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A PCR-Based Method for RNA Probes and Applications in Neuroscience [PDF]

Frontiers in Neuroscience, 2018
In situ hybridization (ISH) is a powerful technique that is used to detect the localization of specific nucleic acid sequences for understanding the organization, regulation, and function of genes.
Ruifang Hua, Ruifang Hua, Ruifang Hua, Shanshan Yu, Mugen Liu, Haohong Li, Haohong Li   +6 more
doaj   +2 more sources

Enzymatic synthesis of reactive RNA probes containing squaramate-linked cytidine or adenosine for bioconjugations and cross-linking with lysine-containing peptides and proteins [PDF]

Communications Chemistry
Protein-RNA interactions play important biological roles and hence reactive RNA probes for cross-linking with proteins are important tools in their identification and study.
Ivana Ivancová, Tania Sánchez Quirante, Marek Ondruš, Radek Pohl, Marta Vlková, Eva Žilecká, Evžen Bouřa, Michal Hocek   +7 more
doaj   +2 more sources

Comparative evaluation of DNA and RNA probes for capture-based mitochondrial DNA next-generation sequencing [PDF]

BMC Biology
Background Probe-based liquid-phase hybridization capture is a powerful and commonly used approach for next-generation sequencing (NGS) of mitochondrial DNA (mtDNA).
Tianlei Sun, Shengjing Li, Yang Liu, Kaixiang Zhou, Jiamin Wang, Zhangwen Lei, Xu Guo, Jinliang Xing, Wenjie Guo   +8 more
doaj   +2 more sources

HybriSeq: probe-based device-free single-cell RNA profiling [PDF]

Communications Biology
We have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and sequencing analysis of the probes. We have shown that HybriSeq can
Daniel Foyt, David Brown, Shuqin Zhou, Brittany Moser, Qin Zhu, Zev J. Gartner, Bo Huang   +6 more
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Post-transcriptional labeling by using Suzuki-Miyaura cross-coupling generates functional RNA probes. [PDF]

Nucleic Acids Res, 2018
Walunj MB, Tanpure AA, Srivatsan SG.
europepmc   +3 more sources

Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations. [PDF]

Sci Rep, 2017
Wang Q, Wang X, Tang PS, O'leary GM, Zhang M.   +4 more
europepmc   +3 more sources

Imaging of endogenous RNA in live cells using sequence-activated fluorescent RNA probes. [PDF]

Nucleic Acids Res
Zheng H, Liu X, Liu L, Hu J, Chen X.
europepmc   +3 more sources

Fluorogenic Covalent Probes for RNA. [PDF]

J Am Chem Soc
ABSTRACT Sequence-generalized fluorescent labels and stains for RNA can enable imaging, tracking and analysis of the biopolymer. However, current non-covalent RNA dyes are poorly selective for RNA over DNA, interact weakly with their target, and can show limited utility in cellular RNA staining due to poor selectivity and high background ...
Shin J, Kim MJ, Kool ET.
europepmc   +2 more sources

RNA probes prepared in vitro [PDF]

Nature, 1984
P.F.R. Little
openalex   +2 more sources

Development of a Highly Selective and Sensitive Fluorescent Probe for Imaging RNA Dynamics in Live Cells

Molecules, 2022
RNA imaging is of great importance for understanding its complex spatiotemporal dynamics and cellular functions. Considerable effort has been devoted to the development of small-molecule fluorescent probes for RNA imaging.
Lan Fang, Wen Shao, Shu-Tang Zeng, Gui-Xue Tang, Jia-Tong Yan, Shuo-Bin Chen, Zhi-Shu Huang, Jia-Heng Tan, Xiu-Cai Chen   +8 more
doaj   +1 more source