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Definition of the Th/ To ribonucleoprotein by RNase P and RNase MRP
Molecular Biology Reports, 1993We show that the Th/To ribonucleoprotein is defined by (i) the co-immunoprecipitation of two RNAs, (ii) the co-immunoprecipitation of four major polypeptides and (iii) the quantitative immune recognition of both RNase P and RNase MRP. No serum was found that recognizes either one of these two enzymes exclusively.
W, Rossmanith, R, Karwan
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Eukaryote RNase P and RNase MRP
2009Ribonuclease P (RNase P) is an essential endonuclease that catalyzes the cleavage of the 5′ leader sequence from precursor tRNAs (pre-tRNAs). Most forms of RNase P are ribonucleoproteins and the bacterial enzyme possesses a single catalytic RNA and one small protein.
Scott C. Walker +2 more
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2003
A sensitive method for quantitation of mRNA in gene transfer studies is mRNA protection using end-labeled DNA probes. In addition, this technique also provides structural information about the transcript under study (1). In vitro labeled antisense RNAcan be used as an alternative to end-labeled DNA probes (2,3.
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A sensitive method for quantitation of mRNA in gene transfer studies is mRNA protection using end-labeled DNA probes. In addition, this technique also provides structural information about the transcript under study (1). In vitro labeled antisense RNAcan be used as an alternative to end-labeled DNA probes (2,3.
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Cold Spring Harbor Protocols, 2013
The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one [α-32P]NTP, usually [α-32P]UTP. The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters that have ...
Michael F, Carey +2 more
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The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one [α-32P]NTP, usually [α-32P]UTP. The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters that have ...
Michael F, Carey +2 more
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2023
RNase J is involved in RNA maturation as well as degradation of RNA to the level of mononucleotides. This enzyme plays a vital role in maintaining intracellular RNA levels and governs different steps of the cellular metabolism in bacteria. RNase J is the first ribonuclease that was shown to have both endonuclease and 5'-3' exonuclease activity. RNase J
Muralidharan, Vandanashree +2 more
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RNase J is involved in RNA maturation as well as degradation of RNA to the level of mononucleotides. This enzyme plays a vital role in maintaining intracellular RNA levels and governs different steps of the cellular metabolism in bacteria. RNase J is the first ribonuclease that was shown to have both endonuclease and 5'-3' exonuclease activity. RNase J
Muralidharan, Vandanashree +2 more
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The mystery of mitochondrial RNases
Biochemical Society Transactions, 2012The central dogma states that DNA is transcribed to generate RNA and that the mRNA components are then translated to generate proteins; a simple statement that completely belies the complexities of gene expression. Post-transcriptional regulation alone has many points of control, including changes in the stability, translatability or susceptibility to ...
Bruni F +3 more
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The multifunctional RNase XRN2
Biochemical Society Transactions, 2013Different classes of RNA function in various cellular processes, and their biogenesis and turnover involve diverse RNases for processing and degradation. XRN2 is a 5′→3′ exoribonuclease that is evolutionarily conserved in eukaryotes. It is predominantly localized in the nucleus and recognizes single-stranded RNA with a 5′-terminal monophosphate to ...
Miki TS, Großhans H
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2019
Unbound, single-stranded RNA can be digested by RNase (A or T1) to ribonucleotides, whereas double-stranded RNA is not digested by RNase. Based on this principle, the RNase Protection Assay (RPA) is used to validate chimeric RNAs. Importantly, this assay does not employ reverse transcription (RT), thus avoiding potential false-positive results which ...
Jianzhu, Zhao +3 more
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Unbound, single-stranded RNA can be digested by RNase (A or T1) to ribonucleotides, whereas double-stranded RNA is not digested by RNase. Based on this principle, the RNase Protection Assay (RPA) is used to validate chimeric RNAs. Importantly, this assay does not employ reverse transcription (RT), thus avoiding potential false-positive results which ...
Jianzhu, Zhao +3 more
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RNase MRP/RNase P: a structure-function relation conserved in evolution? [PDF]
FEBS Letters, 1993 RNase P and RNase MRP are related ribonucleoproteins. RNase MRP processes mitochondrial precursor- (primer) RNAs, whereas RNase P cleaves precursor-tRNAs to produce their mature 5'-ends.
Robert Karwan, Karwan, Robert
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Trends in Biochemical Sciences, 1988
Abstract Cells contain an unexpectedly large number of distinct ribonucleases (RNases). Through a combination of various strategies these enzymes carry out the many specific reactions of RNA metabolism, but avoid the wholesale degradation of cellular RNA.
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Abstract Cells contain an unexpectedly large number of distinct ribonucleases (RNases). Through a combination of various strategies these enzymes carry out the many specific reactions of RNA metabolism, but avoid the wholesale degradation of cellular RNA.
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