Results 61 to 70 of about 14,330,246 (353)
High-speed DNA-based rolling motors powered by RNase H
Nature Nanotechnology, 2015 DNA-based machines that walk by converting chemical energy into controlled motion could be of use in applications such as next generation sensors, drug delivery platforms, and biological computing.
Kevin Yehl +7 more
semanticscholar +1 more source
Recombining the structures of HIV integrase, RuvC and RNase H [PDF]
Structure, 1995 The recently reported crystal structures of two recombination enzymes, the catalytic domain of HIV integrase and Escherichia coli RuvC, an endonuclease, are surprisingly similar to that of ribonuclease H suggesting the possibility that they have a common enzymatic mechanism.
Thomas A. Steitz, Wei Yang
openaire +3 more sources
BioTechniques, 2002 None of the available antiretroviral drugs that are currently used in the clinic to treat infection with HIV-1 is directed against the RNase H active site of the reverse transcriptase.
N. McLellan +4 more
doaj +1 more source
Detection of an RNase H activity associated with hepadnaviruses
Journal of Virology, 1995 Replication of the hepadnavirus DNA genome is accomplished via reverse transcription of an intermediate, pregenomic RNA molecule. This process is likely to be carried out by a virally encoded, multifunctional polymerase which possesses DNA- and RNA-dependent DNA polymerase and RNase H activities.
John E. Newbold, S M Oberhaus
openaire +4 more sources
Mechanism of polypurine tract primer generation by HIV-1 reverse transcriptase [PDF]
, 2018 HIV-1 reverse transcriptase (HIV-1 RT) possesses both DNA polymerase activity and RNase H activity that act in concert to convert single-stranded RNA of the viral genome to double-stranded DNA that is then integrated into the DNA of the infected cell ...
Figiel, Małgorzata +8 more
core +1 more source
The RNase J-Based RNA Degradosome Is Compartmentalized in the Gastric Pathogen
mBio, 2020 Posttranscriptional regulation is a major level of gene expression control in any cell. In bacteria, multiprotein machines called RNA degradosomes are central for RNA processing and degradation, and some were reported to be compartmentalized inside these
Alejandro Tejada-Arranz +5 more
doaj +1 more source
Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples [PDF]
, 2014 RNA-Seq is an effective method to study the transcriptome, but can be difficult to apply to scarce or degraded RNA from fixed clinical samples, rare cell populations, or cadavers.
Adiconis, Xian +13 more
core +1 more source
Scientific Reports, 2018 Taking into account the important role of miRNA in carcinogenesis, oncogenic miRNAs are attractive molecules for gene-targeted therapy. Here, we developed a novel series of peptide-oligonucleotide conjugates exhibiting ribonuclease activity targeted to ...
O. Patutina +6 more
semanticscholar +1 more source
Evolutionary interplay between viruses and R‐loops
FEBS Letters, EarlyView.Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi +4 more
wiley +1 more source
FEBS Letters, EarlyView.This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao +7 more
wiley +1 more source