Results 251 to 260 of about 97,740 (305)

RP-HPLC Binding Domains of Proteins

Analytical Chemistry, 1998
Procedures have been developed to identify the chromatographic binding domains of horse heart cytochrome c (Cyt c) and bovine growth hormone (bGH) during their interaction with reversed-phase sorbent materials. The procedure involves adsorption of the protein solute to the chromatographic sorbent, followed by proteolytic cleavage.
Aguilar, Marie-Isabel, Clayton, Daniel J., Holt, Phillip, Kronina, Veronica, Boysen, Reinhard I., Purcell, Anthony W., Hearn, Milton T. W.   +6 more
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Estimation of Lopinavir by RP-HPLC

Research Journal of Pharmacy and Technology, 2019
Simple, sensitive and accurate HPLC method has been developed for the estimation of Lopinavir in pharmaceutical dosage forms. Lopinavir is an antiretroviral drug. Chromatographic separation was conducted on phenomenex lunar (250x4.6mm, 5µ) column at temperature 35°C, using a mobile phase composition of Acetonitrile: Water (70:30). The flow rate was 1ml/
D K Deepthi, K Deepthi, M Jane, Hemanth Kumar   +3 more
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The RP-HPLC analysis of anthocyanins

Chromatographia, 1998
Conditions were determined for the separation of a complex set of anthocyanins (free aglycones, mono- and multiglycosides and esterified forms) by HPLC. The optimised gradient elution method was then used to carry out qualitative and quantitative analysis of anthocyanin compounds present in the callus tissue ofRudbeckia hirta L. and the tubular flowers
M. Łuczkiewicz, W. Cisowski
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RP-HPLC of some metal phthalocyanines

Mikrochimica Acta, 1998
The retention parameters of phthalocyanine, tetra-4-t-butylphthalocyanine and the latter's complexes with zinc, copper, rhodium and lutetium, as well as the copper complex of tetra-4-t-butyltetrabenzotriazaporphyrin were determined with μBondapak C18 sorbent.
Galina D. Brykina, Mariya I. Uvarova, Oleg A. Shpigun   +2 more
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Packing Capillary Columns for RP-HPLC

Cold Spring Harbor Protocols, 2006
INTRODUCTIONCapillary or microcolumn (<0.5-mm internal diameter [I.D.]) high-performance liquid chromatography (HPLC) is an extremely powerful technique for separating small quantities of proteins and peptides. This protocol describes a procedure for adapting conventional HPLC systems to provide accurate low-flow rates (0.4-4 μl/min) and gradients ...
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Multivariate quality assessment of aged RP-HPLC columns

Journal of Chemometrics, 1996
The inner structure of RP-HPLC columns can change ('age') during routine use. The columns may be monitored to check the magnitude and type of the changes. One approach is to investigate, besides the chromatograms, the retention behaviour of a few selected solutes at different mobile phases (markers) during routine use. A distinction can be made between
Bolck, A., Smilde, A. K., Duineveld, K. A. A., Coenegracht, P. M. J.   +3 more
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Assay of CCNU in Tumor by RP-HPLC

Artificial Cells, Blood Substitutes, and Biotechnology, 2005
This paper reports a RP-HPLC method for the determination of Lomustine (CCNU) in mouse tumor tissue. CCNU was separated and determined on a reverse-phase C18 5 microm column with a mobile phase of H2O-acetonitrile (54:46), detected at 254 nm and no internal standard. The calibration curve was linear (r = 0.9999) within the range of 0.98-15.68 microg/ml
Zhao, Z. Q., Sun, Y., Zhang, Q. Q., Jiang, J.   +3 more
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Purification of Large “Troublesome” Polypeptides by RP-HPLC

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol addresses the group of large polypeptides that have proved to be troublesome to handle because of their low solubility or the presence of secondary structural elements that favor supramolecular self-self assembly. Included in this group are polypeptides with amphipathic α-helical or β-sheet structures with extensive runs of ...
Hooi Hong, Keah, Milton T W, Hearn
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Some new selective stationary phases for RP‐HPLC

Journal of Separation Science, 2010
AbstractAt the present time, more complex analyses of apolar compounds with similar chemical structures or of polar compounds, especially basic ones, having diverse properties require more selective stationary phases having better stabilities. This paper describes several new stationary phases with directed selectivities that were prepared by ...
Isabel C S F, Jardim, Liane, Maldaner, Josimara, Lourenço, Livia M A, Fioravanti, Carol H, Collins   +4 more
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