Probing RNA Folding Pathways by RNA Fingerprinting [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 AbstractThis unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are thus difficult to study by solution‐based methods.
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Identification of artifactual microarray probe signals constantly present in multiple sample types
BioTechniques, 2012 The detection, identification, and quantitation of transcripts have evolved from simple Northern analysis, cDNA cloning, and sequencing to RT-PCR, microarrays, and now digital gene expression using ultra–high-throughput RNA sequencing (RNA-Seq).
Shihong Mao +3 more
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Human fibroblast interferon RNA transcripts of different sizes in poly(I).poly(C) induced cells [PDF]
, 1983 A
Content, Jean +3 more
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Molecular Therapy: Nucleic Acids, 2022 We successfully fabricated threose nucleic acid (TNA)-based probes for real-time monitoring of target miRNA levels in cells. Our TNA probe is comprised of a fluorophore-labeled TNA reporter strand by partially hybridizing to a quencher-labeled TNA that ...
Fei Wang +5 more
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Bio-Protocol, 2018 The protocol described here has been developed to detect RNA at the single cell level. Fluorescent probes hybridize to target RNAs and are detected by flow cytometry after multiple amplification steps. Different types of RNA can be detected such as mRNA,
Alice Coillard, Elodie Segura
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Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH. [PDF]
PLoS ONE, 2014 Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being ...
Reelina Basu +5 more
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Orchestrated transcription of biological processes in the marine picoeukaryote Ostreococcus exposed to light/dark cycles [PDF]
, 2010 Background: Picoeukaryotes represent an important, yet poorly characterized component of marine phytoplankton. The recent genome availability for two species of Ostreococcus and Micromonas has led to the emergence of picophytoplankton comparative ...
Bouget, François-Yves +7 more
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Error, reproducibility and sensitivity : a pipeline for data processing of Agilent oligonucleotide expression arrays [PDF]
, 2010 Background Expression microarrays are increasingly used to obtain large scale transcriptomic information on a wide range of biological samples. Nevertheless, there is still much debate on the best ways to process data, to design experiments and analyse ...
AR Dabney +26 more
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Interaction of yeast eIF4G with spliceosome components Implications in pre-mRNA processing events [PDF]
, 2009 International audienceAs evidenced from mammalian cells the eukaryotic translation initiation factor eIF4G has a putative role in nuclear RNA metabolism. Here we investigate whether this role is conserved in the yeast Saccharomyces cerevisiae.
Barrass, J. David +6 more
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Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression
BMC Biotechnology, 2004 Background RNA dot blot hybridization is a commonly used technique for gene expression assays. However, membrane based RNA dot/slot blot hybridization is time consuming, requires large amounts of RNA, and is less suited for parallel assays of more than ...
Yadetie Fekadu +4 more
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