Results 61 to 70 of about 223,842 (291)

Strategy for the identification of micro-organisms producing food and feed products : bacteria producing food enzymes as study case [PDF]

, 2020
Recent European regulations require safety assessments of food enzymes (FE) before their commercialization. FE are mainly produced by micro-organisms, whose viable strains nor associated DNA can be present in the final products.
De Keersmaecker, Sigrid C. J., Deckers, Marie, Deforce, Dieter, Denayer, Sarah, Fraiture, Marie-Alice, Heyndrickx, Marc, Roosens, Nancy H. C., Vanneste, Kevin, Winand, Raf   +8 more
core   +2 more sources

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Processing of preribosomal RNA in Saccharomyces cerevisiae [PDF]

, 2015
Most, if not all RNAs, are transcribed as precursors that require processing to gain functionality. Ribosomal RNAs (rRNA) from all organisms undergo both exo- and endonucleolytic processing.
Cruz, Jesús de la, Fernández-Pevida, Antonio, Kressler, Dieter   +2 more
core  

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Structural and mechanistic insights into ribosomal ITS2 RNA processing by nuclease-kinase machinery

eLife
Precursor ribosomal RNA (pre-rRNA) processing is a key step in ribosome biosynthesis and involves numerous RNases. A HEPN (higher eukaryote and prokaryote nucleotide binding) nuclease Las1 and a polynucleotide kinase Grc3 assemble into a tetramerase ...
Jiyun Chen, Hong Chen, Shanshan Li, Xiaofeng Lin, Rong Hu, Kaiming Zhang, Liang Liu   +6 more
doaj   +1 more source

From learning taxonomies to phylogenetic learning: integration of 16S rRNA gene data into FAME-based bacterial classification [PDF]

, 2010
Background: Machine learning techniques have shown to improve bacterial species classification based on fatty acid methyl ester (FAME) data. Nonetheless, FAME analysis has a limited resolution for discrimination of bacteria at the species level.
Bram Slabbinck, Willem Waegeman, Peter Dawyndt, Paul De Vos, Bernard De Baets, P Dawyndt, C Kunitsky, B Slabbinck, B Slabbinck, M Heyndrickx, P Kämpfer, DE Stead, M Vancanneyt, LG Wayne, E Stackebrandt, E Stackebrandt, KT Konstantinidis, KT Konstantinidis, E Pruesse, D Koller, A Mccallum, S Dumais, HP Kriegel, Z Barutcuoglu, N Cesa-Bianchi, J Rousu, C Vens, T Hofmann, F Wu, JS Lee, S Cheong, B Fei, R Craig, AC McHardy, N Diaz, SP Lapage, RR Sokal, N Saitou, J Felsenstein, J Felsenstein, P Dawyndt, R Kohavi, IH Witten, I Letunic, JP Euzéby, L Breiman, L Breiman, T Hastie, RO Duda, B Mirkin, B Slabbinck, BM Hansen, D Hutsebaut, JP Parker   +53 more
core   +3 more sources

Nucleolar Assembly of the Rrna Processing Machinery in Living Cells [PDF]

The Journal of Cell Biology, 2001
To understand how nuclear machineries are targeted to accurate locations during nuclear assembly, we investigated the pathway of the ribosomal RNA (rRNA) processing machinery towards ribosomal genes (nucleolar organizer regions [NORs]) at exit of mitosis.
T M, Savino, J, Gébrane-Younès, J, De Mey, J B, Sibarita, D, Hernandez-Verdun   +4 more
openaire   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Nuclear RNA Surveillance in \u3cem\u3eSaccharomyces cerevisiae\u3c/em\u3e: Trf4p-dependent Polyadenylation of Nascent Hypomethylated tRNA and an Aberrant Form of 5S rRNA [PDF]

, 2006
1-Methyladenosine modification at position 58 of tRNA is catalyzed by a two-subunit methyltransferase composed of Trm6p and Trm61p in Saccharomyces cerevisiae.
Anderson, James T., Kadaba, Sujatha A., Wang, Xuying   +2 more
core   +1 more source