Northern Blot with IR Fluorescent Probes: Strategies for Probe Preparation
Bio-Protocol, 2019 Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern).
Christopher Fields +4 more
doaj +1 more source
Detection of NASBA amplified bacterial tmRNA molecules on SLICSel designed microarray probes
BMC Biotechnology, 2011 Background We present a comprehensive technological solution for bacterial diagnostics using tmRNA as a marker molecule. A robust probe design algorithm for microbial detection microarray is implemented.
Toome Kadri +9 more
doaj +1 more source
Expression of PEG11 and PEG11AS transcripts in normal and callipyge sheep [PDF]
, 2004 BACKGROUND: The callipyge mutation is located within an imprinted gene cluster on ovine chromosome 18. The callipyge trait exhibits polar overdominant inheritance due to the fact that only heterozygotes inheriting a mutant paternal allele (paternal ...
Bidwell, Christopher A +5 more
core +2 more sources
SNP detection in mRNA in living cells using allele specific FRET probes. [PDF]
PLoS ONE, 2013 Live mRNA detection allows real time monitoring of specific transcripts and genetic alterations. The main challenge of live genetic detection is overcoming the high background generated by unbound probes and reaching high level of specificity with ...
Liya Dahan +4 more
doaj +1 more source
Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]
, 2016 Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen +2 more
core +1 more source
The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system [PDF]
, 2014 The regulated head-to-tail expression of Hox genes provides a coordinate system for the activation of specific programmes of cell differentiation according to axial level.
Akam +57 more
core +1 more source
Genomics Data, 2016 Malarial parasite P. falciparum, an apicomplexan protozoan has a 23.3 MB nuclear genome and encodes ~5600 transcripts. The genetic diversity of the parasite within and across geographical zones is a challenge to gene expression studies which are ...
Amit Kumar Subudhi +9 more
doaj +1 more source
The Long Noncoding RNA HEAL Regulates HIV-1 Replication through Epigenetic Regulation of the HIV-1 Promoter. [PDF]
, 2019 A major challenge in finding a cure for HIV-1/AIDS is the difficulty in identifying and eradicating persistent reservoirs of replication-competent provirus.
Chang, Kungyen +11 more
core +1 more source
Single-probe RNA FISH in Yeast
BIO-PROTOCOL, 2018 Quantitative profiling of mRNA expression is an important part of understanding the state of a cell. The technique of RNA Fluorescence In Situ Hybridization (FISH) involves targeting an RNA transcript with a set of 40 complementary fluorescently labeled DNA oligonucleotide probes.
Rasesh Y. Parikh +2 more
openaire +4 more sources
Deuterated nucleotides as chemical probes of RNA structure: a detailed protocol for the enzymatic synthesis of a complete set of nucleotides specifically deuterated at ribose carbons [PDF]
, 2015 We describe here a detailed protocol for the synthesis of ribonucleotides specifically deuterated at each ribose carbon atom. We synthesized 20 specifically deuterated ribonucleotides: ATP, CTP, GTP, and UTP, each of which contained one of five ...
Azad, Robert +2 more
core +3 more sources