Results 11 to 20 of about 587,888 (232)
Uncovering the expression patterns of chimeric transcripts using surveys of affymetrix GeneChips. [PDF]
, 2010 BACKGROUND: A chimeric transcript is a single RNA sequence which results from the transcription of two adjacent genes. Recent studies estimate that at least 4% of tandem human gene pairs may form chimeric transcripts. Affymetrix GeneChip data are used to
da Silva Camargo, R +4 more
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Sets of RNA repeated tags and hybridization-sensitive fluorescent probes for distinct images of RNA in a living cell. [PDF]
PLoS ONE, 2010 BACKGROUND: Imaging the behavior of RNA in a living cell is a powerful means for understanding RNA functions and acquiring spatiotemporal information in a single cell.
Takeshi Kubota +4 more
doaj +1 more source
Probing the structure of RNAs in solution [PDF]
Nucleic Acids Research, 1987 During these last years, a powerful methodology has been developed to study the secondary and tertiary structure of RNA molecules either free or engaged in complex with proteins. This method allows to test the reactivity of every nucleotide towards chemical or enzymatic probes.
Chantal Ehresmann +5 more
openaire +3 more sources
Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide
Cells, 2021 Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations.
Lu Xiao, Joshua Labaer, Jia Guo
doaj +1 more source
Current Issues in Molecular Biology, 2021 Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity.
Waqar Ahmad +3 more
doaj +1 more source
Fe.bleomycin as a probe of RNA conformation [PDF]
Nucleic Acids Research, 1996 Two crystallographically defined tRNAs, yeast tRNAAsp and tRNAPhe, were used as substrates for oxidative cleavage by Fe.bleomycin to facilitate definition at high resolution of the structural elements in RNAs conducive to bleomycin binding and cleavage. Yeast tRNAAsp underwent cleavage at G45 and U66; yeast tRNAPhe was cleaved at four sites, namely G19,
Chris E. Holmes +4 more
openaire +3 more sources
Organism-specific rRNA capture system for application in next-generation sequencing.
PLoS ONE, 2013 RNA-sequencing is a powerful tool in studying RNomics. However, the highly abundance of ribosomal RNAs (rRNA) and transfer RNA (tRNA) have predominated in the sequencing reads, thereby hindering the study of lowly expressed genes.
Sai-Kam Li +6 more
doaj +1 more source
BioTechniques, 2015 Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (
Yusuf Murgha +6 more
doaj +1 more source
DNA hybridization to mismatched templates: a chip study [PDF]
, 2001 High-density oligonucleotide arrays are among the most rapidly expanding technologies in biology today. In the {\sl GeneChip} system, the reconstruction of the target concentration depends upon the differential signal generated from hybridizing the ...
A. Bonincontro +19 more
core +2 more sources
Probing the phenomics of noncoding RNA [PDF]
eLife, 2013 Genetic knockout experiments on mice confirm that some long noncoding RNA molecules have developmental functions.
openaire +4 more sources