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Laboratory Evaluation of a SARS-CoV-2 RT-LAMP Test [PDF]

open access: yesTropical Medicine and Infectious Disease, 2023
There is a need to have more accessible molecular diagnostic tests for the diagnosis of severe acute respiratory syndrome coronavirus 2 disease in low- and middle-income countries.
Sandra Menting   +2 more
doaj   +5 more sources

A sensitive and simple RT-LAMP assay for sarbecovirus screening in bats. [PDF]

open access: yesMicrobiol Spectr, 2023
The availability of simple, inexpensive assays for coronavirus (CoV) detection is critical for conducting animal surveillance studies to prevent new zoonotic epidemics.
Chan TT-Y   +13 more
europepmc   +7 more sources

Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system for a highly sensitive detection of enterovirus in the stool samples of acute flaccid paralysis cases [PDF]

open access: yesBMC Infectious Diseases, 2009
Background In the global eradication program for poliomyelitis, the laboratory diagnosis plays a critical role by isolating poliovirus (PV) from the stool samples of acute flaccid paralysis (AFP) cases. In this study, we developed a reverse transcription-
Wakita Takaji   +6 more
doaj   +4 more sources

Isothermal amplification and fluorescent detection of SARS-CoV-2 and SARS-CoV-2 variant virus in nasopharyngeal swabs. [PDF]

open access: yesPLoS ONE, 2021
The COVID-19 pandemic caused by the SARS-CoV-2 is a serious health threat causing worldwide morbidity and mortality. Real-time reverse transcription PCR (RT-qPCR) is currently the standard for SARS-CoV-2 detection.
Les Jones   +6 more
doaj   +2 more sources

Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification. [PDF]

open access: yesPLoS Neglected Tropical Diseases, 2020
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly pathogenic novel coronavirus that has caused a worldwide outbreak. Here we describe a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that uses a ...
Rokusuke Yoshikawa   +5 more
doaj   +3 more sources

Development of an RT-LAMP Assay for the Rapid Detection of SFTS Virus

open access: yesViruses, 2021
Detection of severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) during the early phase of the disease is important for appropriate treatment, infection control, and prevention of further transmission. The reverse transcription loop-mediated
Shiori Sano   +14 more
doaj   +1 more source

Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection

open access: yesInternational Journal of Infectious Diseases, 2021
Objective: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated to diagnose several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 in real-life clinical settings remains unclear. This study
Masato Inaba   +7 more
doaj   +1 more source

Rapid and convenient detection of SARS-CoV-2 using a colorimetric triple-target reverse transcription loop-mediated isothermal amplification method [PDF]

open access: yesPeerJ, 2022
Coronavirus Disease 2019 (COVID-19) caused by SARS-CoV-2 poses a significant threat to global public health. Early detection with reliable, fast, and simple assays is crucial to contain the spread of SARS-CoV-2.
Zhu Yang   +7 more
doaj   +2 more sources

RT-LAMP Multicenter Study for SARS-CoV-2 Genome Molecular Detection in Brazilian Swab and Saliva Samples

open access: yesDiagnostics, 2023
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a rapid method that can replace RT-qPCR. A simple molecular assay for SARS-CoV-2 RNA detection in gold-standard diagnosis through swabs and alternative specimens such as saliva ...
Vanessa Duarte da Costa   +16 more
doaj   +1 more source

Development and Clinical Application of a Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for SARS-CoV-2 Infection

open access: yesmSphere, 2020
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak urgently necessitates sensitive and convenient COVID-19 diagnostics for the containment and timely treatment of patients.
Xuejiao Hu   +14 more
doaj   +3 more sources

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