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RP-HPLC Binding Domains of Proteins
Analytical Chemistry, 1998Procedures have been developed to identify the chromatographic binding domains of horse heart cytochrome c (Cyt c) and bovine growth hormone (bGH) during their interaction with reversed-phase sorbent materials. The procedure involves adsorption of the protein solute to the chromatographic sorbent, followed by proteolytic cleavage.
Aguilar, Marie-Isabel +6 more
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Estimation of Lopinavir by RP-HPLC
Research Journal of Pharmacy and Technology, 2019Simple, sensitive and accurate HPLC method has been developed for the estimation of Lopinavir in pharmaceutical dosage forms. Lopinavir is an antiretroviral drug. Chromatographic separation was conducted on phenomenex lunar (250x4.6mm, 5µ) column at temperature 35°C, using a mobile phase composition of Acetonitrile: Water (70:30). The flow rate was 1ml/
D K Deepthi +3 more
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The RP-HPLC analysis of anthocyanins
Chromatographia, 1998Conditions were determined for the separation of a complex set of anthocyanins (free aglycones, mono- and multiglycosides and esterified forms) by HPLC. The optimised gradient elution method was then used to carry out qualitative and quantitative analysis of anthocyanin compounds present in the callus tissue ofRudbeckia hirta L. and the tubular flowers
M. Łuczkiewicz, W. Cisowski
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RP-HPLC of some metal phthalocyanines
Mikrochimica Acta, 1998The retention parameters of phthalocyanine, tetra-4-t-butylphthalocyanine and the latter's complexes with zinc, copper, rhodium and lutetium, as well as the copper complex of tetra-4-t-butyltetrabenzotriazaporphyrin were determined with μBondapak C18 sorbent.
Galina D. Brykina +2 more
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Packing Capillary Columns for RP-HPLC
Cold Spring Harbor Protocols, 2006INTRODUCTIONCapillary or microcolumn (<0.5-mm internal diameter [I.D.]) high-performance liquid chromatography (HPLC) is an extremely powerful technique for separating small quantities of proteins and peptides. This protocol describes a procedure for adapting conventional HPLC systems to provide accurate low-flow rates (0.4-4 μl/min) and gradients ...
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Multivariate quality assessment of aged RP-HPLC columns
Journal of Chemometrics, 1996The inner structure of RP-HPLC columns can change ('age') during routine use. The columns may be monitored to check the magnitude and type of the changes. One approach is to investigate, besides the chromatograms, the retention behaviour of a few selected solutes at different mobile phases (markers) during routine use. A distinction can be made between
Bolck, A. +3 more
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