Results 1 to 10 of about 616,337 (352)

Comparative evaluation of DNA and RNA probes for capture-based mitochondrial DNA next-generation sequencing [PDF]

BMC Biology
Background Probe-based liquid-phase hybridization capture is a powerful and commonly used approach for next-generation sequencing (NGS) of mitochondrial DNA (mtDNA).
Tianlei Sun, Shengjing Li, Yang Liu, Kaixiang Zhou, Jiamin Wang, Zhangwen Lei, Xu Guo, Jinliang Xing, Wenjie Guo   +8 more
doaj   +4 more sources

A PCR-Based Method for RNA Probes and Applications in Neuroscience [PDF]

Frontiers in Neuroscience, 2018
In situ hybridization (ISH) is a powerful technique that is used to detect the localization of specific nucleic acid sequences for understanding the organization, regulation, and function of genes.
Ruifang Hua, Ruifang Hua, Ruifang Hua, Shanshan Yu, Mugen Liu, Haohong Li, Haohong Li   +6 more
doaj   +2 more sources

Enzymatic synthesis of reactive RNA probes containing squaramate-linked cytidine or adenosine for bioconjugations and cross-linking with lysine-containing peptides and proteins [PDF]

Communications Chemistry
Protein-RNA interactions play important biological roles and hence reactive RNA probes for cross-linking with proteins are important tools in their identification and study.
Ivana Ivancová, Tania Sánchez Quirante, Marek Ondruš, Radek Pohl, Marta Vlková, Eva Žilecká, Evžen Bouřa, Michal Hocek   +7 more
doaj   +2 more sources

Imaging of endogenous RNA in live cells using sequence-activated fluorescent RNA probes. [PDF]

Nucleic Acids Res
Zheng H, Liu X, Liu L, Hu J, Chen X.
europepmc   +3 more sources

Development of a Highly Selective and Sensitive Fluorescent Probe for Imaging RNA Dynamics in Live Cells

Molecules, 2022
RNA imaging is of great importance for understanding its complex spatiotemporal dynamics and cellular functions. Considerable effort has been devoted to the development of small-molecule fluorescent probes for RNA imaging.
Lan Fang, Wen Shao, Shu-Tang Zeng, Gui-Xue Tang, Jia-Tong Yan, Shuo-Bin Chen, Zhi-Shu Huang, Jia-Heng Tan, Xiu-Cai Chen   +8 more
doaj   +1 more source

Identification of soybean trans-factors associated with plastid RNA editing sites [PDF]

Genetics and Molecular Biology, 2020
RNA editing is a posttranscriptional process that changes nucleotide sequences, among which cytosine-to-uracil by a deamination reaction can revert non-neutral codon mutations.
Nureyev F. Rodrigues, Fábio C. S. Nogueira, Gilberto B. Domont, Rogerio Margis   +3 more
doaj   +1 more source

Sets of RNA repeated tags and hybridization-sensitive fluorescent probes for distinct images of RNA in a living cell. [PDF]

PLoS ONE, 2010
BACKGROUND: Imaging the behavior of RNA in a living cell is a powerful means for understanding RNA functions and acquiring spatiotemporal information in a single cell.
Takeshi Kubota, Shuji Ikeda, Hiroyuki Yanagisawa, Mizue Yuki, Akimitsu Okamoto   +4 more
doaj   +1 more source

Quantification of virus-infected cells using RNA FISH-Flow

STAR Protocols, 2023
Summary: We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA.
Cody J. Warren, Arturo Barbachano-Guerrero, Devra Huey, Qing Yang, Emma R. Worden-Sapper, Jens H. Kuhn, Sara L. Sawyer   +6 more
doaj   +1 more source

RNA probes prepared in vitro [PDF]

Nature, 1984
P.F.R. Little
openalex   +2 more sources

Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide

Cells, 2021
Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations.
Lu Xiao, Joshua Labaer, Jia Guo
doaj   +1 more source