Results 1 to 10 of about 540 (209)
Splice site m6A methylation prevents binding of U2AF35 to inhibit RNA splicing [PDF]
Cell, 2021 The N6-methyladenosine (m6A) RNA modification is used widely to alter the fate of mRNAs. Here we demonstrate that the C. elegans writer METT-10 (the ortholog of mouse METTL16) deposits an m6A mark on the 3' splice site (AG) of the S-adenosylmethionine (SAM) synthetase pre-mRNA, which inhibits its proper splicing and protein production. The mechanism is
Florian Steiner +2 more
exaly +5 more sources
Intronic sequences and 3' splice sites control Rous sarcoma virus RNA splicing [PDF]
Journal of Virology, 1992 cis-acting sequences of Rous sarcoma virus (RSV) RNA involved in control of the incomplete splicing that is part of the retroviral life cycle have been studied. The 5' and two alternative 3' splice sites, as well as negative regulator of splicing element in the intron, have been introduced into chimeric constructs, and their responsive roles in ...
K Beemon, M T Mcnally
exaly +3 more sources
Precursor RNA structural patterns at SF3B1 mutation sensitive cryptic 3’ splice sites
RNA BiologySF3B1 is a core component of the spliceosome involved in branch point recognition and 3’ splice site selection. The SF3B1 K700E mutation (lysine to glutamic acid) is common in myelodysplastic syndrome and other blood disorders.
Austin Herbert +5 more
doaj +3 more sources
Animal, Fungi, and Plant Genome Sequences Harbor Different Non-Canonical Splice Sites
Cells, 2020 Most protein-encoding genes in eukaryotes contain introns, which are interwoven with exons. Introns need to be removed from initial transcripts in order to generate the final messenger RNA (mRNA), which can be translated into an amino acid sequence ...
Katharina Frey, Boas Pucker
doaj +3 more sources
SOAPsplice: genome-wide ab initio detection of splice junctions from RNA-Seq data
Frontiers in Genetics, 2011 RNA-Seq, a method using next generation sequencing technologies to sequence the transcriptome, facilitates genome-wide analysis of splice junction sites.
Songbo eHuang +8 more
doaj +3 more sources
Virology, 1995 The human immunodeficiency virus type 1 (HIV-1) genome contains 20 exons that are alternatively spliced from 16 splice sites to generate more than 40 different mRNAs, including incompletely spliced and unspliced mRNAs. In contrast to avian retroviral RNA, which has a cis-acting element in gag that negatively regulates splicing (NRS), HIV-1 RNA did not ...
Karen Beemon +2 more
exaly +3 more sources
The Journal of Clinical Endocrinology & Metabolism, 2021 Abstract Context Congenital hypothyroidism (CH) is caused by mutations in the genes for thyroid hormone synthesis. In our previous investigation of CH patients, approximately 53% of patients had mutations in either coding exons or canonical splice sites of causative genes.
Najla Albader +7 more
openaire +2 more sources
In vivo nuclear RNA structurome reveals RNA-structure regulation of mRNA processing in plants
Genome Biology, 2021 Background mRNA processing is critical for gene expression. A challenge in regulating mRNA processing is how to recognize the actual mRNA processing sites, such as splice and polyadenylation sites, when the sequence content is insufficient for this ...
Zhenshan Liu +8 more
doaj +1 more source
Predicting the effect of variants on splicing using Convolutional Neural Networks [PDF]
PeerJ, 2020 Mutations that cause an error in the splicing of a messenger RNA (mRNA) can lead to diseases in humans. Various computational models have been developed to recognize the sequence pattern of the splice sites.
Thanyathorn Thanapattheerakul +2 more
doaj +2 more sources
Solution structure of the donor site of a trans-splicing RNA [PDF]
Structure, 1996 RNA splicing is both ubiquitous and essential for the maturation of precursor mRNA molecules in eukaryotes. The process of trans-splicing involves the transfer of a short spliced leader (SL) RNA sequence to a consensus acceptor site on a separate pre-mRNA transcript.
Greenbaum, Nancy L +3 more
openaire +2 more sources