Results 71 to 80 of about 508,064 (311)

Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNA. [PDF]

open access: yes, 2011
Accurate and rapid diagnosis of malaria infections is crucial for implementing species-appropriate treatment and saving lives. Molecular diagnostic tools are the most accurate and sensitive method of detecting Plasmodium, differentiating between ...
Jenna Oberstaller   +27 more
core   +1 more source

The Regulation of rRNA Gene Transcription during Directed Differentiation of Human Embryonic Stem Cells. [PDF]

open access: yesPLoS ONE, 2016
It has become increasingly clear that proper cellular control of pluripotency and differentiation is related to the regulation of rRNA synthesis. To further our understanding of the role that the regulation of rRNA synthesis has in pluripotency we ...
Jessica L Woolnough   +4 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Identification of a novel methyltransferase, Bmt2, responsible for the N-1-methyl-adenosine base modification of 25S rRNA in "Saccharomyces cerevisiae"

open access: yes, 2013
The 25S rRNA of yeast contains several base modifications in the functionally important regions. The enzymes responsible for most of these base modifications remained unknown.
Sharma, Sunny   +3 more
core   +1 more source

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]

open access: yes, 2012
As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205)   +7 more
core   +1 more source

Molecular cloning of the rRNA genes of Prevotella bryantii B14

open access: yesActa Agriculturae Slovenica, 2000
Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained.
Matjaž PETERKA, Gorazd AVGUŠTIN
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source

nf-core/rrna-ampliseq: rrna-ampliseq V1.0.0 "Olive Steel Panda"

open access: yes, 2018
<p>This is the initial stable release of rrna-ampliseq V 1.0.0 "Olive Steel Panda".</p> <p>This repository follows <a href="https://semver.org/">semantic versioning</a> guidelines to push new releases once available. We will
d4straub, Alexander Peltzer
core   +1 more source

Small RNA pathways in mammalian oocytes

open access: yesFEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Mapping of the Chemical Modifications of rRNAs

open access: yes, 2022
AbstractCellular RNAs, both coding and noncoding, contain several chemical modifications. Both ribose sugars and nitrogenous bases are targeted for these chemical additions. These modifications are believed to expand the topological potential of RNA molecules by bringing chemical diversity to otherwise limited repertoire.
Jun, Yang   +2 more
openaire   +2 more sources

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