Results 111 to 120 of about 455,663 (298)

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Identification of RMP24 and RMP64, human ribonuclease MRP-specific protein components

Cell Reports
Summary: Human RNase MRP is a ribonucleoprotein (RNP) enzyme that processes precursor rRNA (pre-rRNA) at ITS1 site 2 and may have additional activities. It is an endonuclease related to RNase P, which processes pre-tRNAs and pre-tRNA-like substrates.
Rui Che, Bhoomi Mirani, Monireh Panah, Xiaotong Chen, Hong Luo, Andrei Alexandrov   +5 more
doaj   +1 more source

Characterization of one new non-S-RNase of Armeniaca cathayana

Czech Journal of Genetics and Plant Breeding, 2019
Armeniaca cathayana, a new species described in 2010, belongs to gametophytic self-incompatibility (GSI) system which is under S-allele control. One new non-S-ribonuclease (non-S-RNase) was found in A. cathayana through comparing its nucleotide and amino
Mengpei Liu, Pei Hou, Xiaoyuan Wang, Yu Dong, Wei Zong   +4 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Endothelial Cells Angiogenesis in Sulfated Glycosaminoglycan (GAG) Hydrogels Enhanced by Bioactive Glass‐Released Ions

Advanced Functional Materials, EarlyView.
A mechanically tunable hydrogel composed of gelatin, chondroitin sulfate and laminin promotes angiogenesis in vitro without the supplement of growth factors. Endothelial cells morphogenesis was further enhanced by medium conditioned with bioactive glass 58S‐released ions (Ca and Si), thus offering a promising strategy to vascularize 3D tissue ...
Marco Piazzoni, Ilaria Borghi, Francesca Cadamuro, Sophia Dalfino, Riccardo Campanile, Sofia Nizzolo, Valeria Cassina, Francesca Tallia, Julian R. Jones, Francesco Mantegazza, Sabrina Bertini, Lorenzo Moroni, Francesco Nicotra, Laura Russo   +13 more
wiley   +1 more source

The solution structure of the prototype foamy virus RNase H domain indicates an important role of the basic loop in substrate binding

Retrovirology, 2012
Background The ribonuclease H (RNase H) domains of retroviral reverse transcriptases play an essential role in the replication cycle of retroviruses. During reverse transcription of the viral genomic RNA, an RNA/DNA hybrid is created whose RNA strand ...
Leo Berit, Schweimer Kristian, Rösch Paul, Hartl Maximilian J, Wöhrl Birgitta M   +4 more
doaj   +1 more source

Multiplexed in-gel microfluidic immunoassays: characterizing protein target loss during reprobing of benzophenone-modified hydrogels. [PDF]

, 2019
From whole tissues to single-cell lysate, heterogeneous immunoassays are widely utilized for analysis of protein targets in complex biospecimens. Recently, benzophenone-functionalized hydrogel scaffolds have been used to immobilize target protein for ...
Gopal, Anjali, Herr, Amy E
core   +1 more source

HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis [PDF]

, 2018
Cytokinesis, the final phase of cell division, is necessary to form two distinct daughter cells with correct distribution of genomic and cytoplasmic materials.
A Konishi, A Verdina, AC Borisa, AT Lau, B Mierzwa, C Ditchfield, C Lazzari, C Mollinari, C Norden, C Rinaldo, C Rinaldo, C Rinaldo, C Rinaldo, Cinzia Rinaldo, CK Hu, D Valente, Davide Valente, E Gresko, F Siepi, G D’Orazi, G D’Orazi, G Normand, GE Atilla-Gokcumen, H Goto, HC Huang, I Vitale, JA Blaquiere, JM Cummins, JP Fededa, JR Bischoff, K Kobiyama, L Vega De la, Laura Monteonofrio, M Agromayor, M Carmena, M Glotzer, M Kollareddy, Manuela Ferrara, Marco Crescenzi, ME Douglas, ME Douglas, O Afonso, P Carpinelli, PP D’Avino, R Kuriyama, R Nunes Bastos, Roberta Miscione, S Francia, S Hauf, S Matt, Serena Camerini, Silvia Soddu, SM Lens, TD Pollard, TG Hofmann, TG Hofmann, V Bavetsias, VV Saul, VV Saul, WM Zhao, XL Li, Y Feng, Y Kuwano, Y Terada, YH Kim, Z Storchova   +65 more
core   +1 more source

RNA‐Micelles as Self‐Assembling Structures for Efficient Co‐Delivery of Synergistic siRNA and Nucleoside Analogues to Treat CRC Lung Metastasis

Advanced Functional Materials, EarlyView.
Two kinds of self‐assembled RNA micelles were used to co‐deliver synergistic siRNA and nucleoside analogues for the treatment of colorectal cancer lung metastases. Near‐complete elimination of lung cancer metastases was confirmed in an orthotopic lung metastasis model constructed using human colorectal cancer lung metastases patient surgical samples to
Kai Jin, Piotr Rychahou, Daniel W. Binzel, B. Mark Evers, Peixuan Guo   +4 more
wiley   +1 more source