Results 1 to 10 of about 587,888 (232)
A PCR-Based Method for RNA Probes and Applications in Neuroscience [PDF]
Frontiers in Neuroscience, 2018 In situ hybridization (ISH) is a powerful technique that is used to detect the localization of specific nucleic acid sequences for understanding the organization, regulation, and function of genes.
Ruifang Hua +6 more
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Enzymatic synthesis of reactive RNA probes containing squaramate-linked cytidine or adenosine for bioconjugations and cross-linking with lysine-containing peptides and proteins [PDF]
Communications ChemistryProtein-RNA interactions play important biological roles and hence reactive RNA probes for cross-linking with proteins are important tools in their identification and study.
Ivana Ivancová +7 more
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Affinity Purification of RNA: Sequence-Specific Capture by Nonionic Morpholino Probes
BioTechniques, 1997 Nucleic acid isolation for amplification-based diagnostics requires techniques that do not co-purify inhibitors of DNA polymerases. Also, other requirements for an ideal sample preparation technology include ease of use, capability for automation, high ...
J.M. Wages +4 more
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Probing RNA Structure by Lead Cleavage [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 AbstractLead cleavage causes a transesterification reaction that breaks the 5′,3′‐phosphodiester backbone of RNA, leaving a 2′,3′‐cyclic phosphate and a 5′‐hydroxyl. Since the efficiency of the reaction at the 2′‐hydroxyl is related to steric and chemical constraints on particular 2′‐hydroxyls embedded in the RNA, this reaction can be used to examine ...
Tao Pan
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Probing RNA Structures with Hydroxyl Radicals [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 AbstractFe(II)‐EDTA can be used to conveniently generate hydroxyl radicals to promote cleavage of RNA at nucleotide resolution. Two procedures are described, involving the generation of free radicals from solvated molecular oxygen and from hydrogen peroxide added to the RNA solution.
Daniel W. Celander
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Chemical and Computer Probing of RNA Structure
, 1996 Ribonucleic acids (RNAs) are one of the most important types of biopolymers. RNAs play key roles in the storage and multiplication of genetic information. They are important in catalysis and RNA splicing and are the most important steps of translation.
Н. А. Колчанов +3 more
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Double-Color Fluorescence In Situ Hybridization with RNA Probes [PDF]
BioTechniques, 2003 X. Xi +4 more
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Molecules, 2022 RNA imaging is of great importance for understanding its complex spatiotemporal dynamics and cellular functions. Considerable effort has been devoted to the development of small-molecule fluorescent probes for RNA imaging.
Lan Fang +8 more
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Identification of soybean trans-factors associated with plastid RNA editing sites [PDF]
Genetics and Molecular Biology, 2020 RNA editing is a posttranscriptional process that changes nucleotide sequences, among which cytosine-to-uracil by a deamination reaction can revert non-neutral codon mutations.
Nureyev F. Rodrigues +3 more
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