Results 21 to 30 of about 321,082 (269)
Membrane Localization of RNase Y Is Important for Global Gene Expression in Bacillus subtilis. [PDF]
Int J Mol SciAbstract: RNase Y is an endoribonuclease that governs global mRNA turnover/processing in Ba-cillus subtilis and likely many other bacteria. The enzyme is anchored at the cell membrane, a pseudo-compartmentalization that appears coherent with its task to initiate decay of mRNAs translated predominantly at the cell periphery.
Laalami S +3 more
europepmc +4 more sources
Dissociation of the Dimer of the Intrinsically Disordered Domain of RNase Y upon Antibody Binding. [PDF]
Biophys J, 2018 Although RNase Y acts as the key enzyme initiating messenger RNA decay in Bacillus subtilis and likely in many other Gram-positive bacteria, its three-dimensional structure remains unknown. An antibody belonging to the rare immunoglobulin G (IgG) 2b λx isotype was raised against a 12-residue conserved peptide from the N-terminal noncatalytic domain of ...
Hardouin P +7 more
europepmc +4 more sources
Enzymatic activity necessary to restore the lethality due to Escherichia coli RNase E deficiency is distributed among bacteria lacking RNase E homologues. [PDF]
PLoS ONE, 2017 Escherichia coli RNase E (Eco-RNase E), encoded by rne (Eco-rne), is considered the global RNA decay initiator. Although Eco-RNase E is an essential gene product in E.
Masaru Tamura +4 more
doaj +1 more source
Initiation of decay of Bacillus subtilis rpsO mRNA by endoribonuclease RNase Y. [PDF]
J Bacteriol, 2010 ABSTRACT rpsO mRNA, a small monocistronic mRNA that encodes ribosomal protein S15, was used to study aspects of mRNA decay initiation in Bacillus subtilis . Decay of rpsO mRNA in a panel of 3′-to-5′ exoribonuclease mutants was analyzed using a 5′-proximal oligonucleotide
Yao S, Bechhofer DH.
europepmc +4 more sources
SR7 – a dual-function antisense RNA from Bacillus subtilis
RNA Biology, 2021 Here, we describe SR7, a dual-function antisense RNA encoded on the Bacillus subtilis chromosome. This RNA was earlier described as SigB-dependent regulatory RNA S1136 and reported to reduce the amount of the small ribosomal subunit under ethanol stress.
Inam Ul Haq +2 more
doaj +1 more source
RNase Y, a novel endoribonuclease, initiates riboswitch turnover in Bacillus subtilis [PDF]
The EMBO Journal, 2009 In contrast to Escherichia coli, initiation of mRNA decay in Gram-positive organisms is poorly understood. We studied the fate of the highly structured RNAs generated by premature transcription termination of S-adenosylmethionine (SAM)-dependent riboswitches in Bacillus subtilis. An essential protein of earlier unknown function, YmdA, was identified as
Karen, Shahbabian +3 more
openaire +2 more sources
RNase Y in Bacillus subtilis: a Natively Disordered Protein That Is the Functional Equivalent of RNase E from Escherichia coli [PDF]
Journal of Bacteriology, 2011 ABSTRACTThe control of mRNA stability is an important component of regulation in bacteria. Processing and degradation of mRNAs are initiated by an endonucleolytic attack, and the cleavage products are processively degraded by exoribonucleases. In many bacteria, these RNases, as well as RNA helicases and other proteins, are organized in a protein ...
Lehnik-Habrink, Martin +8 more
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Decay of a Model mRNA in Bacillus subtilis by a Combination of RNase J1 5′ Exonuclease and RNase Y Endonuclease Activities [PDF]
Journal of Bacteriology, 2011 ABSTRACT The involvement of the recently characterized 5′ exonuclease activity of RNase J1 and endonuclease activity of RNase Y in the turnover of Δ ermC mRNA in Bacillus subtilis was investigated. Evidence is presented that both of these activities determine the half-life of Δ
Shiyi, Yao +3 more
openaire +2 more sources
Microorganisms, 2021 Moonlighting proteins are proteins with more than one function. During the past 25 years, they have been found to be rather widespread in bacteria. In Bacillus subtilis, moonlighting has been disclosed to occur via DNA, protein or RNA binding or protein ...
Inam Ul Haq, Sabine Brantl
doaj +1 more source
Cells, 2022 Bacteria are dependent on rapid alterations in gene expression. A prerequisite for rapid adaptations is efficient RNA turnover, with endonuclease RNase Y playing a crucial role in mRNA stability as well as in maturation.
Rebecca Hinrichs +3 more
doaj +1 more source