Results 21 to 30 of about 587,888 (232)
Specific and non specific hybridization of oligonucleotide probes on microarrays [PDF]
, 2005 Gene expression analysis by means of microarrays is based on the sequence specific binding of mRNA to DNA oligonucleotide probes and its measurement using fluorescent labels. The binding of RNA fragments involving other sequences than the intended target
Binder, Hans, Preibisch, Stephan
core +3 more sources
Metabolic RNA labeling for probing RNA dynamics in bacteria [PDF]
Nucleic Acids Research, 2020 Abstract Metabolic labeling of RNAs with noncanonical nucleosides that are chemically active, followed by chemoselective conjugation with imaging probes or enrichment tags, has emerged as a powerful method for studying RNA transcription and degradation in eukaryotes.
Liying Meng +5 more
openaire +2 more sources
Fluorescent turn-on probes for wash-free mRNA imaging via covalent site-specific enzymatic labeling. [PDF]
, 2017 Investigating the many roles RNA plays in cellular regulation and function has increased demand for tools to explore RNA tracking and localization within cells.
Alexander, Seth C +2 more
core +2 more sources
Identification of artifactual microarray probe signals constantly present in multiple sample types
BioTechniques, 2012 The detection, identification, and quantitation of transcripts have evolved from simple Northern analysis, cDNA cloning, and sequencing to RT-PCR, microarrays, and now digital gene expression using ultra–high-throughput RNA sequencing (RNA-Seq).
Shihong Mao +3 more
doaj +1 more source
A Feature Selection Algorithm to Compute Gene Centric Methylation from Probe Level Methylation Data [PDF]
, 2016 DNA methylation is an important epigenetic event that effects gene expression during development and various diseases such as cancer. Understanding the mechanism of action of DNA methylation is important for downstream analysis.
Baur, Brittany, Bozdag, Serdar
core +18 more sources
Probing RNA Folding Pathways by RNA Fingerprinting [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 AbstractThis unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are thus difficult to study by solution‐based methods.
openaire +5 more sources
A Genetic Locus Regulates the Expression of Tissue-Specific mRNAs from Multiple Transcription Units [PDF]
, 1982 129 GIX- mice, unlike animals of the congeneic partner strain GIX+, do not express significant amounts of the retroviral antigens gp70 and p30. Evidence is presented indicating that the GIX phenotype is specified by a distinct regulatory gene acting on ...
Lerner, Richard A. +2 more
core +2 more sources
Bio-Protocol, 2018 The protocol described here has been developed to detect RNA at the single cell level. Fluorescent probes hybridize to target RNAs and are detected by flow cytometry after multiple amplification steps. Different types of RNA can be detected such as mRNA,
Alice Coillard, Elodie Segura
doaj +1 more source
Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH. [PDF]
PLoS ONE, 2014 Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being ...
Reelina Basu +5 more
doaj +1 more source
Insights into the development of chemical probes for RNA [PDF]
Nucleic Acids Research, 2018 Over the past decade, the RNA revolution has revealed thousands of non-coding RNAs that are essential for cellular regulation and are misregulated in disease. While the development of methods and tools to study these RNAs has been challenging, the power and promise of small molecule chemical probes is increasingly recognized.
Brittany S Morgan +2 more
openaire +3 more sources