Results 221 to 230 of about 186,287 (262)

Splicing of messenger RNA precursors

BioEssays, 1985
AbstractThe splicing of pre‐mRNAs in vitro is accomplished by formation of RNA intermediates in a lariat form. Lariat RNAs have been recently identified in vivo supporting the validity of the proposed pathway for processing pre‐mRNAs in vitro.We have recently reported20 a partial purification scheme for a pre‐mRNA splicing activity. Purification of the
Satish Patwardhan, Gustavo Kaltwasser, Peter R. Dimaria, Carlos J. Goldenberg   +3 more
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A precursor of globin messenger RNA

Journal of Molecular Biology, 1976
Abstract The size of pulse-labeled globin messenger RNA nucleotide sequences was investigated, to determine whether newly transcribed globin mRNA molecules are larger than steady-state globin mRNA. Molecular hybridization techniques were used to compare directly the sedimentation of steady-state (unlabeled) and pulse-labeled (radioactive) globin mRNA
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Enzymatic N-riboside scission in RNA and RNA precursors

Current Opinion in Chemical Biology, 1997
N-ribohydrolases and transferases act on nucleosides, nucleotides and oligonucleotides to effect base removal. Advances in mechanistic and structural analysis have established that enzymes of N-riboside scission act by combinations of leaving-group and ribosyl activation.
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A precursor for a small stable RNA (10Sa RNA) ofEscherichia coli

Molecular and General Genetics MGG, 1989
Strains carrying plasmids that code for 10Sa RNA synthesize a larger molecule when the RNA processing enzyme RNase E is inactivated. The T1 fingerprint of 10Sa RNA and the larger molecule is very similar, but the latter contains additional oligonucleotides. We show that the larger RNA is converted to the smaller, mature RNA.
M N, Subbarao, D, Apirion
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Lariat RNA's as Intermediates and Products in the Splicing of Messenger RNA precursors

Science, 1984
The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5′ end of the intervening sequence joined to a site near the 3′ splice site. This lariat structure, which has been characterized for an adenovirus 2 major late transcript, has a branch point, with 2′-5′ and 3′-5′ phosphodiester bonds emanating from a single ...
R A, Padgett, M M, Konarska, P J, Grabowski, S F, Hardy, P A, Sharp   +4 more
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An axial binding site in the Tetrahymena precursor RNA

Journal of Molecular Biology, 1991
Previous studies allow the construction of three distinct models of the binding of G and arginine within the active site of the Tetrahymena self-splicing preribosomal precursor RNA. These models (base triple, axial I and axial II) are now distinguished by measurements on the specificity of RNAs with nucleotide substitutions at positions spanning the ...
M, Yarus, M, Illangesekare, E, Christian
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RNA Required for Import of Precursor Proteins into Mitochondria

Science, 1984
A cytoplasmic RNA moiety is necessary for posttranslational uptake of nuclear-encoded mammalian proteins destined for the mitochondrial matrix. Post-translational addition of ribonuclease to a reticulocyte lysate-programmed cell-free translation mixture inhibited subsequent import of six different mitochondrial matrix enzyme precursors into rat liver ...
F A, Firgaira, J P, Hendrick, F, Kalousek, J P, Kraus, L E, Rosenberg   +4 more
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Transcription and Processing of Transfer RNA Precursors

1976
Publisher Summary This chapter discusses the transcription of tRNA genes as precursors and how these are converted to mature tRNA. One important step in tRNA biosynthesis, the modification of tRNA bases, is discussed only in relation to the precursors.
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Dimeric transfer RNA precursors in S. pombe

Cell, 1980
Sequence analysis of a Schizosaccharomyces pombe DNA fragment revealed two tRNA coding regions separated by a seven nucleotide spacer. the 5'-proximal tRNA gene encodes a tRNAUCGSer sequence, which is interrupted by a 16 nucleotide intron at the 3' side of the base adjacent to the anticodon.
J, Mao, O, Schmidt, D, Söll
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Asymmetric maturation of a dimeric transfer RNA precursor

Journal of Molecular Biology, 1980
Abstract Six of the eight transfer RNAs coded by bacteriophage T4 are synthesized via three dimeric precursor molecules. The sequences of two of these have been determined. Both of these precursors give rise to equimolar amounts of the cognate tRNA molecules in vivo .
C, Guthrie, C A, Scholla
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