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Analysis of RNA Polymerase Beta Subunit (rpoB) Gene Sequences for the Discriminative Power of Marine Vibrio Species

Microbial Ecology, 2009
In the present study, we sequenced the RNA polymerase beta subunit (rpoB) gene of marine Vibrio species and assessed its discriminative power in identifying vibrios.
Jang-Seu Ki, Pei-Yuan Qian
exaly   +2 more sources

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Molecular cloning and characterization of Borrelia burgdorferi rpoB

Gene, 1997
Borrelia burgdorferi rpoB, the gene encoding the beta-subunit of RNA polymerase, has been cloned and sequenced. The full-length gene encodes a protein of 1154 amino acids with a calculated molecular mass of 129.8 kDa. The amino-acid sequence is 49% identical to the corresponding protein from Escherichia coli. B.
M, Alekshun, M, Kashlev, I, Schwartz
openaire   +2 more sources

The unique organization of the rpoB region of Spiroplasma citri: a restriction and modification system gene is adjacent to rpoB

Gene, 1996
A 6.5-kb DNA fragment containing the gene (rpoB) encoding the RNA polymerase (RNAP) beta subunit, from the mollicute Spiroplasma citri (Sc), was cloned and sequenced. The classical eubacterial organization, with the genes (rplK, A, J and L) encoding ribosomal proteins L11, L1, L10 and L12 located immediately upstream from rpoB, was not found in the Sc ...
Laigret, Frédéric, Gaurivaud, P., Bové, J.M.   +2 more
openaire   +3 more sources

Association of rpoB mutations with rifampicin resistance in Mycobacterium avium

International Journal of Antimicrobial Agents, 2006
The susceptibility of clinical isolates of Mycobacterium avium to rifampicin (RIF) was examined. All 32 clinical isolates tested, including 18 from Japan, 13 from Poland and 1 from Thailand, were resistant to RIF (minimum inhibitory concentrations (MICs) > or =32 microg/mL for 17 isolates and 2-16 microg/mL for 15 isolates), whereas the type strain of ...
Saiko, Obata, Zofia, Zwolska, Emiko, Toyota, Koichiro, Kudo, Akio, Nakamura, Tetsuo, Sawai, Tadatoshi, Kuratsuji, Teruo, Kirikae   +7 more
openaire   +2 more sources

RifR mutations in the beginning of the Escherichia coli rpoB gene

Molecular and General Genetics MGG, 1994
In Escherichia coli, mutations conferring rifampicin (Rif) resistance map to the rpoB gene, which encodes the 1342-amino acid beta subunit of RNA polymerase. Almost all sequenced RifR mutations occur within the Rif region, encompassing rpoB codons 500-575.
K, Severinov, M, Soushko, A, Goldfarb, V, Nikiforov   +3 more
openaire   +2 more sources

Deletions, insertions and rearrangments affecting rpoB gene expression

Molecular and General Genetics MGG, 1979
Through cloning and deletion experiments on ColEl hybrids the rpoB gene (Rifr) was located on a physical restriction map; RNA polymerase binding studies showed no binding site within the structural gene. The genetic data and RNA polymerase binding studies lead to the conclusion that rp/L and rpoB are dependent upon a common promoter.
openaire   +2 more sources

Host associated polymorphisms in the Corynebacterium pseudotuberculosis rpoB gene sequence

Veterinary Microbiology, 2011
Corynebacterium pseudotuberculosis is a widespread facultative intracellular pathogen that causes caseous lymphadenitis disease in sheep and goats, and generates cutaneous abscesses and granulomas in horses and cattle. Although some genes have been studied for diagnostic and phylogenetic analysis within the genus Corynebacterium, at subspecies level ...
Retamal Merino, Patricio, Ríos Canales, Marco, Cheuquepán Valenzuela, Felipe, Abalos Pineda, Pedro, Pizarro Lucero, José, Borie Polanco, Consuelo, Gutiérrez Domínguez, Josefina   +6 more
openaire   +3 more sources

rpoB gene as a novel molecular marker to infer phylogeny in Planctomycetales

Antonie van Leeuwenhoek, 2013
The 16S rRNA gene has been used in the last decades as a gold standard for determining the phylogenetic position of bacteria and their taxonomy. It is a well conserved gene, with some variations, present in all bacteria and allows the reconstruction of genealogies of microorganisms.
Bondoso, Joana, Harder, Jens, Lage, Olga Maria   +2 more
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Molecular serotyping of Salmonella enterica by complete rpoB gene sequencing

Journal of Microbiology, 2012
Serotyping has been the gold standard for identifying Salmonella, but it requires large amounts of standard antisera. Multilocus sequence typing (MLST) has been applied to identify Salmonella serovars, but the recombination of 4-7 housekeeping genes and multiple analytic steps diminish its applicability. In the present study, we determined the complete
Won-Jin, Seong, Hyuk-Joon, Kwon, Tae-Eun, Kim, Deog-Yong, Lee, Mi-Sun, Park, Jae-Hong, Kim   +5 more
openaire   +2 more sources