tRNA Nucleotidyltransferase‐Catalyzed Incorporation of CMP and AMP into RNA‐Bacteriophage Genome Fragments [PDF]
European Journal of Biochemistry, 1975 Fragments of bacteriophage RNAs R17, MS2 and Qβ obtained by incubation with commercial snake venom phosphodiesterase become substrates of the Escherichia coli tRNA nucleotidyltransferase. The transferase adds back CMP and AMP in conditions in which it remains highly specific of CCA‐deprived tRNAs.
Alain Prochiantz +3 more
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U2 Small Nuclear RNA Is a Substrate for the CCA-adding Enzyme (tRNA Nucleotidyltransferase) [PDF]
Journal of Biological Chemistry, 2002 The CCA-adding enzyme builds and repairs the 3' terminus of tRNA. Approximately 65% of mature human U2 small nuclear RNA (snRNA) ends in 3'-terminal CCA, as do all mature tRNAs; the other 35% ends in 3' CC or possibly 3' C. The 3'-terminal A of U2 snRNA cannot be encoded because the 3' end of the U2 snRNA coding region is CC/CC, where the slash ...
HyunDae D. Cho +3 more
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Translocation and Rotation of tRNA during Template-Independent RNA Polymerization by tRNA Nucleotidyltransferase [PDF]
Structure, 2014 The 3'-terminal CCA (CCA-3' at positions 74-76) of tRNA is synthesized by CCA-adding enzyme using CTP and ATP as substrates, without a nucleic acid template. In Aquifex aeolicus, CC-adding and A-adding enzymes collaboratively synthesize the CCA-3'. The mechanism of CCA-3' synthesis by these two enzymes remained obscure.
Seisuke Yamashita +2 more
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Turnip Yellow Mosaic Virus RNA as a Substrate of the Transfer RNA Nucleotidyltransferase II. Incorporation of Cytidine 5′-Monophosphate and Determination of a Short Nucleotide Sequence at the 3′ End of the RNA [PDF]
Journal of Virology, 1973 Turnip yellow mosaic virus (TYMV) RNA treated with snake venom phosphodiesterase accepts cytidine 5′-monophosphate and adenosine 5′-monophosphate (AMP) when it is incubated in the presence of cytidine 5′-triphosphate (CTP), adenosine 5′-triphosphate, and Escherichia coli transfer RNA nucleotidyltransferase ...
S. Litvak +2 more
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Journal of Biological Chemistry, 1975 The biosynthesis of the phage T4-coded proline and serine transfer RNA species proceeds through a precursor RNA containing both tRNA sequences. Neither tRNA sequence in the precursor RNA contains the 3'-terminal C-C-A common to all mature tRNAs. Seidman and McClain ((1975) Proc. Natl. Acad. Sci. U. S. A.
FJ Schmidt
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Expanding the Available RNA Labeling Toolbox With CutA Nucleotidyltransferase for Efficient Transcript Labeling with Purine and Pyrimidine Nucleotide Analogs [PDF]
ChemBioChemAbstractRNA labeling is an invaluable tool for investigation of the function and localization of nucleic acids. Labels are commonly incorporated into 3′ end of RNA and the primary enzyme used for this purpose is RNA poly(A) polymerase (PAP), which belongs to the class of terminal nucleotidyltransferases (NTases).
Rafał Tomecki +5 more
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Mutation in PNPT1 , which Encodes a Polyribonucleotide Nucleotidyltransferase, Impairs RNA Import into Mitochondria and Causes Respiratory-Chain Deficiency [PDF]
The American Journal of Human Genetics, 2012 Multiple-respiratory-chain deficiency represents an important cause of mitochondrial disorders. Hitherto, however, mutations in genes involved in mtDNA maintenance and translation machinery only account for a fraction of cases. Exome sequencing in two siblings, born to consanguineous parents, with severe encephalomyopathy, choreoathetotic movements ...
Vanessa Vedrenne +12 more
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TYMV RNA As a substrate of the tRNA nucleotidyltransferase [PDF]
FEBS Letters, 1970 It has been recently shown that in the presence of partly purified enzyme preparations from Escherichia coli and ATP. lhe RNA (M.W. = 2 × 106) of Turnip Yellow Mosaic Virus (TYMV) reacts with valine forming an ester bond analogous to that existing in aminoacyltRNA: lhe valine estcrifies the., -terminal adenosine ofTYMV RNA II. 21.
S. Litvak +2 more
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Regulation of RNA stability by terminal nucleotidyltransferases [PDF]
, 2019 The dysregulation of RNAs has global effects on all cellular pathways. The regulation of RNA metabolism is thus tightly controlled. Terminal RNA nucleotidyltransferases (TENTs) regulate RNA stability and activity through the addition of non-templated nucleotides to the 3′-end.
Christina Z. Chung
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tRNA‐nucleotidyltransferases: Highly unusual RNA polymerases with vital functions [PDF]
FEBS Letters, 2009 tRNA‐nucleotidyltransferases are fascinating and unusual RNA polymerases responsible for the synthesis of the nucleotide triplet CCA at the 3′‐terminus of tRNAs. As this CCA end represents an essential functional element for aminoacylation and translation, these polymerases (CCA‐adding enzymes) are of vital importance in all organisms.
Stefan Vörtler, Mario Mörl
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