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Bacteria deliver a microtubule-binding protein into mammalian cells to promote colonization. [PDF]
Costello MS +20 more
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2019
Unbound, single-stranded RNA can be digested by RNase (A or T1) to ribonucleotides, whereas double-stranded RNA is not digested by RNase. Based on this principle, the RNase Protection Assay (RPA) is used to validate chimeric RNAs. Importantly, this assay does not employ reverse transcription (RT), thus avoiding potential false-positive results which ...
Jianzhu, Zhao +3 more
openaire +2 more sources
Unbound, single-stranded RNA can be digested by RNase (A or T1) to ribonucleotides, whereas double-stranded RNA is not digested by RNase. Based on this principle, the RNase Protection Assay (RPA) is used to validate chimeric RNAs. Importantly, this assay does not employ reverse transcription (RT), thus avoiding potential false-positive results which ...
Jianzhu, Zhao +3 more
openaire +2 more sources
Eukaryote RNase P and RNase MRP
2009Ribonuclease P (RNase P) is an essential endonuclease that catalyzes the cleavage of the 5′ leader sequence from precursor tRNAs (pre-tRNAs). Most forms of RNase P are ribonucleoproteins and the bacterial enzyme possesses a single catalytic RNA and one small protein.
Scott C. Walker +2 more
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Is RNase V a manifestation of RNase II?
Biochemical and Biophysical Research Communications, 1971Abstract The relationship between RNase II and RNase V activities in Escherichia coli was investigated. The experiments suggest the possibility that the activity named RNase V was due to residual RNase II activity or a related enzyme on ribosomes.
A L, Bothwell, D, Apirion
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Nature Plants, 2020
Plants have developed a variety of molecular ways to express self-incompatibility (SI) that promote outcrossing by preventing self-fertilization. A new study reveals that Citrus uses the S-RNase as the key molecule for expressing SI, giving us further evidence that the S-RNase system is widespread and evolved in an early branch of angiosperms.
Sota Fujii, Seiji Takayama
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Plants have developed a variety of molecular ways to express self-incompatibility (SI) that promote outcrossing by preventing self-fertilization. A new study reveals that Citrus uses the S-RNase as the key molecule for expressing SI, giving us further evidence that the S-RNase system is widespread and evolved in an early branch of angiosperms.
Sota Fujii, Seiji Takayama
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2007
The RNase protection assay is a standard approach to determine mRNA levels of a gene of interest in different tissues, developmental stages, or times of the day. Splicing or promoter variants can be studied with specific probes. It is widely used in chronobiology to study the temporal profile of expression of circadian genes and the effects of genetic ...
openaire +3 more sources
The RNase protection assay is a standard approach to determine mRNA levels of a gene of interest in different tissues, developmental stages, or times of the day. Splicing or promoter variants can be studied with specific probes. It is widely used in chronobiology to study the temporal profile of expression of circadian genes and the effects of genetic ...
openaire +3 more sources
Cold Spring Harbor Protocols, 2013
The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one [α-32P]NTP, usually [α-32P]UTP. The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters that have ...
Michael F, Carey +2 more
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The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one [α-32P]NTP, usually [α-32P]UTP. The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters that have ...
Michael F, Carey +2 more
openaire +2 more sources
Nature, 2008
The universality of ribonuclease P (RNase P), the ribonucleoprotein essential for transfer RNA (tRNA) 5' maturation, is challenged in the archaeon Nanoarchaeum equitans. Neither extensive computational analysis of the genome nor biochemical tests in cell extracts revealed the existence of this enzyme.
Lennart, Randau +2 more
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The universality of ribonuclease P (RNase P), the ribonucleoprotein essential for transfer RNA (tRNA) 5' maturation, is challenged in the archaeon Nanoarchaeum equitans. Neither extensive computational analysis of the genome nor biochemical tests in cell extracts revealed the existence of this enzyme.
Lennart, Randau +2 more
openaire +2 more sources

