Results 1 to 10 of about 76,507 (167)

BISR-RNAseq: an efficient and scalable RNAseq analysis workflow with interactive report generation [PDF]

BMC Bioinformatics, 2019
Background RNA sequencing has become an increasingly affordable way to profile gene expression patterns. Here we introduce a workflow implementing several open-source softwares that can be run on a high performance computing environment.
Venkat Sundar Gadepalli, Hatice Gulcin Ozer, Ayse Selen Yilmaz, Maciej Pietrzak, Amy Webb   +4 more
doaj   +4 more sources

T-RHEX-RNAseq – a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells

BMC Genomics, 2023
Background RNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming – to allow analysis of a broader range of transcripts – remains challenging.
Charlotte Gustafsson, Julia Hauenstein, Nicolai Frengen, Aleksandra Krstic, Sidinh Luc, Robert Månsson   +5 more
doaj   +4 more sources

High RNA quality extracted from the tolerant crop Cyamopsis tetragonoloba (L.) despite possession of low RNA integrity number

Biotechnology & Biotechnological Equipment, 2021
Salinity is one of the most limiting constraints of crop production. Nevertheless, highly-tolerant crop plants (e.g. ‘Guar’ Cyamopsis tetragonoloba) can thrive well under salinity conditions.
Abdel-Rhman Zakaria Gaafar, Fahad Al-Qurainy, Aref Alshameri, Salim Khan, Mohammad Nadeem, Mohamed Tarroum, Saleh Alansi, Hassan O. Shaikhaldein, Abdalrhaman M. Salih, Norah Arrak Alenezi   +9 more
doaj   +1 more source

Microrna expression patterns associated with average daily weight gain in kangal akkaraman lambs [PDF]

Eurasian Journal of Veterinary Sciences, 2022
Aim: This study aimed to identify miRNAs expressed in Kangal Akkaraman lambs and to identify miRNAs that may be associated with average daily weight gain (ADWG).
Gonca Sonmez, Seref Inal
doaj  

Integrative transcriptomic profiling of a mouse model of hypertension-accelerated diabetic kidney disease

Disease Models & Mechanisms, 2021
The current understanding of molecular mechanisms driving diabetic kidney disease (DKD) is limited, partly due to the complex structure of the kidney.
Frederikke E. Sembach, Helene M. Ægidius, Lisbeth N. Fink, Thomas Secher, Annemarie Aarup, Jacob Jelsing, Niels Vrang, Bo Feldt-Rasmussen, Kristoffer T. G. Rigbolt, Jens C. Nielsen, Mette V. Østergaard   +10 more
doaj   +1 more source

Transcriptome profile analysis reveals the emamectin benzoate-induced genes associated with olfaction and metabolic detoxification in Spodoptera exigua Hübner (Lepidoptera: noctuidae)

All Life, 2022
Emamectin benzoate (EMB) is one of the high-efficacious pesticide commonly used to control the ferocious economic crop pest S. exigua in the field. The results of S.
Yang Sun, Simin Tao, Wen Zhang, Bin Jiang, Han-Yang Dai, Bao-Sheng Liu, Yong-Jun Zhang, Xiang-dong Kong, Jing Zhao, Li-Xin Bai   +9 more
doaj   +1 more source

Photosynthetic response and transcriptomic profiling provide insights into the alkali tolerance of clone halophyte Leymus chinensis

Photosynthetica, 2020
Alkali stress is one of the important factors in restricting agriculture production. Leymus chinensis is constructive halophyte species in alkalized grassland in China. In order to investigate the gene expression response of L. chinensis to alkali stress,
H. WANG, Y. XIANG, L.H. LI, N. BHANBHRO, C.W. YANG, Z. ZHANG   +5 more
doaj   +1 more source

Rnaseq: Mrna To Illumina Library [PDF]

Protocol Exchange, 2015
This protocol details the RNAseq sample preparation starting with mRNA and ending with quality control of finished libraries. This protocol was used specifically for virus mRNA samples related to ORFeome lab group.
Marcia Sanders, Marcia Sanders, Anne Beall   +2 more
openaire   +2 more sources

A robust method to isolate Drosophila fat body nuclei for transcriptomic analysis

Fly, 2022
Gene expression profiles are typically described at the level of the tissue or, often in Drosophila, at the level of the whole organism. Collapsing the gene expression of entire tissues into single measures averages over potentially important ...
Vanika Gupta, Brian P. Lazzaro
doaj   +1 more source

Comparative Transcriptome Profiling Reveals Compatible and Incompatible Patterns of Potato Toward Phytophthora infestans

G3: Genes, Genomes, Genetics, 2020
Late blight, caused by Phytophthora infestans (P. infestans), is a devastating disease in potato worldwide. Our previous study revealed that the Solanum andigena genotype 03112-233 is resistant to P.
Yanfeng Duan, Shaoguang Duan, Miles R. Armstrong, Jianfei Xu, Jiayi Zheng, Jun Hu, Xinwei Chen, Ingo Hein, Guangcun Li, Liping Jin   +9 more
doaj   +1 more source