Results 71 to 80 of about 437,487 (317)

RNase H and replication of ColE1 DNA in Escherichia coli [PDF]

Journal of Bacteriology, 1986
Amber mutations within the rnh (RNase H) gene of Escherichia coli K-12 were isolated by selecting for bacteria capable of replicating in a sup+ background replication-defective cer-6 mutant of the ColE1 replicon. The cer-6 mutation is an alteration of one base pair located 160 nucleotides upstream of the unique replication origin of this plasmid ...
S Naito, H Uchida
openaire   +3 more sources

The anticancer effect of the HDAC inhibitor belinostat is enhanced by inhibitors of Bcl‐xL or Mcl‐1 in ovarian cancer

Molecular Oncology, EarlyView.
The pan‐HDAC inhibitor belinostat increases the expression of the pro‐apoptotic proteins Bim, Puma, and Noxa and induces apoptosis in ovarian cancer cell lines and patient‐derived tumor organoids when used at high concentrations. Moreover, inhibiting the anti‐apoptotic proteins Bcl‐xL or Mcl‐1 sensitizes these preclinical models to the cytotoxic effect
Cécilia Thomine, Sterenn Guillemot, Louis‐Bastien Weiswald, Romane Florent, Edwige Abeilard, Florence Giffard, Emilie Brotin, Mélanie Briand, Enora Dolivet, Laurent Poulain, Marie Villedieu   +10 more
wiley   +1 more source

Characterization of Nucleoside Reverse Transcriptase Inhibitor-Associated Mutations in the RNase H Region of HIV-1 Subtype C Infected Individuals

Viruses, 2017
The South African national treatment programme includes nucleoside reverse transcriptase inhibitors (NRTIs) in both first and second line highly active antiretroviral therapy regimens.
Sinaye Ngcapu, Kristof Theys, Pieter Libin, Vincent C. Marconi, Henry Sunpath, Thumbi Ndung’u, Michelle L. Gordon   +6 more
doaj   +1 more source

In Vitro Anti-HIV-1 Reverse Transcriptase and Integrase Properties of Punica granatum L. Leaves, Bark, and Peel Extracts and Their Main Compounds

Plants, 2021
In a search for natural compounds with anti-HIV-1 activity, we studied the effect of the ethanolic extract obtained from leaves, bark, and peels of Punica granatum L.
Cinzia Sanna, Arianna Marengo, Stefano Acquadro, Alessia Caredda, Roberta Lai, Angela Corona, Enzo Tramontano, Patrizia Rubiolo, Francesca Esposito   +8 more
doaj   +1 more source

Tumor clusters with divergent inflammation and human retroelement expression determine the clinical outcome of patients with serous ovarian cancer

Molecular Oncology, EarlyView.
Analysis of treatment‐naïve high‐grade serous ovarian carcinoma (HGSOC) and control tissues for ERVs, LINE‐1 (L1), inflammation, and immune checkpoints identified five clusters with diverse patient recurrence‐free survivals. An inflammation score was calculated and correlated with retroelement expression, where one novel cluster (Triple‐I) with high ...
Laura Glossner, Markus Eckstein, Christoph Mark, Matthias W. Beckmann, Arndt Hartmann, Pamela L. Strissel, Reiner Strick   +6 more
wiley   +1 more source

One-Pot Production of RNA in High Yield and Purity Through Cleaving Tandem Transcripts

Molecules, 2020
There is an increasing demand for efficient and robust production of short RNA molecules in both pharmaceutics and research. A standard method is in vitro transcription by T7 RNA polymerase.
Hannes Feyrer, Raluca Munteanu, Lorenzo Baronti, Katja Petzold   +3 more
doaj   +1 more source

Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. [PDF]

PLoS ONE, 2014
Antisense oligonucleotides (ASOs) are most commonly designed to reduce targeted RNA via RNase H1-dependent degradation. In this paper we demonstrate that cellular proteins can compete for sites targeted by RNase H1-dependent ASOs.
Timothy A Vickers, Stanley T Crooke
doaj   +1 more source

Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples [PDF]

, 2014
RNA-Seq is an effective method to study the transcriptome, but can be difficult to apply to scarce or degraded RNA from fixed clinical samples, rare cell populations, or cadavers.
Adiconis, Xian, Berlin, Aaron M., Borges-Rivera, Diego, Busby, Michele A., DeLuca, David S., Fennell, Timothy, Gnirke, Andreas, Levin, Joshua Z., Pochet, Nathalie, Regev, Aviv, Satija, Rahul, Sivachenko, Andrey, Thompson, Dawn Anne, Wysoker, Alec   +13 more
core   +1 more source

A DIA‐MS‐based proteomics approach to find potential serum prognostic biomarkers in glioblastoma patients

Molecular Oncology, EarlyView.
A DIA‐MS‐based proteomics analysis of serum samples from GB patients and healthy controls showed that high levels of IL1R2 and low levels of CRTAC1 and HRG in serum are associated with poor survival outcomes for GB patients. These circulating proteins could serve as biomarkers for the prediction of outcome in patients with GB.
Anne Clavreul, François Guillonneau, Odile Blanchet, Hamza Lasla, Audrey Rousseau, Catherine Guette, Alice Boissard, Cécile Henry, Morgan Dhondt, Pascal Jézéquel, Philippe Menei, Jean‐Michel Lemée   +11 more
wiley   +1 more source

Mechanism of polypurine tract primer generation by HIV-1 reverse transcriptase [PDF]

, 2018
HIV-1 reverse transcriptase (HIV-1 RT) possesses both DNA polymerase activity and RNase H activity that act in concert to convert single-stranded RNA of the viral genome to double-stranded DNA that is then integrated into the DNA of the infected cell ...
Figiel, Małgorzata, Gołąb, Agnieszka, Ha, Taekjip, Krepl, Miroslav, Nowotny, Marcin, Park, Sangwoo, Poznański, Jarosław, Skowronek, Krzysztof, Šponer, Jiři   +8 more
core   +1 more source