Bacteriol, 2005 ABSTRACT We have analyzed the distribution of RNA nucleotidyltransferases from the family that includes poly(A) polymerases (PAP) and tRNA nucleotidyltransferases (TNT) in 43 bacterial species. Genes of several bacterial species encode only one member of the nucleotidyltransferase superfamily (NTSF), and if that protein functions
Bralley P, Chang SA, Jones GH.
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RNA minihelices as model substrates for ATP/CTP:tRNA nucleotidyltransferase [PDF]
Biochemical Journal, 1997 Twenty-one RNA minihelices, resembling the coaxially stacked acceptor- /T-stems and T-loop found along the top of a tRNA's three-dimensional structure, were synthesized and used as substrates for ATP/CTP:tRNA nucleotidyltransferases from Escherichia coli and Saccharomyces cerevisiae.
Zengji LI, Yue Sun, L. David THURLOW
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Nontemplated Terminal Nucleotidyltransferase Activity of Double-Stranded RNA Bacteriophage φ6 RNA-Dependent RNA Polymerase [PDF]
Journal of Virology, 2008 ABSTRACT The replication and transcription of double-stranded RNA (dsRNA) viruses occur within a polymerase complex particle in which the viral genome is enclosed throughout the entire life cycle of the virus. A single protein subunit in the polymerase complex is responsible for the template-dependent RNA polymerization activity. The isolated
Minna M. Poranen +2 more
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Reproducible and efficient new method of RNA 3’-end labelling by CutA nucleotidyltransferase-mediated CC-tailing [PDF]
RNA Biology, 2021 Despite the development of non-radioactive DNA/RNA labelling methods, radiolabelled nucleic acids are commonly used in studies focused on the determination of RNA fate. Nucleic acid fragments with radioactive nucleotide analoguesincorporated into the body or at the 5ʹ or 3ʹ terminus of the molecule can serve as probes in hybridization-based analyses of
Rafał Tomecki +4 more
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Transfer RNA nucleotidyltransferase repairs all transfer RNAs randomly
Journal of Molecular Biology, 1977 Total Escherichia coli transfer RNA was converted to tRNA-C-C by periodate oxidation and partially repaired with either E. coli or rabbit liver tRNA nucleotidyltransferase. Aminoacylation of the partially repaired molecules indicated that individual tRNA species were each repaired to the same extent as the total population of molecules.
Murray P. Deutscher, Jeffrey A. Evans
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Chemical Physics Letters, 2021 The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA-dependent RNA polymerase (RdRp) is a promising target for antiviral drugs. In this study, a chemical library (n = 300) was screened against the nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain.
Eleni Pitsillou +4 more
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Functions and mechanisms of
WIREs RNA, 2020 AbstractTermini often determine the fate of RNA molecules. In recent years, 3′ ends of almost all classes of RNA species have been shown to acquire nontemplated nucleotides that are added by terminal nucleotidyltransferases (TENTs). The best‐described role of 3′ tailing is the bulk polyadenylation of messenger RNAs in the cell nucleus that is catalyzed
Vladyslava Liudkovska +1 more
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A Member of the Polymerase β Nucleotidyltransferase Superfamily Is Required for RNA Interference in C. elegans [PDF]
Current Biology, 2005 RNA interference (RNAi) is an ancient, highly conserved mechanism in which small RNA molecules (siRNAs) guide the sequence-specific silencing of gene expression . Several silencing machinery protein components have been identified, including helicases, RNase-related proteins, double- and single-stranded RNA binding proteins, and RNA-dependent RNA ...
Chun-Chieh G. Chen +5 more
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Characterization of a Nodavirus Replicase Revealed a de Novo Initiation Mechanism of RNA Synthesis and Terminal Nucleotidyltransferase Activity [PDF]
Journal of Biological Chemistry, 2013 Nodaviruses are a family of positive-stranded RNA viruses with a bipartite genome of RNAs. In nodaviruses, genomic RNA1 encodes protein A, which is recognized as an RNA-dependent RNA polymerase (RdRP) and functions as the sole viral replicase protein responsible for its RNA replication.
Zhaowei Wang +8 more
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