Results 1 to 10 of about 37,713 (163)

Laboratory Evaluation of a SARS-CoV-2 RT-LAMP Test [PDF]

Tropical Medicine and Infectious Disease, 2023
There is a need to have more accessible molecular diagnostic tests for the diagnosis of severe acute respiratory syndrome coronavirus 2 disease in low- and middle-income countries.
Sandra Menting, Annette Erhart, Henk D. F. H. Schallig   +2 more
doaj   +2 more sources

Isothermal amplification and fluorescent detection of SARS-CoV-2 and SARS-CoV-2 variant virus in nasopharyngeal swabs. [PDF]

PLoS ONE, 2021
The COVID-19 pandemic caused by the SARS-CoV-2 is a serious health threat causing worldwide morbidity and mortality. Real-time reverse transcription PCR (RT-qPCR) is currently the standard for SARS-CoV-2 detection.
Les Jones, Abhijeet Bakre, Hemant Naikare, Ravindra Kolhe, Susan Sanchez, Yung-Yi C Mosley, Ralph A Tripp   +6 more
doaj   +2 more sources

A sensitive and simple RT-LAMP assay for sarbecovirus screening in bats. [PDF]

Microbiol Spectr, 2023
The availability of simple, inexpensive assays for coronavirus (CoV) detection is critical for conducting animal surveillance studies to prevent new zoonotic epidemics.
Chan TT-Y, Chow FW-N, Fung J, Cheng FK-K, Lo GC-S, Tsang C-C, Luk HK-H, Wong AC-P, He Z, Aw-Yong KL, Liu X, Yuen K-Y, Woo PC-Y, Lau SK-P.   +13 more
europepmc   +3 more sources

Developing RT-LAMP assays for rapid diagnosis of SARS-CoV-2 in saliva

EBioMedicine, 2022
Summary: Background: The coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has killed millions of people worldwide. The current crisis has created an unprecedented demand for rapid test of SARS-CoV-2 infection. Methods: Reverse transcription loop-
Nahed Ismail, Xiaowei Wang
exaly   +3 more sources

Development of an RT-LAMP Assay for the Rapid Detection of SFTS Virus

Viruses, 2021
Detection of severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) during the early phase of the disease is important for appropriate treatment, infection control, and prevention of further transmission. The reverse transcription loop-mediated
Shiori Sano, Shuetsu Fukushi, Souichi Yamada, Shizuko Harada, Hitomi Kinoshita, Satoko Sugimoto, Tomoki Yoshikawa, Takeshi Kurosu, Yuki Takamatsu, Masayuki Shimojima, Shoichi Toda, Yuka Hamada, Naoki Fujisawa, Takayuki Sugimoto, Masayuki Saijo   +14 more
doaj   +1 more source

Rapid and convenient detection of SARS-CoV-2 using a colorimetric triple-target reverse transcription loop-mediated isothermal amplification method [PDF]

PeerJ, 2022
Coronavirus Disease 2019 (COVID-19) caused by SARS-CoV-2 poses a significant threat to global public health. Early detection with reliable, fast, and simple assays is crucial to contain the spread of SARS-CoV-2.
Zhu Yang, Nicole Y. Liu, Zhiwei Zhu, Minmin Xiao, Shuzhi Zhong, Qiqi Xue, Lina Nie, Jinhong Zhao   +7 more
doaj   +2 more sources

Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection

International Journal of Infectious Diseases, 2021
Objective: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated to diagnose several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 in real-life clinical settings remains unclear. This study
Masato Inaba, Yuki Higashimoto, Yoko Toyama, Tomoya Horiguchi, Masaya Hibino, Mitsunaga Iwata, Kazuyoshi Imaizumi, Yohei Doi   +7 more
doaj   +1 more source

RT-LAMP Multicenter Study for SARS-CoV-2 Genome Molecular Detection in Brazilian Swab and Saliva Samples

Diagnostics, 2023
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a rapid method that can replace RT-qPCR. A simple molecular assay for SARS-CoV-2 RNA detection in gold-standard diagnosis through swabs and alternative specimens such as saliva ...
Vanessa Duarte da Costa, Alanna Calheiros Santos, Lucas Lima da Silva, Wilian Jean Wiggers, Claudia Alexandra Pontes Ivantes, Danielle Malta Lima, Jeová Keny Baima Colares, Deusilene Souza Vieira Dallacqua, Ana Rita Coimbra Motta-Castro, Alberto Martín Rivera Dávila, Sheila Araujo Teles, Megmar Aparecida dos Santos Carneiro, Karlla Antonieta Amorim Caetano, Fernando Antonio Costa Anunciação, Vanessa Salete de Paula, Livia Melo Villar, on behalf of The Brazilian COVID-19 Research Group   +16 more
doaj   +1 more source

Development and Clinical Application of a Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for SARS-CoV-2 Infection

mSphere, 2020
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak urgently necessitates sensitive and convenient COVID-19 diagnostics for the containment and timely treatment of patients.
Xuejiao Hu, Qianyun Deng, Junmin Li, Jierong Chen, Zixia Wang, Xiqin Zhang, Zhixin Fang, Haijian Li, Yunhu Zhao, Pan Yu, Wenmin Li, Xiaoming Wang, Shan Li, Lei Zhang, Tieying Hou   +14 more
doaj   +3 more sources

Evaluation of a saliva molecular point of care for the detection of SARS-CoV-2 in ambulatory care

Scientific Reports, 2021
Rapid identification of SARS-CoV-2-infected individuals is a cornerstone for the control of virus spread. The sensitivity of SARS-CoV-2 RNA detection by RT-PCR is similar in saliva and nasopharyngeal swabs.
Jérôme LeGoff, Solen Kernéis, Caroline Elie, Séverine Mercier-Delarue, Nabil Gastli, Laure Choupeaux, Jacques Fourgeaud, Marie-Laure Alby, Pierre Quentin, Juliette Pavie, Patricia Brazille, Marie Laure Néré, Marine Minier, Audrey Gabassi, Chrystel Leroy, Béatrice Parfait, Jean-Marc Tréluyer, Constance Delaugerre   +17 more
doaj   +1 more source