Results 131 to 140 of about 4,731 (160)

Phylogeny and Evolution of RNA 3′-Nucleotidyltransferases in Bacteria

Journal of Molecular Evolution, 2019
The tRNA nucleotidyltransferases and poly(A) polymerases belong to a superfamily of nucleotidyltransferases. The amino acid sequences of a number of bacterial tRNA nucleotidyltransferases and poly(A) polymerases have been used to construct a rooted, neighbor-joining phylogenetic tree.
George H Jones
exaly   +3 more sources

Transfer RNA nucleotidyltransferase

, 1990
Publisher Summary All tRNA molecules contain, at the 3′ terminus, the identical-CCA sequence that is required for their acceptor and transfer functions. In prokaryotic cells, no discrete pattern has emerged. In laboratory strains of Escherichia coli , all tRNA genes sequenced encode the -CCA sequence, and mutant strains devoid of tRNA ...
Deutscher, Murray P, Murray P. Deutscher   +1 more
openaire   +3 more sources

The polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases

Current Opinion in Structural Biology, 2004
ATP- and NAD(+)-dependent DNA ligases, ATP-dependent RNA ligases and GTP-dependent mRNA capping enzymes comprise a superfamily of proteins that catalyze nucleotidyl transfer to polynucleotide 5' ends via covalent enzyme-(lysyl-N)-NMP intermediates. The superfamily is defined by five peptide motifs that line the nucleotide-binding pocket and contribute ...
Stewart Shuman, Christopher D Lima
exaly   +3 more sources

A tale of non-canonical tails: gene regulation by post-transcriptional RNA tailing

Nature Reviews Molecular Cell Biology, 2020
© 2020, Springer Nature Limited. RNA tailing, or the addition of non-templated nucleotides to the 3′ end of RNA, is the most frequent and conserved type of RNA modification.
Sha Yu, V Narry Kim, Kim V Narry
exaly   +2 more sources

Subcellular specialization of multifaceted 3′end modifying nucleotidyltransferases

Current Opinion in Cell Biology, 2012
While canonical 3'end modifications of mRNAs or tRNAs are well established, recent technological advances in RNA analysis have given us a glimpse of how widespread other types of distinctive 3'end modifications appear to be.
Christian R Eckmann
exaly   +1 more source

Transfer RNA nucleotidyltransferase repairs all transfer RNAs randomly

Journal of Molecular Biology, 1977
Total Escherichia coli transfer RNA was converted to tRNA-C-C by periodate oxidation and partially repaired with either E. coli or rabbit liver tRNA nucleotidyltransferase. Aminoacylation of the partially repaired molecules indicated that individual tRNA species were each repaired to the same extent as the total population of molecules.
M P, Deutscher, J A, Evans
openaire   +2 more sources

Biochemical and structural bioinformatics studies of fungal CutA nucleotidyltransferases explain their unusual specificity toward CTP and increased tendency for cytidine incorporation at the 3′-terminal positions of synthesized tails

Rna, 2017
Noncanonical RNA nucleotidyltransferases (NTases), including poly(A), poly(U) polymerases (PAPs/PUPs), and C/U-adding enzymes, modify 3′-ends of different transcripts affecting their functionality and stability.
Kamil Kobylecki, Andrzej Dziembowski, Krzysztof Ginalski   +2 more
exaly   +2 more sources

Purification of transfer-RNA-nucleotidyltransferase from E. Coli B

Biochemical and Biophysical Research Communications, 1970
Abstract Transfer-RNA-nucleotidyltransferase has been purified 1400-fold from E. coli B by liquid polymer phase fractionation and chromatography on DEAE-cellulose, hydroxylapatite, and QAE-Sephadex. The final preparation contains no detectable RNase I, RNase II, polynucleotide phosphorylase, ATPase, CTPase, or any of the aminoacyl-tRNA ...
J P, Miller, G R, Philipps
openaire   +2 more sources

t-RNA-nucleotidyltransferase activity in Lupinus luteus seeds

Phytochemistry, 1975
Abstract t -RNA-nucleotidyltransferase activity was detected in Lupinus luteus seed.The enzyme was partly purified, and some of its properties are described.
Henryk Cudny, Maciej Pietrzak, Slawomir Bartkowiak   +2 more
openaire   +1 more source

Transfer RNA metabolism in Escherichia coli cells deficient in tRNA nucleotidyltransferase

Journal of Molecular Biology, 1977
We have investigated the role of tRNA nucleotidyltransferase in transfer RNA metabolism using mutants ( cca mutants) of Escherichia coli deficient in this enzyme. Extracts of the most defective strain contained cca mutation into a variety of genetic backgrounds. However, tRNA from stationary phase cells was somewhat more defective.
M P, Deutscher, J J, Lin, J A, Evans
openaire   +2 more sources