Decay-Initiating Endoribonucleolytic Cleavage by RNase Y Is Kept under Tight Control via Sequence Preference and Sub-cellular Localisation. [PDF]
PLoS Genetics, 2015 Bacteria depend on efficient RNA turnover, both during homeostasis and when rapidly altering gene expression in response to changes. Nevertheless, remarkably few details are known about the rate-limiting steps in targeting and decay of RNA.
Vanessa Khemici +3 more
doaj +6 more sources
Three essential ribonucleases-RNase Y, J1, and III-control the abundance of a majority of Bacillus subtilis mRNAs. [PDF]
PLoS Genetics, 2012 Bacillus subtilis possesses three essential enzymes thought to be involved in mRNA decay to varying degrees, namely RNase Y, RNase J1, and RNase III. Using recently developed high-resolution tiling arrays, we examined the effect of depletion of each of ...
Sylvain Durand +4 more
doaj +7 more sources
Correction: Decay-Initiating Endoribonucleolytic Cleavage by RNase Y Is Kept under Tight Control via Sequence Preference and Sub-cellular Localisation. [PDF]
PLoS Genetics, 2016 [This corrects the article DOI: 10.1371/journal.pgen.1005577.].
Vanessa Khemici +3 more
doaj +4 more sources
Escherichia coli RNase E can efficiently replace RNase Y in Bacillus subtilis. [PDF]
Nucleic Acids Res, 2021 AbstractRNase Y and RNase E are disparate endoribonucleases that govern global mRNA turnover/processing in the two evolutionary distant bacteria Bacillus subtilis and Escherichia coli, respectively. The two enzymes share a similar in vitro cleavage specificity and subcellular localization.
Laalami S +4 more
europepmc +4 more sources
Interaction of Bacillus subtilis Polynucleotide Phosphorylase and RNase Y: STRUCTURAL MAPPING AND EFFECT ON mRNA TURNOVER. [PDF]
J Biol Chem, 2016 Polynucleotide phosphorylase (PNPase), a 3'-to-5' phosphorolytic exoribonuclease, is thought to be the primary enzyme responsible for turnover ofBacillus subtilismRNA. The role of PNPase inB. subtilismRNA decay has been analyzed previously by comparison of mRNA profiles in a wild-type strainversusa strain that is deleted forpnpA, the gene encoding ...
Salvo E +4 more
europepmc +4 more sources
RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B. [PDF]
RNA Biol, 2018 Endoribonuclease Y (RNase Y) is a crucial regulator of virulence in Gram-positive bacteria. In the human pathogen Streptococcus pyogenes, RNase Y is required for the expression of the major secreted virulence factor streptococcal pyrogenic exotoxin B (SpeB), but the mechanism involved in this regulation remains elusive. Here, we demonstrate that the 5′
Broglia L +5 more
europepmc +8 more sources
Role of RNase Y in Clostridium perfringens mRNA Decay and Processing. [PDF]
J Bacteriol, 2017 ABSTRACT RNase Y is a major endoribonuclease that plays a crucial role in mRNA degradation and processing. We study the role of RNase Y in the Gram-positive anaerobic pathogen Clostridium perfringens , which until now has not been well understood.
Obana N, Nakamura K, Nomura N.
europepmc +4 more sources
Quasi-essentiality of RNase Y in Bacillus subtilis is caused by its critical role in the control of mRNA homeostasis. [PDF]
Nucleic Acids Res, 2021 Abstract RNA turnover is essential in all domains of life. The endonuclease RNase Y (rny) is one of the key components involved in RNA metabolism of the model organism Bacillus subtilis. Essentiality of RNase Y has been a matter of discussion, since deletion of the rny gene is possible, but leads to severe phenotypic effects.
Benda M +10 more
europepmc +5 more sources
Multiple roles of RNase Y in Streptococcus pyogenes mRNA processing and degradation. [PDF]
J Bacteriol, 2013 ABSTRACT Control over mRNA stability is an essential part of gene regulation that involves both endo- and exoribonucleases. RNase Y is a recently identified endoribonuclease in Gram-positive bacteria, and an RNase Y ortholog has been identified in Streptococcus pyogenes (group A streptococcus [GAS]).
Chen Z +4 more
europepmc +4 more sources
Downstream element determines RNase Y cleavage of the saePQRS operon in Staphylococcus aureus. [PDF]
Nucleic Acids Res, 2017 In gram-positive bacteria, RNase J1, RNase J2 and RNase Y are thought to be major contributors to mRNA degradation and maturation. In Staphylococcus aureus, RNase Y activity is restricted to regulating the mRNA decay of only certain transcripts. Here the saePQRS operon was used as a model to analyze RNase Y specificity in living cells.
Marincola G, Wolz C.
europepmc +5 more sources