Results 11 to 20 of about 1,457,994 (304)

Advances in application of single-cell RNA sequencing in cardiovascular research

Frontiers in Cardiovascular Medicine, 2022
Single-cell RNA sequencing (scRNA-seq) provides high-resolution information on transcriptomic changes at the single-cell level, which is of great significance for distinguishing cell subtypes, identifying stem cell differentiation processes, and ...
Yue Hu, Ying Zhang, Yutong Liu, Yan Gao, Tiantian San, Xiaoying Li, Xiaoying Li, Sensen Song, Binglong Yan, Zhuo Zhao, Zhuo Zhao   +10 more
doaj   +1 more source

Dual RNA-Seq of Mtb-Infected Macrophages In Vivo Reveals Ontologically Distinct Host-Pathogen Interactions

Cell Reports, 2020
Summary: Dissecting the in vivo host-pathogen interplay is crucial to understanding the molecular mechanisms governing control or progression of intracellular infections.
Davide Pisu, Lu Huang, Jennifer K. Grenier, David G. Russell   +3 more
doaj   +1 more source

RNA modifications detection by comparative Nanopore direct RNA sequencing

Nature Communications, 2021
Nanopore direct RNA Sequencing data contain information about the presence of RNA modifications, but their detection poses substantial challenges. Here the authors introduce Nanocompore, a new methodology for modification detection from Nanopore data.
Adrien Leger, Paulo P. Amaral, Luca Pandolfini, Charlotte Capitanchik, Federica Capraro, Valentina Miano, Valentina Migliori, Patrick Toolan-Kerr, Theodora Sideri, Anton J. Enright, Konstantinos Tzelepis, Folkert J. van Werven, Nicholas M. Luscombe, Isaia Barbieri, Jernej Ule, Tomas Fitzgerald, Ewan Birney, Tommaso Leonardi, Tony Kouzarides   +18 more
doaj   +1 more source

Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification [PDF]

, 2020
Understanding genome organization and gene regulation requires insight into RNA transcription, processing and modification. We adapted nanopore direct RNA sequencing to examine RNA from a wild-type accession of the model plant Arabidopsis thaliana and a ...
Barton, Geoffrey J., Gould, Peter D., Hall, Anthony J. W., Knop, Katarzyna, Mackinnon, Katarzyna, Parker, Matthew T., Schurch, Nicholas J., Sherwood, Anna V., Simpson, Gordon G.   +8 more
core   +3 more sources

MultiEditR: The first tool for the detection and quantification of RNA editing from Sanger sequencing demonstrates comparable fidelity to RNA-seq

Molecular Therapy: Nucleic Acids, 2021
We present MultiEditR (Multiple Edit Deconvolution by Inference of Traces in R), the first algorithm specifically designed to detect and quantify RNA editing from Sanger sequencing (z.umn.edu/multieditr).
Mitchell G. Kluesner, Rafail Nikolaos Tasakis, Taga Lerner, Annette Arnold, Sandra Wüst, Marco Binder, Beau R. Webber, Branden S. Moriarity, Riccardo Pecori   +8 more
doaj   +1 more source

A High-Throughput Method for Illumina RNA-Seq Library Preparation. [PDF]

, 2012
With the introduction of cost effective, rapid, and superior quality next generation sequencing techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments. However,
Daniel H Chitwood, Jie ePeng, Julin N Maloof, Lauren R Headland, Neelima R Sinha, Ravi eKumar, Seisuke eKimura, Seisuke eKimura, Yasunori eIchihashi   +8 more
core   +2 more sources

YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs. [PDF]

, 2017
Besides translation, transfer RNAs (tRNAs) play many non-canonical roles in various biological pathways and exhibit highly variable expression profiles.
Honda, Shozo, Kirino, Yohei, Loher, Phillipe, Rigoutsos, Isidore, Shigematsu, Megumi, Telonis, Aristeidis G.   +5 more
core   +2 more sources

Epidermal Growth Factor Receptor Mutation and Anaplastic Lymphoma Kinase Gene Fusion: Detection in Malignant Pleural Effusion by RNA or PNA Analysis. [PDF]

PLoS ONE, 2016
Analyzing EGFR mutations and detecting ALK gene fusion are indispensable when planning to treat pulmonary adenocarcinoma. Malignant pleural effusion (MPE) is a devastating complication of lung cancer and sometimes the only source for mutation analysis ...
Yi-Lin Chen, Chung-Ta Lee, Cheng-Chan Lu, Shu-Ching Yang, Wan-Li Chen, Yang-Cheng Lee, Chung-Hsien Yang, Shu-Ling Peng, Wu-Chou Su, Nan-Haw Chow, Chung-Liang Ho   +10 more
doaj   +1 more source

Advancing transcriptome platforms [PDF]

, 2011
During the last decade of years, remarkable technological innovations have emerged that allow the direct or indirect determination of the transcriptome at unprecedented scale and speed.
Shuobo Shi
core   +2 more sources

Detecting RNA modification using direct RNA sequencing: A systematic review

Computational and Structural Biotechnology Journal, 2022
Post-transcriptional RNA modifications are involved in a range of important cellular processes, including the regulation of gene expression and fine-tuning of the functions of RNA molecules.
Xichen Zhao, Yuxin Zhang, Daiyun Hang, Jia Meng, Zhen Wei   +4 more
doaj   +1 more source