Proteins Rpr2 and Pop3 increase the activity and thermal stability of yeast RNase P [PDF]
RNA Biology, 2023 RNA-based enzyme RNase P is a ribonucleoprotein complex responsible primarily for 5’-maturation of tRNAs. S. cerevisiae RNase P comprises a catalytic RNA component and nine proteins. The assembly and maturation of S.
Anna Perederina +2 more
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An RNA ligase partner for the prokaryotic protein-only RNase P: insights into the functional diversity of RNase P from genome mining [PDF]
mBioRNase P can use either an RNA- or a protein-based active site to catalyze 5′-maturation of transfer RNAs (tRNAs). This distinctive attribute in the biocatalytic repertoire raises questions about the underlying evolutionary driving forces, especially if ...
Rekha Seshadri, Venkat Gopalan
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Structure and mechanistic features of the prokaryotic minimal RNase P [PDF]
eLife, 2021 Endonucleolytic removal of 5’-leader sequences from tRNA precursor transcripts (pre-tRNAs) by ribonuclease P (RNase P) is essential for protein synthesis.
Rebecca Feyh +7 more
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A new role for proteins subunits of RNase P: stabilization of the telomerase holoenzyme [PDF]
Microbial Cell, 2020 RNase P, an RNA-protein complex, is essential for processing tRNAs. Three of the ten protein subunits of Saccharomyces cerevisiae RNase P (and a related complex, RNase MRP) co-purify with yeast telomerase, another RNA-protein complex.
P. Daniela Garcia, Virginia A. Zakian
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Engineering of RNase P Ribozymes for Therapy against Human Cytomegalovirus Infection [PDF]
VirusesNucleic acid-based gene interference and editing strategies, such as antisense oligonucleotides, ribozymes, RNA interference (RNAi), and CRISPR/Cas9 coupled with guide RNAs, are exciting research tools and show great promise for clinical applications in ...
Adam Smith +3 more
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A RNase P Ribozyme Inhibits Gene Expression and Replication of Hepatitis B Virus in Cultured Cells [PDF]
Microorganisms, 2023 Hepatitis B virus (HBV), an international public health concern, is a leading viral cause of liver disease, such as hepatocellular carcinoma. Sequence-specific ribozymes derived from ribonuclease P (RNase P) catalytic RNA are being explored for gene ...
Bin Yan +3 more
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Towards plant resistance to viruses using protein-only RNase P [PDF]
Nature Communications, 2021 New approaches to plant disease control are important for pathogens that are difficult to control by existing methods. Here, the authors report a potential strategy to combat plant viruses by cytosolic expressed protein-only RNase P and show its ability ...
Anthony Gobert +8 more
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P finder: genomic and metagenomic annotation of RNase P RNA gene (rnpB) [PDF]
BMC Genomics, 2020 Background The rnpB gene encodes for an essential catalytic RNA (RNase P). Like other essential RNAs, RNase P’s sequence is highly variable. However, unlike other essential RNAs (i.e.
J. Christopher Ellis
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The DnaJK chaperone of Bacillus subtilis post-transcriptionally regulates gene expression through the YlxR(RnpM)/RNase P complex [PDF]
mBioTo survive in harsh natural environments, translation and mRNA metabolism must be tightly and coordinately controlled, as saving biological costs increases fitness. However, the roles of protein chaperones in this control system are unclear.
Mitsuo Ogura +3 more
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Structural basis of transfer RNA processing by bacterial minimal RNase P [PDF]
Nature CommunicationsPrecursor tRNAs (pre-tRNAs) require nucleolytic removal of 5′-leader and 3′-trailer sequences for maturation, which is essential for proper tRNA function. The endoribonuclease RNase P exists in diverse forms, including RNA- and protein-based RNase P, and
Takamasa Teramoto +7 more
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